{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 1,
   "id": "9da32929-b7eb-46e0-afca-81b118b27546",
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/home/user4/miniconda3/envs/protein/lib/python3.10/site-packages/tqdm/auto.py:21: TqdmWarning: IProgress not found. Please update jupyter and ipywidgets. See https://ipywidgets.readthedocs.io/en/stable/user_install.html\n",
      "  from .autonotebook import tqdm as notebook_tqdm\n"
     ]
    }
   ],
   "source": [
    "import pandas as pd\n",
    "import os\n",
    "import shutil\n",
    "import json\n",
    "import esm\n",
    "import numpy as np"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "id": "51449a26-42b4-4b55-b474-7c665eaefb81",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "('a.ef', '.pdb')"
      ]
     },
     "execution_count": 2,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "os.path.splitext(\"a.ef.pdb\")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "id": "d164969a-2191-499b-b5c4-bc944333ccd1",
   "metadata": {},
   "outputs": [],
   "source": [
    "proteins = os.listdir(\"data/\")\n",
    "for p in proteins:\n",
    "    try:\n",
    "        os.rename(f\"data/{p}/{p}_detail.fasta\", f\"data/{p}/{p}.detail.json\")\n",
    "        os.rename(f\"data/{p}/{p}-info.json\", f\"data/{p}/{p}.info.json\")\n",
    "    except:\n",
    "        print(p)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "id": "18663d7b-98a8-4555-8eb2-0c6700a9d515",
   "metadata": {},
   "outputs": [],
   "source": [
    "import biotite.structure\n",
    "from biotite.structure.io import pdbx, pdb\n",
    "from biotite.structure.residues import get_residues\n",
    "from biotite.structure import filter_backbone\n",
    "from biotite.structure import get_chains\n",
    "from biotite.sequence import ProteinSequence\n",
    "import numpy as np\n",
    "from scipy.spatial import transform\n",
    "from scipy.stats import special_ortho_group\n",
    "import torch\n",
    "import torch.nn as nn\n",
    "import torch.nn.functional as F\n",
    "import torch.utils.data as data\n",
    "from typing import Sequence, Tuple, List\n",
    "\n",
    "from esm.data import BatchConverter\n",
    "\n",
    "\n",
    "def load_structure(fpath, chain=None):\n",
    "    \"\"\"\n",
    "    Args:\n",
    "        fpath: filepath to either pdb or cif file\n",
    "        chain: the chain id or list of chain ids to load\n",
    "    Returns:\n",
    "        biotite.structure.AtomArray\n",
    "    \"\"\"\n",
    "    if fpath.endswith('cif'):\n",
    "        with open(fpath) as fin:\n",
    "            pdbxf = pdbx.PDBxFile.read(fin)\n",
    "        structure = pdbx.get_structure(pdbxf, model=1)\n",
    "    elif fpath.endswith('pdb'):\n",
    "        with open(fpath) as fin:\n",
    "            pdbf = pdb.PDBFile.read(fin)\n",
    "        structure = pdb.get_structure(pdbf, model=1)\n",
    "    bbmask = filter_backbone(structure)\n",
    "    structure = structure[bbmask]\n",
    "    all_chains = get_chains(structure)\n",
    "    if len(all_chains) == 0:\n",
    "        raise ValueError('No chains found in the input file.')\n",
    "    if chain is None:\n",
    "        chain_ids = all_chains\n",
    "    elif isinstance(chain, list):\n",
    "        chain_ids = chain\n",
    "    else:\n",
    "        chain_ids = [chain] \n",
    "    for chain in chain_ids:\n",
    "        if chain not in all_chains:\n",
    "            raise ValueError(f'Chain {chain} not found in input file')\n",
    "    chain_filter = [a.chain_id in chain_ids for a in structure]\n",
    "    structure = structure[chain_filter]\n",
    "    return structure\n",
    "\n",
    "\n",
    "def get_atom_coords_residuewise(atoms: List[str], struct: biotite.structure.AtomArray):\n",
    "    \"\"\"\n",
    "    Example for atoms argument: [\"N\", \"CA\", \"C\"]\n",
    "    \"\"\"\n",
    "    def filterfn(s, axis=None):\n",
    "        filters = np.stack([s.atom_name == name for name in atoms], axis=1)\n",
    "        sum = filters.sum(0)\n",
    "        if not np.all(sum <= np.ones(filters.shape[1])):\n",
    "            raise RuntimeError(\"structure has multiple atoms with same name\")\n",
    "        index = filters.argmax(0)\n",
    "        coords = s[index].coord\n",
    "        coords[sum == 0] = float(\"nan\")\n",
    "        return coords\n",
    "\n",
    "    return biotite.structure.apply_residue_wise(struct, struct, filterfn)\n",
    "\n",
    "def extract_coords_from_structure(structure: biotite.structure.AtomArray):\n",
    "    \"\"\"\n",
    "    Args:\n",
    "        structure: An instance of biotite AtomArray\n",
    "    Returns:\n",
    "        Tuple (coords, seq)\n",
    "            - coords is an L x 3 x 3 array for N, CA, C coordinates\n",
    "            - seq is the extracted sequence\n",
    "    \"\"\"\n",
    "    coords = get_atom_coords_residuewise([\"N\", \"CA\", \"C\"], structure)\n",
    "    residue_identities = get_residues(structure)[1]\n",
    "    seq = ''.join([ProteinSequence.convert_letter_3to1(r) for r in residue_identities])\n",
    "    return coords, seq\n",
    "\n",
    "\n",
    "def load_coords(fpath, chain):\n",
    "    \"\"\"\n",
    "    Args:\n",
    "        fpath: filepath to either pdb or cif file\n",
    "        chain: the chain id\n",
    "    Returns:\n",
    "        Tuple (coords, seq)\n",
    "            - coords is an L x 3 x 3 array for N, CA, C coordinates\n",
    "            - seq is the extracted sequence\n",
    "    \"\"\"\n",
    "    structure = load_structure(fpath, chain)\n",
    "    return extract_coords_from_structure(structure)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "id": "e8af7bd0-13bb-4e3e-9aed-c63efe593d0a",
   "metadata": {},
   "outputs": [],
   "source": [
    "from Bio import PDB\n",
    "\n",
    "def get_seq_from_pdb(pdb_file):\n",
    "    # 创建一个PDB文件解析器\n",
    "    parser = PDB.PDBParser(QUIET=True)\n",
    "    \n",
    "    # 指定PDB文件的路径\n",
    "    pdb_file_path = pdb_file\n",
    "    \n",
    "    # 使用解析器读取PDB文件\n",
    "    structure = parser.get_structure(\"protein_structure\", pdb_file_path)\n",
    "    \n",
    "    # 提取蛋白质序列\n",
    "    protein_sequence = \"\"\n",
    "    for model in structure:\n",
    "        for chain in model:\n",
    "            for residue in chain:\n",
    "                if PDB.is_aa(residue):\n",
    "                    # 如果是氨基酸残基，则将其序列添加到蛋白质序列中\n",
    "                    protein_sequence += PDB.Polypeptide.three_to_one(residue.get_resname())\n",
    "    return protein_sequence\n",
    "    print(protein_sequence)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "id": "0827644c-bc8a-4972-b420-38fcae31563b",
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>NO</th>\n",
       "      <th>PROTEIN</th>\n",
       "      <th>UniProt_ID</th>\n",
       "      <th>MUTATION</th>\n",
       "      <th>SOURCE</th>\n",
       "      <th>PDB_wild</th>\n",
       "      <th>PDB_Chain_Mutation</th>\n",
       "      <th>SEC_STR</th>\n",
       "      <th>ASA</th>\n",
       "      <th>pH</th>\n",
       "      <th>T_(C)</th>\n",
       "      <th>∆Tm_(C)</th>\n",
       "      <th>∆∆G_(kcal/mol)</th>\n",
       "      <th>STATE</th>\n",
       "      <th>REVERSIBILITY</th>\n",
       "      <th>PubMed_ID</th>\n",
       "      <th>KEY_WORDS</th>\n",
       "      <th>REFERENCE</th>\n",
       "      <th>AUTHOR</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>44</td>\n",
       "      <td>Alpha/beta hydrolase family protein</td>\n",
       "      <td>W0TJ64</td>\n",
       "      <td>S176A (Based on Uniprot)</td>\n",
       "      <td>Saccharomonospora viridis</td>\n",
       "      <td>7CTS</td>\n",
       "      <td>-</td>\n",
       "      <td>Coil</td>\n",
       "      <td>6.00</td>\n",
       "      <td>8.0</td>\n",
       "      <td>-</td>\n",
       "      <td>80.4</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>No</td>\n",
       "      <td>-</td>\n",
       "      <td>crystal structure; disulfide bond; enzymatic a...</td>\n",
       "      <td>Proteins. 89(5):502-511 (2021) PMID: 33340163</td>\n",
       "      <td>Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>45</td>\n",
       "      <td>Alpha/beta hydrolase family protein</td>\n",
       "      <td>W0TJ64</td>\n",
       "      <td>S176A (Based on Uniprot)</td>\n",
       "      <td>Saccharomonospora viridis</td>\n",
       "      <td>7CTS</td>\n",
       "      <td>-</td>\n",
       "      <td>Coil</td>\n",
       "      <td>6.00</td>\n",
       "      <td>8.0</td>\n",
       "      <td>-</td>\n",
       "      <td>78.2</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>No</td>\n",
       "      <td>-</td>\n",
       "      <td>crystal structure; disulfide bond; enzymatic a...</td>\n",
       "      <td>Proteins. 89(5):502-511 (2021) PMID: 33340163</td>\n",
       "      <td>Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>46</td>\n",
       "      <td>Alpha/beta hydrolase family protein</td>\n",
       "      <td>W0TJ64</td>\n",
       "      <td>S176A (Based on Uniprot)</td>\n",
       "      <td>Saccharomonospora viridis</td>\n",
       "      <td>7CTS</td>\n",
       "      <td>-</td>\n",
       "      <td>Coil</td>\n",
       "      <td>6.00</td>\n",
       "      <td>8.0</td>\n",
       "      <td>-</td>\n",
       "      <td>78.4</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>No</td>\n",
       "      <td>-</td>\n",
       "      <td>crystal structure; disulfide bond; enzymatic a...</td>\n",
       "      <td>Proteins. 89(5):502-511 (2021) PMID: 33340163</td>\n",
       "      <td>Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>47</td>\n",
       "      <td>Alpha/beta hydrolase family protein</td>\n",
       "      <td>W0TJ64</td>\n",
       "      <td>S176A (Based on Uniprot)</td>\n",
       "      <td>Saccharomonospora viridis</td>\n",
       "      <td>7CTS</td>\n",
       "      <td>-</td>\n",
       "      <td>Coil</td>\n",
       "      <td>6.00</td>\n",
       "      <td>8.0</td>\n",
       "      <td>-</td>\n",
       "      <td>79.8</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>No</td>\n",
       "      <td>-</td>\n",
       "      <td>crystal structure; disulfide bond; enzymatic a...</td>\n",
       "      <td>Proteins. 89(5):502-511 (2021) PMID: 33340163</td>\n",
       "      <td>Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>48</td>\n",
       "      <td>Alpha/beta hydrolase family protein</td>\n",
       "      <td>W0TJ64</td>\n",
       "      <td>S176A (Based on Uniprot)</td>\n",
       "      <td>Saccharomonospora viridis</td>\n",
       "      <td>7CTS</td>\n",
       "      <td>-</td>\n",
       "      <td>Coil</td>\n",
       "      <td>6.00</td>\n",
       "      <td>8.0</td>\n",
       "      <td>-</td>\n",
       "      <td>80.4</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>No</td>\n",
       "      <td>-</td>\n",
       "      <td>crystal structure; disulfide bond; enzymatic a...</td>\n",
       "      <td>Proteins. 89(5):502-511 (2021) PMID: 33340163</td>\n",
       "      <td>Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7339</th>\n",
       "      <td>16383</td>\n",
       "      <td>Guanine nucleotide-binding protein G(i) subuni...</td>\n",
       "      <td>P63096</td>\n",
       "      <td>G352A(Based on UniProt and PDB)</td>\n",
       "      <td>Homo sapiens (Human)</td>\n",
       "      <td>3UMR</td>\n",
       "      <td>3umr_A:G352A</td>\n",
       "      <td>Helix</td>\n",
       "      <td>41.93</td>\n",
       "      <td>7.4</td>\n",
       "      <td>-</td>\n",
       "      <td>0</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>Unknown</td>\n",
       "      <td>26258638</td>\n",
       "      <td>G-protein; GPCR; alanine mutations; rhodopsin-...</td>\n",
       "      <td>Nat Struct Mol Biol 22(9):686-694 (2015) PMID:...</td>\n",
       "      <td>Sun, D., Flock, T., Deupi, X., Maeda, S., Matk...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7340</th>\n",
       "      <td>16384</td>\n",
       "      <td>Guanine nucleotide-binding protein G(i) subuni...</td>\n",
       "      <td>P63096</td>\n",
       "      <td>L353A(Based on UniProt and PDB)</td>\n",
       "      <td>Homo sapiens (Human)</td>\n",
       "      <td>3UMR</td>\n",
       "      <td>3umr_A:L353A</td>\n",
       "      <td>Coil</td>\n",
       "      <td>9.83</td>\n",
       "      <td>7.4</td>\n",
       "      <td>-</td>\n",
       "      <td>0.4</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>Unknown</td>\n",
       "      <td>26258638</td>\n",
       "      <td>G-protein; GPCR; alanine mutations; rhodopsin-...</td>\n",
       "      <td>Nat Struct Mol Biol 22(9):686-694 (2015) PMID:...</td>\n",
       "      <td>Sun, D., Flock, T., Deupi, X., Maeda, S., Matk...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7341</th>\n",
       "      <td>16385</td>\n",
       "      <td>Guanine nucleotide-binding protein G(i) subuni...</td>\n",
       "      <td>P63096</td>\n",
       "      <td>F354A(Based on UniProt and PDB)</td>\n",
       "      <td>Homo sapiens (Human)</td>\n",
       "      <td>3UMR</td>\n",
       "      <td>3umr_A:F354A</td>\n",
       "      <td>Coil</td>\n",
       "      <td>74.74</td>\n",
       "      <td>7.4</td>\n",
       "      <td>-</td>\n",
       "      <td>0</td>\n",
       "      <td>-</td>\n",
       "      <td>-</td>\n",
       "      <td>Unknown</td>\n",
       "      <td>26258638</td>\n",
       "      <td>G-protein; GPCR; alanine mutations; rhodopsin-...</td>\n",
       "      <td>Nat Struct Mol Biol 22(9):686-694 (2015) PMID:...</td>\n",
       "      <td>Sun, D., Flock, T., Deupi, X., Maeda, S., Matk...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14260</th>\n",
       "      <td>28441</td>\n",
       "      <td>Thermonuclease</td>\n",
       "      <td>P00644</td>\n",
       "      <td>F116C (Based on UniProt)</td>\n",
       "      <td>Staphylococcal</td>\n",
       "      <td>1stn</td>\n",
       "      <td>1stn_A:F34C</td>\n",
       "      <td>-</td>\n",
       "      <td>NaN</td>\n",
       "      <td>7.6</td>\n",
       "      <td>20</td>\n",
       "      <td>72</td>\n",
       "      <td>-2.8</td>\n",
       "      <td>-</td>\n",
       "      <td>Unknown</td>\n",
       "      <td>20194783</td>\n",
       "      <td>conformational stability, thermal stability, l...</td>\n",
       "      <td>PROC NATL ACAD SCI U S A 107, 4908-4913 (2010)...</td>\n",
       "      <td>Isom DG, Vardy E, Oas TG, Hellinga HW.</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14261</th>\n",
       "      <td>28442</td>\n",
       "      <td>Thermonuclease</td>\n",
       "      <td>P00644</td>\n",
       "      <td>L118C (Based on UniProt)</td>\n",
       "      <td>Staphylococcal</td>\n",
       "      <td>1stn</td>\n",
       "      <td>1stn_A:L36C</td>\n",
       "      <td>-</td>\n",
       "      <td>NaN</td>\n",
       "      <td>7.6</td>\n",
       "      <td>20</td>\n",
       "      <td>71</td>\n",
       "      <td>-2.9</td>\n",
       "      <td>-</td>\n",
       "      <td>Unknown</td>\n",
       "      <td>20194783</td>\n",
       "      <td>conformational stability, thermal stability, l...</td>\n",
       "      <td>PROC NATL ACAD SCI U S A 107, 4908-4913 (2010)...</td>\n",
       "      <td>Isom DG, Vardy E, Oas TG, Hellinga HW.</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>5285 rows × 19 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "          NO                                            PROTEIN UniProt_ID  \\\n",
       "7         44                Alpha/beta hydrolase family protein     W0TJ64   \n",
       "8         45                Alpha/beta hydrolase family protein     W0TJ64   \n",
       "9         46                Alpha/beta hydrolase family protein     W0TJ64   \n",
       "10        47                Alpha/beta hydrolase family protein     W0TJ64   \n",
       "11        48                Alpha/beta hydrolase family protein     W0TJ64   \n",
       "...      ...                                                ...        ...   \n",
       "7339   16383  Guanine nucleotide-binding protein G(i) subuni...     P63096   \n",
       "7340   16384  Guanine nucleotide-binding protein G(i) subuni...     P63096   \n",
       "7341   16385  Guanine nucleotide-binding protein G(i) subuni...     P63096   \n",
       "14260  28441                                     Thermonuclease     P00644   \n",
       "14261  28442                                     Thermonuclease     P00644   \n",
       "\n",
       "                              MUTATION                     SOURCE PDB_wild  \\\n",
       "7             S176A (Based on Uniprot)  Saccharomonospora viridis     7CTS   \n",
       "8             S176A (Based on Uniprot)  Saccharomonospora viridis     7CTS   \n",
       "9             S176A (Based on Uniprot)  Saccharomonospora viridis     7CTS   \n",
       "10            S176A (Based on Uniprot)  Saccharomonospora viridis     7CTS   \n",
       "11            S176A (Based on Uniprot)  Saccharomonospora viridis     7CTS   \n",
       "...                                ...                        ...      ...   \n",
       "7339   G352A(Based on UniProt and PDB)       Homo sapiens (Human)     3UMR   \n",
       "7340   L353A(Based on UniProt and PDB)       Homo sapiens (Human)     3UMR   \n",
       "7341   F354A(Based on UniProt and PDB)       Homo sapiens (Human)     3UMR   \n",
       "14260         F116C (Based on UniProt)             Staphylococcal     1stn   \n",
       "14261         L118C (Based on UniProt)             Staphylococcal     1stn   \n",
       "\n",
       "      PDB_Chain_Mutation SEC_STR    ASA   pH T_(C) ∆Tm_(C) ∆∆G_(kcal/mol)  \\\n",
       "7                      -    Coil   6.00  8.0     -    80.4              -   \n",
       "8                      -    Coil   6.00  8.0     -    78.2              -   \n",
       "9                      -    Coil   6.00  8.0     -    78.4              -   \n",
       "10                     -    Coil   6.00  8.0     -    79.8              -   \n",
       "11                     -    Coil   6.00  8.0     -    80.4              -   \n",
       "...                  ...     ...    ...  ...   ...     ...            ...   \n",
       "7339        3umr_A:G352A   Helix  41.93  7.4     -       0              -   \n",
       "7340        3umr_A:L353A    Coil   9.83  7.4     -     0.4              -   \n",
       "7341        3umr_A:F354A    Coil  74.74  7.4     -       0              -   \n",
       "14260        1stn_A:F34C       -    NaN  7.6    20      72           -2.8   \n",
       "14261        1stn_A:L36C       -    NaN  7.6    20      71           -2.9   \n",
       "\n",
       "      STATE REVERSIBILITY PubMed_ID  \\\n",
       "7         -            No         -   \n",
       "8         -            No         -   \n",
       "9         -            No         -   \n",
       "10        -            No         -   \n",
       "11        -            No         -   \n",
       "...     ...           ...       ...   \n",
       "7339      -       Unknown  26258638   \n",
       "7340      -       Unknown  26258638   \n",
       "7341      -       Unknown  26258638   \n",
       "14260     -       Unknown  20194783   \n",
       "14261     -       Unknown  20194783   \n",
       "\n",
       "                                               KEY_WORDS  \\\n",
       "7      crystal structure; disulfide bond; enzymatic a...   \n",
       "8      crystal structure; disulfide bond; enzymatic a...   \n",
       "9      crystal structure; disulfide bond; enzymatic a...   \n",
       "10     crystal structure; disulfide bond; enzymatic a...   \n",
       "11     crystal structure; disulfide bond; enzymatic a...   \n",
       "...                                                  ...   \n",
       "7339   G-protein; GPCR; alanine mutations; rhodopsin-...   \n",
       "7340   G-protein; GPCR; alanine mutations; rhodopsin-...   \n",
       "7341   G-protein; GPCR; alanine mutations; rhodopsin-...   \n",
       "14260  conformational stability, thermal stability, l...   \n",
       "14261  conformational stability, thermal stability, l...   \n",
       "\n",
       "                                               REFERENCE  \\\n",
       "7          Proteins. 89(5):502-511 (2021) PMID: 33340163   \n",
       "8          Proteins. 89(5):502-511 (2021) PMID: 33340163   \n",
       "9          Proteins. 89(5):502-511 (2021) PMID: 33340163   \n",
       "10         Proteins. 89(5):502-511 (2021) PMID: 33340163   \n",
       "11         Proteins. 89(5):502-511 (2021) PMID: 33340163   \n",
       "...                                                  ...   \n",
       "7339   Nat Struct Mol Biol 22(9):686-694 (2015) PMID:...   \n",
       "7340   Nat Struct Mol Biol 22(9):686-694 (2015) PMID:...   \n",
       "7341   Nat Struct Mol Biol 22(9):686-694 (2015) PMID:...   \n",
       "14260  PROC NATL ACAD SCI U S A 107, 4908-4913 (2010)...   \n",
       "14261  PROC NATL ACAD SCI U S A 107, 4908-4913 (2010)...   \n",
       "\n",
       "                                                  AUTHOR  \n",
       "7      Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...  \n",
       "8      Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...  \n",
       "9      Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...  \n",
       "10     Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...  \n",
       "11     Emori M, Numoto N, Senga A, Bekker GJ, Kamiya ...  \n",
       "...                                                  ...  \n",
       "7339   Sun, D., Flock, T., Deupi, X., Maeda, S., Matk...  \n",
       "7340   Sun, D., Flock, T., Deupi, X., Maeda, S., Matk...  \n",
       "7341   Sun, D., Flock, T., Deupi, X., Maeda, S., Matk...  \n",
       "14260             Isom DG, Vardy E, Oas TG, Hellinga HW.  \n",
       "14261             Isom DG, Vardy E, Oas TG, Hellinga HW.  \n",
       "\n",
       "[5285 rows x 19 columns]"
      ]
     },
     "execution_count": 4,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "df = pd.read_table(\"data_backend/1temp.tsv\")\n",
    "df = df[df['∆Tm_(C)'] != '-']\n",
    "df"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "id": "eab630b7-7a86-4d4e-9567-de13253eda61",
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "{'P0ABQ4-8.0': {'MUTATION': ['L24V',\n",
       "   'W30F',\n",
       "   'W30M',\n",
       "   'I41A',\n",
       "   'I41V',\n",
       "   'M42F',\n",
       "   'M42Y',\n",
       "   'H45S',\n",
       "   'W47L',\n",
       "   'W47V',\n",
       "   'L62A',\n",
       "   'L62V',\n",
       "   'C85L',\n",
       "   'I91A',\n",
       "   'I91G',\n",
       "   'R98Y',\n",
       "   'Q102L',\n",
       "   'Q102W',\n",
       "   'T113V',\n",
       "   'H114V',\n",
       "   'D116I',\n",
       "   'D116M',\n",
       "   'E154V',\n",
       "   'L156Y'],\n",
       "  '∆Tm': [54.8,\n",
       "   48.7,\n",
       "   48.4,\n",
       "   39.0,\n",
       "   53.3,\n",
       "   47.9,\n",
       "   44.2,\n",
       "   55.1,\n",
       "   50.6,\n",
       "   41.9,\n",
       "   42.0,\n",
       "   53.3,\n",
       "   42.4,\n",
       "   55.2,\n",
       "   49.8,\n",
       "   54.8,\n",
       "   57.0,\n",
       "   52.8,\n",
       "   52.5,\n",
       "   47.3,\n",
       "   50.3,\n",
       "   50.6,\n",
       "   53.9,\n",
       "   47.8],\n",
       "  'UniProt_ID': 'P0ABQ4',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 3.0,\n",
       "  'pH': 8.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': '-',\n",
       "  'PubMed_ID': nan,\n",
       "  'KEY_WORDS': 'environmental constraints; mutational landscape; functional proteins',\n",
       "  'REFERENCE': 'https://escholarship.org/uc/item/9gf6v85h (2020)'},\n",
       " 'P19515-8.0': {'MUTATION': ['E324R',\n",
       "   'E324M',\n",
       "   'E324F',\n",
       "   'E324Y',\n",
       "   'T116I',\n",
       "   'E324P',\n",
       "   'E324W',\n",
       "   'E324C',\n",
       "   'E324T',\n",
       "   'T112K',\n",
       "   'E324L',\n",
       "   'E324V',\n",
       "   'E324I',\n",
       "   'N280W',\n",
       "   'N358W',\n",
       "   'T112W',\n",
       "   'D332L',\n",
       "   'D332R',\n",
       "   'T112I',\n",
       "   'D332F',\n",
       "   'E314I',\n",
       "   'D320L',\n",
       "   'D332P',\n",
       "   'D332W',\n",
       "   'D332Y',\n",
       "   'E314V',\n",
       "   'S208Y',\n",
       "   'S208V',\n",
       "   'S208F',\n",
       "   'N214L',\n",
       "   'A102L',\n",
       "   'N280L',\n",
       "   'N321L',\n",
       "   'N214R',\n",
       "   'S208W',\n",
       "   'Q327I'],\n",
       "  '∆Tm': [1.2,\n",
       "   1.3,\n",
       "   1.6,\n",
       "   2.1,\n",
       "   2.4,\n",
       "   2.5,\n",
       "   2.7,\n",
       "   3.3,\n",
       "   3.8,\n",
       "   4.5,\n",
       "   5.4,\n",
       "   5.4,\n",
       "   5.7,\n",
       "   -3.1,\n",
       "   -2.7,\n",
       "   -2.2,\n",
       "   -2.5,\n",
       "   -2.8,\n",
       "   -3.0,\n",
       "   -3.0,\n",
       "   -2.7,\n",
       "   -3.3,\n",
       "   -3.8,\n",
       "   -4.5,\n",
       "   -5.4,\n",
       "   -5.3,\n",
       "   -5.5,\n",
       "   -4.9,\n",
       "   -6.4,\n",
       "   -7.5,\n",
       "   -8.8,\n",
       "   -10.0,\n",
       "   -13.0,\n",
       "   -13.6,\n",
       "   3.9,\n",
       "   2.3],\n",
       "  'UniProt_ID': 'P19515',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 8.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': '-',\n",
       "  'PubMed_ID': '19533118',\n",
       "  'KEY_WORDS': 'thermostability; Rhizomucor; yeast; lipase ',\n",
       "  'REFERENCE': 'Appl Microbiol Biotechnol 85, 117-26 (2009) PMID: 19533118'},\n",
       " 'P00974-2.0': {'MUTATION': ['K50W',\n",
       "   'K50H',\n",
       "   'K50R',\n",
       "   'K50D',\n",
       "   'K50F',\n",
       "   'K50M',\n",
       "   'K50T',\n",
       "   'F57A',\n",
       "   'Y58A',\n",
       "   'Y70G',\n",
       "   'N78G',\n",
       "   'F80A',\n",
       "   'G72A',\n",
       "   'N79G'],\n",
       "  '∆Tm': [4.0,\n",
       "   11.1,\n",
       "   8.8,\n",
       "   11.0,\n",
       "   5.9,\n",
       "   8.4,\n",
       "   9.7,\n",
       "   -9.0,\n",
       "   -33.0,\n",
       "   -18.0,\n",
       "   -32.0,\n",
       "   -38.0,\n",
       "   -21.0,\n",
       "   -21.0],\n",
       "  'UniProt_ID': 'P00974',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 2.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': '-',\n",
       "  'PubMed_ID': '11274462',\n",
       "  'KEY_WORDS': 'solvent-exposed residue; reverse hydrophobic effect; bovine pancreatic trypsin inhibitor',\n",
       "  'REFERENCE': 'Protein Sci 10, 715-24 (2001) PMID: 11274462'},\n",
       " 'P0A877-7.2': {'MUTATION': ['P28S',\n",
       "   'S33L',\n",
       "   'M101V',\n",
       "   'M101T',\n",
       "   'M101I',\n",
       "   'T24M',\n",
       "   'P78L',\n",
       "   'Y102C',\n",
       "   'Y102H',\n",
       "   'F22S',\n",
       "   'Q65R',\n",
       "   'P62Q',\n",
       "   'P78S',\n",
       "   'E49G',\n",
       "   'D56G',\n",
       "   'Y115C',\n",
       "   'D60G',\n",
       "   'A67V',\n",
       "   'D112N',\n",
       "   'F114L',\n",
       "   'P53H',\n",
       "   'T63K',\n",
       "   'P53T',\n",
       "   'P21S',\n",
       "   'G51D',\n",
       "   'P28L',\n",
       "   'D112G',\n",
       "   'S6P'],\n",
       "  '∆Tm': [-6.4,\n",
       "   -7.6,\n",
       "   7.6,\n",
       "   7.6,\n",
       "   3.7,\n",
       "   2.3,\n",
       "   1.9,\n",
       "   1.4,\n",
       "   0.8,\n",
       "   0.8,\n",
       "   -0.6,\n",
       "   -0.6,\n",
       "   -1.6,\n",
       "   -1.8,\n",
       "   -2.0,\n",
       "   -2.3,\n",
       "   -2.6,\n",
       "   -2.7,\n",
       "   -3.1,\n",
       "   -3.3,\n",
       "   -3.3,\n",
       "   -3.3,\n",
       "   -4.3,\n",
       "   -4.3,\n",
       "   -4.5,\n",
       "   -4.6,\n",
       "   -5.1,\n",
       "   -5.2],\n",
       "  'UniProt_ID': 'P0A877',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 30.3,\n",
       "  'pH': 7.2,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1727648',\n",
       "  'KEY_WORDS': 'tryptophan synthase alpha subunit; thermodynamic stability;',\n",
       "  'REFERENCE': 'ARCH BIOCHEM BIOPHYS 292, 34-41 (1992) PMID: 1727648'},\n",
       " 'P00651-6.0': {'MUTATION': ['W85Y',\n",
       "   'Y50W',\n",
       "   'Y68W',\n",
       "   'Y71W',\n",
       "   'H66T',\n",
       "   'H118A',\n",
       "   'F72L',\n",
       "   'F72V',\n",
       "   'F72A',\n",
       "   'Q51K',\n",
       "   'P119G',\n",
       "   'P140G',\n",
       "   'N93D',\n",
       "   'G114R'],\n",
       "  '∆Tm': [-2.9,\n",
       "   1.6,\n",
       "   -0.6,\n",
       "   -1.3,\n",
       "   -0.5,\n",
       "   -1.3,\n",
       "   -11.1,\n",
       "   -12.1,\n",
       "   -22.4,\n",
       "   2.8,\n",
       "   -6.2,\n",
       "   -9.5,\n",
       "   -0.2,\n",
       "   0.8],\n",
       "  'UniProt_ID': 'P00651',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 1.66,\n",
       "  'pH': 6.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8125111',\n",
       "  'KEY_WORDS': 'alfa-helix; active-site geometry; inhibitor conformation;',\n",
       "  'REFERENCE': 'EUR J BIOCHEM 220, 527-534 (1994) PMID: 8125111'},\n",
       " 'P00720-3.01': {'MUTATION': ['L46A',\n",
       "   'L118A',\n",
       "   'L121A',\n",
       "   'L99A',\n",
       "   'L133A',\n",
       "   'F153A',\n",
       "   'T115A',\n",
       "   'N116A',\n",
       "   'S117A',\n",
       "   'R119A',\n",
       "   'M120A',\n",
       "   'Q122A',\n",
       "   'Q123A',\n",
       "   'M102L',\n",
       "   'V111F',\n",
       "   'V111I',\n",
       "   'S44A',\n",
       "   'S44L',\n",
       "   'S44M',\n",
       "   'S44I',\n",
       "   'S44Q',\n",
       "   'S44R',\n",
       "   'S44K',\n",
       "   'S44Y',\n",
       "   'S44V',\n",
       "   'S44F',\n",
       "   'S44W',\n",
       "   'S44H',\n",
       "   'S44T',\n",
       "   'S44E',\n",
       "   'S44D',\n",
       "   'S44C',\n",
       "   'S44N',\n",
       "   'S44G',\n",
       "   'S44P',\n",
       "   'V131A',\n",
       "   'V131L',\n",
       "   'V131M',\n",
       "   'V131I',\n",
       "   'V131T',\n",
       "   'V131E',\n",
       "   'V131S',\n",
       "   'V131D',\n",
       "   'V131G',\n",
       "   'L99V',\n",
       "   'L99I',\n",
       "   'L99M',\n",
       "   'L99F',\n",
       "   'F153V',\n",
       "   'F153I',\n",
       "   'F153L',\n",
       "   'F153M'],\n",
       "  '∆Tm': [-8.6,\n",
       "   -12.2,\n",
       "   -9.3,\n",
       "   -15.7,\n",
       "   -8.9,\n",
       "   -12.3,\n",
       "   -0.43,\n",
       "   0.49,\n",
       "   3.64,\n",
       "   -0.53,\n",
       "   -0.58,\n",
       "   -0.71,\n",
       "   -0.64,\n",
       "   -2.54,\n",
       "   -4.77,\n",
       "   -2.32,\n",
       "   1.21,\n",
       "   1.09,\n",
       "   0.92,\n",
       "   0.91,\n",
       "   0.75,\n",
       "   0.68,\n",
       "   0.57,\n",
       "   0.54,\n",
       "   0.29,\n",
       "   0.18,\n",
       "   0.15,\n",
       "   0.12,\n",
       "   0.03,\n",
       "   0.0,\n",
       "   -0.32,\n",
       "   -0.35,\n",
       "   -0.4,\n",
       "   -1.55,\n",
       "   -10.32,\n",
       "   0.66,\n",
       "   0.23,\n",
       "   0.32,\n",
       "   0.41,\n",
       "   -0.33,\n",
       "   0.52,\n",
       "   -0.12,\n",
       "   0.22,\n",
       "   -1.8,\n",
       "   -6.6,\n",
       "   -4.0,\n",
       "   -2.0,\n",
       "   -1.1,\n",
       "   -6.1,\n",
       "   -1.5,\n",
       "   0.6,\n",
       "   -2.4],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 3.01,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1553543',\n",
       "  'KEY_WORDS': 'protein structure; hydrophobic effect; T4 lysozyme;',\n",
       "  'REFERENCE': 'SCIENCE 255, 178-183 (1992) PMID: 1553543'},\n",
       " 'P00651-5.0': {'MUTATION': ['K27W',\n",
       "   'F34W',\n",
       "   'D64W',\n",
       "   'Y99W',\n",
       "   'Y102W',\n",
       "   'Y118W',\n",
       "   'K130W',\n",
       "   'Y141W',\n",
       "   'V150W',\n",
       "   'V73G',\n",
       "   'R32A',\n",
       "   'K34A',\n",
       "   'Q37E',\n",
       "   'S45N',\n",
       "   'D44G',\n",
       "   'V73A',\n",
       "   'V80A',\n",
       "   'V80G',\n",
       "   'V83A',\n",
       "   'V83G',\n",
       "   'V89A',\n",
       "   'V89G',\n",
       "   'A90G',\n",
       "   'I107V',\n",
       "   'I107A',\n",
       "   'I107G',\n",
       "   'I132V',\n",
       "   'I132A',\n",
       "   'I133V',\n",
       "   'I133A',\n",
       "   'V134A',\n",
       "   'V134G',\n",
       "   'A135G',\n",
       "   'V142A',\n",
       "   'V142G',\n",
       "   'V144A',\n",
       "   'V144G',\n",
       "   'I132L',\n",
       "   'I133L',\n",
       "   'V80L',\n",
       "   'V80I',\n",
       "   'V83L',\n",
       "   'V83I',\n",
       "   'V134L',\n",
       "   'V134I',\n",
       "   'Q51K',\n",
       "   'Y123F',\n",
       "   'Y123A',\n",
       "   'Y123G',\n",
       "   'P68A',\n",
       "   'P119A',\n",
       "   'P140A',\n",
       "   'P143A',\n",
       "   'N93D'],\n",
       "  '∆Tm': [3.3,\n",
       "   -8.9,\n",
       "   -3.5,\n",
       "   -2.5,\n",
       "   -6.1,\n",
       "   1.1,\n",
       "   -10.9,\n",
       "   -4.4,\n",
       "   -4.5,\n",
       "   -26.8,\n",
       "   -2.7,\n",
       "   -2.2,\n",
       "   -0.8,\n",
       "   0.5,\n",
       "   -0.7,\n",
       "   -13.0,\n",
       "   -8.2,\n",
       "   -17.2,\n",
       "   -9.3,\n",
       "   -19.9,\n",
       "   0.1,\n",
       "   -11.9,\n",
       "   -2.2,\n",
       "   -0.7,\n",
       "   -10.3,\n",
       "   -17.0,\n",
       "   -3.0,\n",
       "   -14.9,\n",
       "   0.6,\n",
       "   -10.3,\n",
       "   -14.7,\n",
       "   -28.6,\n",
       "   -2.6,\n",
       "   -3.2,\n",
       "   -4.4,\n",
       "   -5.5,\n",
       "   -10.1,\n",
       "   -6.3,\n",
       "   -8.6,\n",
       "   -3.3,\n",
       "   -2.6,\n",
       "   -7.3,\n",
       "   -0.2,\n",
       "   -2.4,\n",
       "   0.1,\n",
       "   2.4,\n",
       "   -10.1,\n",
       "   -34.3,\n",
       "   -33.3,\n",
       "   -1.4,\n",
       "   -10.7,\n",
       "   -10.6,\n",
       "   -6.7,\n",
       "   0.2],\n",
       "  'UniProt_ID': 'P00651',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 123.48,\n",
       "  'pH': 5.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '11141060',\n",
       "  'KEY_WORDS': 'local unfolding; enzymatic activity; tertiary structure;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 40, 93-104 (2001) PMID: 11141060'},\n",
       " 'P00720-3.0': {'MUTATION': ['N40A',\n",
       "   'K43A',\n",
       "   'S44A',\n",
       "   'E45A',\n",
       "   'L46A',\n",
       "   'D47A',\n",
       "   'K48A',\n",
       "   'E128K',\n",
       "   'R96H',\n",
       "   'M102V',\n",
       "   'A146T',\n",
       "   'S117F',\n",
       "   'A41S',\n",
       "   'A42S',\n",
       "   'A49S',\n",
       "   'A73S',\n",
       "   'A82S',\n",
       "   'A93S',\n",
       "   'A98S',\n",
       "   'A130S',\n",
       "   'A134S',\n",
       "   'V75T',\n",
       "   'V87T',\n",
       "   'V149T',\n",
       "   'M102K',\n",
       "   'L133D',\n",
       "   'A98V',\n",
       "   'M102T',\n",
       "   'G156D',\n",
       "   'T157I',\n",
       "   'I3E',\n",
       "   'I3F',\n",
       "   'I3L',\n",
       "   'I3P',\n",
       "   'I3T',\n",
       "   'S38D',\n",
       "   'A82P',\n",
       "   'N144D',\n",
       "   'V131A',\n",
       "   'A41V',\n",
       "   'N116D',\n",
       "   'T152S',\n",
       "   'V149C',\n",
       "   'S38A',\n",
       "   'I17A',\n",
       "   'I27A',\n",
       "   'I29A',\n",
       "   'I50A',\n",
       "   'I58A',\n",
       "   'I78A',\n",
       "   'I100A',\n",
       "   'V71A',\n",
       "   'V87A',\n",
       "   'V94A',\n",
       "   'V103A',\n",
       "   'V111A',\n",
       "   'V149A',\n",
       "   'M6A',\n",
       "   'M106A',\n",
       "   'F67A',\n",
       "   'F104A',\n",
       "   'L7A',\n",
       "   'L33A',\n",
       "   'L66A',\n",
       "   'L84A',\n",
       "   'L91A',\n",
       "   'M6L',\n",
       "   'M106L',\n",
       "   'M120L',\n",
       "   'M106K',\n",
       "   'M120K',\n",
       "   'T115E',\n",
       "   'A42V',\n",
       "   'A42L',\n",
       "   'A42I',\n",
       "   'A42F',\n",
       "   'A98C',\n",
       "   'A98L',\n",
       "   'A98I',\n",
       "   'A98M',\n",
       "   'A98F',\n",
       "   'A98W',\n",
       "   'A129L',\n",
       "   'A129M',\n",
       "   'A129F',\n",
       "   'A129W',\n",
       "   'E108V',\n",
       "   'L99A',\n",
       "   'L99G',\n",
       "   'A42K'],\n",
       "  '∆Tm': [1.2,\n",
       "   -2.95,\n",
       "   1.2,\n",
       "   1.47,\n",
       "   -8.39,\n",
       "   -0.81,\n",
       "   -0.93,\n",
       "   -5.0,\n",
       "   -14.0,\n",
       "   -13.0,\n",
       "   -9.0,\n",
       "   4.8,\n",
       "   -1.77,\n",
       "   -7.49,\n",
       "   -1.53,\n",
       "   -1.27,\n",
       "   -0.99,\n",
       "   -0.52,\n",
       "   -7.47,\n",
       "   -2.89,\n",
       "   -0.44,\n",
       "   -3.7,\n",
       "   -4.55,\n",
       "   -10.08,\n",
       "   -35.0,\n",
       "   -14.9,\n",
       "   -13.0,\n",
       "   -13.0,\n",
       "   -5.0,\n",
       "   -11.0,\n",
       "   -3.66,\n",
       "   -1.31,\n",
       "   2.84,\n",
       "   -6.41,\n",
       "   -4.32,\n",
       "   0.46,\n",
       "   1.08,\n",
       "   0.94,\n",
       "   0.72,\n",
       "   1.1,\n",
       "   0.9,\n",
       "   -6.6,\n",
       "   -5.1,\n",
       "   -1.95,\n",
       "   -8.4,\n",
       "   -10.1,\n",
       "   -8.2,\n",
       "   -5.8,\n",
       "   -10.4,\n",
       "   -4.7,\n",
       "   -10.7,\n",
       "   -4.7,\n",
       "   -4.9,\n",
       "   -5.0,\n",
       "   -6.6,\n",
       "   -3.7,\n",
       "   -11.0,\n",
       "   -5.7,\n",
       "   -7.1,\n",
       "   -5.7,\n",
       "   -9.7,\n",
       "   -8.1,\n",
       "   -12.3,\n",
       "   -13.4,\n",
       "   -13.4,\n",
       "   -9.7,\n",
       "   -10.6,\n",
       "   1.7,\n",
       "   1.7,\n",
       "   -10.5,\n",
       "   -4.8,\n",
       "   -0.6,\n",
       "   -7.5,\n",
       "   -10.1,\n",
       "   -8.9,\n",
       "   -11.2,\n",
       "   -2.6,\n",
       "   -13.9,\n",
       "   -16.1,\n",
       "   -9.3,\n",
       "   -20.4,\n",
       "   -19.3,\n",
       "   -2.9,\n",
       "   -5.5,\n",
       "   -2.3,\n",
       "   -5.1,\n",
       "   4.3,\n",
       "   -15.6,\n",
       "   -29.2,\n",
       "   -15.3],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 86.75,\n",
       "  'pH': 3.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1570293',\n",
       "  'KEY_WORDS': 'alpha helix; protein folding; bacteriophage T4;',\n",
       "  'REFERENCE': 'PROC NATL ACAD SCI U S A 89, 3751-3755 (1992) PMID: 1570293'},\n",
       " 'P00720-6.7': {'MUTATION': ['N40A',\n",
       "   'K43A',\n",
       "   'S44A',\n",
       "   'E45A',\n",
       "   'L46A',\n",
       "   'D47A',\n",
       "   'K48A',\n",
       "   'S38N',\n",
       "   'D92N',\n",
       "   'T109D',\n",
       "   'T109N',\n",
       "   'N144H'],\n",
       "  '∆Tm': [0.88,\n",
       "   -3.08,\n",
       "   0.98,\n",
       "   0.04,\n",
       "   -6.4,\n",
       "   -2.75,\n",
       "   -1.68,\n",
       "   -0.1,\n",
       "   -3.7,\n",
       "   1.5,\n",
       "   0.3,\n",
       "   0.7],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 86.75,\n",
       "  'pH': 6.7,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1570293',\n",
       "  'KEY_WORDS': 'alpha helix; protein folding; bacteriophage T4;',\n",
       "  'REFERENCE': 'PROC NATL ACAD SCI U S A 89, 3751-3755 (1992) PMID: 1570293'},\n",
       " 'P00720-5.4': {'MUTATION': ['R14K',\n",
       "   'E22K',\n",
       "   'T26S',\n",
       "   'N40D',\n",
       "   'A41D',\n",
       "   'R80K',\n",
       "   'A93T',\n",
       "   'G113E',\n",
       "   'R119H',\n",
       "   'T151S',\n",
       "   'F153L',\n",
       "   'N163D',\n",
       "   'L121A',\n",
       "   'A129L',\n",
       "   'A129M',\n",
       "   'F153A',\n",
       "   'I58T',\n",
       "   'N101A',\n",
       "   'Q105M',\n",
       "   'V149A',\n",
       "   'V149S',\n",
       "   'V149C',\n",
       "   'V149T',\n",
       "   'V149I',\n",
       "   'V149G',\n",
       "   'T152A',\n",
       "   'T152S',\n",
       "   'T152C',\n",
       "   'T152V',\n",
       "   'T152I',\n",
       "   'S117F',\n",
       "   'S38D',\n",
       "   'A82P',\n",
       "   'N144D',\n",
       "   'I3L',\n",
       "   'V131A',\n",
       "   'A41V',\n",
       "   'N116D',\n",
       "   'E11F',\n",
       "   'E11M',\n",
       "   'E11A',\n",
       "   'G30A',\n",
       "   'G30F',\n",
       "   'S117V',\n",
       "   'S117I',\n",
       "   'N132M',\n",
       "   'N132F',\n",
       "   'N132I',\n",
       "   'E11H',\n",
       "   'E11N',\n",
       "   'D20N',\n",
       "   'D20T',\n",
       "   'D20S',\n",
       "   'D20A',\n",
       "   'M6A',\n",
       "   'L7A',\n",
       "   'I17A',\n",
       "   'L33A',\n",
       "   'I50A',\n",
       "   'L66A',\n",
       "   'I78A',\n",
       "   'L84A',\n",
       "   'V87A',\n",
       "   'L91A',\n",
       "   'L99A',\n",
       "   'M102A',\n",
       "   'I100A',\n",
       "   'V103A',\n",
       "   'F104A',\n",
       "   'M106A',\n",
       "   'V111A',\n",
       "   'L118A',\n",
       "   'I17M',\n",
       "   'I27M',\n",
       "   'L33M',\n",
       "   'I50M',\n",
       "   'L66M',\n",
       "   'V87M',\n",
       "   'I100M',\n",
       "   'F104M',\n",
       "   'V111M',\n",
       "   'V149M',\n",
       "   'E108V',\n",
       "   'L99G'],\n",
       "  '∆Tm': [-0.08,\n",
       "   1.37,\n",
       "   1.35,\n",
       "   1.14,\n",
       "   0.71,\n",
       "   -0.43,\n",
       "   0.13,\n",
       "   0.79,\n",
       "   -0.74,\n",
       "   0.93,\n",
       "   0.88,\n",
       "   -0.5,\n",
       "   -6.1,\n",
       "   -3.2,\n",
       "   -5.2,\n",
       "   -9.3,\n",
       "   -10.1,\n",
       "   -3.7,\n",
       "   -2.7,\n",
       "   -8.9,\n",
       "   -13.2,\n",
       "   -5.5,\n",
       "   -8.3,\n",
       "   -0.05,\n",
       "   -15.3,\n",
       "   -4.0,\n",
       "   -5.5,\n",
       "   -1.4,\n",
       "   0.8,\n",
       "   -1.0,\n",
       "   2.8,\n",
       "   1.36,\n",
       "   1.34,\n",
       "   0.99,\n",
       "   1.69,\n",
       "   0.87,\n",
       "   0.7,\n",
       "   1.36,\n",
       "   4.3,\n",
       "   4.1,\n",
       "   2.6,\n",
       "   0.1,\n",
       "   -4.9,\n",
       "   5.1,\n",
       "   4.2,\n",
       "   3.6,\n",
       "   3.3,\n",
       "   3.0,\n",
       "   0.1,\n",
       "   -0.6,\n",
       "   3.1,\n",
       "   2.2,\n",
       "   1.6,\n",
       "   -0.8,\n",
       "   -4.5,\n",
       "   -6.3,\n",
       "   -6.4,\n",
       "   -8.8,\n",
       "   -4.2,\n",
       "   -10.1,\n",
       "   -3.5,\n",
       "   -10.5,\n",
       "   -4.3,\n",
       "   -7.4,\n",
       "   -11.7,\n",
       "   -8.2,\n",
       "   -7.1,\n",
       "   -4.4,\n",
       "   -7.5,\n",
       "   -5.2,\n",
       "   -2.9,\n",
       "   -0.9,\n",
       "   -5.9,\n",
       "   -10.1,\n",
       "   -5.3,\n",
       "   -0.6,\n",
       "   -2.5,\n",
       "   -6.3,\n",
       "   -4.5,\n",
       "   -0.8,\n",
       "   -2.0,\n",
       "   -7.8,\n",
       "   2.4,\n",
       "   -20.3],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 54.59,\n",
       "  'pH': 5.4,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '7507755',\n",
       "  'KEY_WORDS': 'protein stability; screening;mutagenesis',\n",
       "  'REFERENCE': 'PROTEIN SCI 2, 2217-2225 (1993) PMID: 7507755'},\n",
       " 'P00720-6.78': {'MUTATION': ['R14K',\n",
       "   'E22K',\n",
       "   'T26S',\n",
       "   'N40D',\n",
       "   'A41D',\n",
       "   'R80K',\n",
       "   'A93T',\n",
       "   'G113E',\n",
       "   'R119H',\n",
       "   'T151S',\n",
       "   'F153L',\n",
       "   'N163D'],\n",
       "  '∆Tm': [-0.53,\n",
       "   1.58,\n",
       "   1.35,\n",
       "   1.28,\n",
       "   1.1,\n",
       "   -0.34,\n",
       "   0.16,\n",
       "   1.03,\n",
       "   -0.83,\n",
       "   0.97,\n",
       "   0.51,\n",
       "   -0.49],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 54.59,\n",
       "  'pH': 6.78,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '7507755',\n",
       "  'KEY_WORDS': 'protein stability; screening;mutagenesis',\n",
       "  'REFERENCE': 'PROTEIN SCI 2, 2217-2225 (1993) PMID: 7507755'},\n",
       " 'P00644-7.0': {'MUTATION': ['L107A',\n",
       "   'V148L',\n",
       "   'G161S',\n",
       "   'G170V',\n",
       "   'A172S',\n",
       "   'H206L',\n",
       "   'E155G',\n",
       "   'D159G',\n",
       "   'S223A',\n",
       "   'W222F',\n",
       "   'W222Y',\n",
       "   'W222H',\n",
       "   'V105T',\n",
       "   'V121T',\n",
       "   'V133T',\n",
       "   'V148T',\n",
       "   'V156T',\n",
       "   'V181T',\n",
       "   'V186T',\n",
       "   'V193T',\n",
       "   'V196T',\n",
       "   'V121S',\n",
       "   'V133S',\n",
       "   'V148S',\n",
       "   'V196S',\n",
       "   'Y109F',\n",
       "   'Y136F',\n",
       "   'Y167F',\n",
       "   'Y173F',\n",
       "   'Y175F',\n",
       "   'Y195F',\n",
       "   'Y197F',\n",
       "   'Y136L',\n",
       "   'Y167L',\n",
       "   'Y173L',\n",
       "   'Y195L',\n",
       "   'Y197L',\n",
       "   'S210A',\n",
       "   'S229A',\n",
       "   'T95S',\n",
       "   'T104S',\n",
       "   'T115S',\n",
       "   'T123S',\n",
       "   'T126S',\n",
       "   'T144S',\n",
       "   'T164S',\n",
       "   'T202S',\n",
       "   'T95V',\n",
       "   'T104V',\n",
       "   'T115V',\n",
       "   'T123V',\n",
       "   'T126V',\n",
       "   'T144V',\n",
       "   'T164V',\n",
       "   'T202V',\n",
       "   'T95C',\n",
       "   'T104C',\n",
       "   'T115C',\n",
       "   'T123C',\n",
       "   'T126C',\n",
       "   'T144C',\n",
       "   'T164C',\n",
       "   'T202C',\n",
       "   'T104I',\n",
       "   'T115I',\n",
       "   'T123I',\n",
       "   'T126I',\n",
       "   'T144I',\n",
       "   'T164I',\n",
       "   'T202I',\n",
       "   'D101F',\n",
       "   'K110F',\n",
       "   'G111F',\n",
       "   'P129F',\n",
       "   'K130F',\n",
       "   'K131F',\n",
       "   'E134F',\n",
       "   'E139F',\n",
       "   'M147F',\n",
       "   'E149F',\n",
       "   'E155F',\n",
       "   'Q162F',\n",
       "   'K166F',\n",
       "   'G168F',\n",
       "   'G178F',\n",
       "   'E183F',\n",
       "   'A194F',\n",
       "   'K198F',\n",
       "   'E204F',\n",
       "   'Q205F',\n",
       "   'K209F',\n",
       "   'S210F',\n",
       "   'E217F',\n",
       "   'K218F',\n",
       "   'D225F',\n",
       "   'T144A',\n",
       "   'T144F',\n",
       "   'T144G',\n",
       "   'T144H',\n",
       "   'T144K',\n",
       "   'T144L',\n",
       "   'T144M',\n",
       "   'T144N',\n",
       "   'T144Q',\n",
       "   'V148W',\n",
       "   'K152W',\n",
       "   'G170W',\n",
       "   'V148A',\n",
       "   'L89A',\n",
       "   'V105A',\n",
       "   'K106G',\n",
       "   'A151T',\n",
       "   'E157A',\n",
       "   'E157G',\n",
       "   'L219A',\n",
       "   'D159A',\n",
       "   'E157V',\n",
       "   'P199G',\n",
       "   'P199T',\n",
       "   'K198G',\n",
       "   'M114L',\n",
       "   'M114I',\n",
       "   'G111C',\n",
       "   'G132C',\n",
       "   'E139C',\n",
       "   'A142C',\n",
       "   'K152C',\n",
       "   'K160C',\n",
       "   'R187C',\n",
       "   'A194C',\n",
       "   'K216C',\n",
       "   'G132F',\n",
       "   'K152F',\n",
       "   'K160F',\n",
       "   'R187F',\n",
       "   'K216F'],\n",
       "  '∆Tm': [-11.5,\n",
       "   3.9,\n",
       "   -7.6,\n",
       "   2.9,\n",
       "   -10.7,\n",
       "   5.1,\n",
       "   -15.2,\n",
       "   -8.1,\n",
       "   -0.8,\n",
       "   -11.6,\n",
       "   -12.1,\n",
       "   -8.9,\n",
       "   -14.7,\n",
       "   -7.5,\n",
       "   -1.0,\n",
       "   -6.3,\n",
       "   -18.3,\n",
       "   -15.2,\n",
       "   -10.3,\n",
       "   -10.1,\n",
       "   -1.0,\n",
       "   -11.0,\n",
       "   -0.6,\n",
       "   -13.9,\n",
       "   -0.5,\n",
       "   -3.9,\n",
       "   -1.8,\n",
       "   -1.3,\n",
       "   -7.2,\n",
       "   -7.8,\n",
       "   -0.4,\n",
       "   -0.5,\n",
       "   -12.4,\n",
       "   -0.7,\n",
       "   -14.1,\n",
       "   -0.7,\n",
       "   -1.4,\n",
       "   3.0,\n",
       "   -0.6,\n",
       "   -1.6,\n",
       "   -2.8,\n",
       "   -5.5,\n",
       "   -3.1,\n",
       "   -0.5,\n",
       "   -9.0,\n",
       "   -3.5,\n",
       "   -2.8,\n",
       "   -1.6,\n",
       "   -3.6,\n",
       "   0.6,\n",
       "   2.7,\n",
       "   -0.5,\n",
       "   2.2,\n",
       "   0.7,\n",
       "   -5.4,\n",
       "   -3.8,\n",
       "   -3.8,\n",
       "   -3.6,\n",
       "   1.3,\n",
       "   -0.3,\n",
       "   -4.6,\n",
       "   -2.5,\n",
       "   -5.3,\n",
       "   -3.0,\n",
       "   1.1,\n",
       "   4.0,\n",
       "   -4.2,\n",
       "   -3.9,\n",
       "   0.8,\n",
       "   -5.0,\n",
       "   -4.6,\n",
       "   -4.2,\n",
       "   -6.4,\n",
       "   -0.9,\n",
       "   -0.4,\n",
       "   -1.2,\n",
       "   -0.5,\n",
       "   -1.1,\n",
       "   -5.8,\n",
       "   -6.6,\n",
       "   -5.3,\n",
       "   -1.6,\n",
       "   -3.5,\n",
       "   -9.1,\n",
       "   -10.7,\n",
       "   -16.1,\n",
       "   -6.9,\n",
       "   0.2,\n",
       "   -1.0,\n",
       "   -2.0,\n",
       "   -1.2,\n",
       "   -3.8,\n",
       "   -3.5,\n",
       "   -6.4,\n",
       "   -1.5,\n",
       "   -7.0,\n",
       "   0.5,\n",
       "   -15.8,\n",
       "   -8.2,\n",
       "   -17.5,\n",
       "   -4.4,\n",
       "   -1.3,\n",
       "   -16.7,\n",
       "   -13.5,\n",
       "   6.1,\n",
       "   -3.4,\n",
       "   4.8,\n",
       "   -11.9,\n",
       "   -3.2,\n",
       "   -12.5,\n",
       "   -6.3,\n",
       "   -13.7,\n",
       "   -5.6,\n",
       "   -15.2,\n",
       "   -8.7,\n",
       "   -10.7,\n",
       "   -6.6,\n",
       "   5.0,\n",
       "   0.6,\n",
       "   3.8,\n",
       "   -3.2,\n",
       "   -2.3,\n",
       "   -4.6,\n",
       "   -2.6,\n",
       "   -0.9,\n",
       "   -3.6,\n",
       "   -2.8,\n",
       "   0.4,\n",
       "   -11.1,\n",
       "   -2.8,\n",
       "   -2.9,\n",
       "   -2.5,\n",
       "   0.0,\n",
       "   -0.5,\n",
       "   -15.1,\n",
       "   -1.7],\n",
       "  'UniProt_ID': 'P00644',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8518730',\n",
       "  'KEY_WORDS': 'thermal unfolding; free energies of unfolding; differential scanning',\n",
       "  'REFERENCE': 'PROTEIN SCI 2, 567-576 (1993) PMID: 8518730'},\n",
       " 'P00720-2.0': {'MUTATION': ['W138Y',\n",
       "   'Q69P',\n",
       "   'D72P',\n",
       "   'G77A',\n",
       "   'A82P',\n",
       "   'T157N',\n",
       "   'T157S',\n",
       "   'T157D',\n",
       "   'T157G',\n",
       "   'T157C',\n",
       "   'T157L',\n",
       "   'T157R',\n",
       "   'T157A',\n",
       "   'T157E',\n",
       "   'T157V',\n",
       "   'T157H',\n",
       "   'T157F',\n",
       "   'T157I',\n",
       "   'N55G',\n",
       "   'K124G',\n",
       "   'I3W',\n",
       "   'I3Y',\n",
       "   'I3F',\n",
       "   'I3L',\n",
       "   'I3V',\n",
       "   'I3M',\n",
       "   'I3C',\n",
       "   'I3CBased on UniProt and PDB)',\n",
       "   'I3A',\n",
       "   'I3T',\n",
       "   'I3S',\n",
       "   'I3G',\n",
       "   'I3E',\n",
       "   'I3D',\n",
       "   'K60P',\n",
       "   'A93P',\n",
       "   'G113A',\n",
       "   'S38N',\n",
       "   'D92N',\n",
       "   'T109D',\n",
       "   'T109N',\n",
       "   'N144H',\n",
       "   'G156D',\n",
       "   'P86A',\n",
       "   'P86R',\n",
       "   'P86D',\n",
       "   'P86C',\n",
       "   'P86G',\n",
       "   'P86H',\n",
       "   'P86I',\n",
       "   'P86L',\n",
       "   'P86S',\n",
       "   'P86T',\n",
       "   'I3P',\n",
       "   'T59N',\n",
       "   'T59S',\n",
       "   'T59D',\n",
       "   'T59G',\n",
       "   'T59V',\n",
       "   'T59A',\n",
       "   'P37A',\n",
       "   'L39P',\n",
       "   'P143A',\n",
       "   'S38D',\n",
       "   'T115E',\n",
       "   'N116D',\n",
       "   'R119M',\n",
       "   'N144D',\n",
       "   'Q123E',\n",
       "   'N144E',\n",
       "   'K60H',\n",
       "   'S90H',\n",
       "   'K83H',\n",
       "   'R96H',\n",
       "   'E128A',\n",
       "   'V131A'],\n",
       "  '∆Tm': [-3.0,\n",
       "   -12.9,\n",
       "   -10.2,\n",
       "   -1.4,\n",
       "   0.8,\n",
       "   -1.7,\n",
       "   -2.5,\n",
       "   -4.2,\n",
       "   -4.2,\n",
       "   -4.9,\n",
       "   -5.0,\n",
       "   -5.1,\n",
       "   -5.4,\n",
       "   -5.8,\n",
       "   -6.0,\n",
       "   -7.9,\n",
       "   -9.2,\n",
       "   -11.0,\n",
       "   -1.7,\n",
       "   0.0,\n",
       "   -16.4,\n",
       "   -9.5,\n",
       "   -4.0,\n",
       "   3.0,\n",
       "   -2.1,\n",
       "   -0.9,\n",
       "   -1.9,\n",
       "   4.8,\n",
       "   -3.8,\n",
       "   -6.1,\n",
       "   -7.0,\n",
       "   -7.2,\n",
       "   -4.1,\n",
       "   -6.5,\n",
       "   0.3,\n",
       "   0.4,\n",
       "   0.4,\n",
       "   -0.3,\n",
       "   -0.3,\n",
       "   -0.8,\n",
       "   -0.1,\n",
       "   -2.3,\n",
       "   -5.1,\n",
       "   -2.0,\n",
       "   -2.0,\n",
       "   0.0,\n",
       "   -1.0,\n",
       "   -2.0,\n",
       "   -2.0,\n",
       "   -2.0,\n",
       "   -2.0,\n",
       "   -1.0,\n",
       "   -1.0,\n",
       "   -11.99,\n",
       "   -2.1,\n",
       "   -2.6,\n",
       "   -3.1,\n",
       "   -7.7,\n",
       "   -10.0,\n",
       "   -10.1,\n",
       "   -0.8,\n",
       "   -5.5,\n",
       "   -5.0,\n",
       "   -0.3,\n",
       "   -1.7,\n",
       "   -0.2,\n",
       "   -0.9,\n",
       "   -0.2,\n",
       "   1.0,\n",
       "   0.9,\n",
       "   -3.4,\n",
       "   -4.1,\n",
       "   -2.2,\n",
       "   -15.6,\n",
       "   0.6,\n",
       "   1.0],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 4.99,\n",
       "  'pH': 2.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '7306671',\n",
       "  'KEY_WORDS': 'point mutations; T4 lysozyme; protein structure;',\n",
       "  'REFERENCE': 'BIOPOLYMERS 20, 1989-1999 (1981); doi:10.1002/bip.1981.360200921\\xa0 PMID: 7306671'},\n",
       " 'P0A7Y4-5.5': {'MUTATION': ['D94E',\n",
       "   'K95G',\n",
       "   'K95A',\n",
       "   'K95N',\n",
       "   'A125T',\n",
       "   'H62P',\n",
       "   'Q113P',\n",
       "   'K91R',\n",
       "   'G23A',\n",
       "   'V74L',\n",
       "   'D134H',\n",
       "   'D134N',\n",
       "   'D134E',\n",
       "   'D134Q',\n",
       "   'D134S',\n",
       "   'D134T',\n",
       "   'D134V',\n",
       "   'D134I',\n",
       "   'D134L',\n",
       "   'D134A',\n",
       "   'I53A',\n",
       "   'Q105G',\n",
       "   'D10A',\n",
       "   'R27A',\n",
       "   'H83A',\n",
       "   'H124A',\n",
       "   'V74I',\n",
       "   'V74A',\n",
       "   'A24V',\n",
       "   'A52V',\n",
       "   'H62R',\n",
       "   'H62D',\n",
       "   'Q76L',\n",
       "   'Q80L',\n",
       "   'K117R',\n",
       "   'E119V',\n",
       "   'R41C',\n",
       "   'E135K'],\n",
       "  '∆Tm': [-1.6,\n",
       "   6.8,\n",
       "   0.4,\n",
       "   3.2,\n",
       "   0.0,\n",
       "   4.1,\n",
       "   -2.1,\n",
       "   0.1,\n",
       "   1.8,\n",
       "   3.3,\n",
       "   7.0,\n",
       "   3.2,\n",
       "   3.1,\n",
       "   4.8,\n",
       "   3.9,\n",
       "   3.9,\n",
       "   4.1,\n",
       "   4.6,\n",
       "   5.5,\n",
       "   5.5,\n",
       "   -12.1,\n",
       "   -9.6,\n",
       "   14.8,\n",
       "   -5.6,\n",
       "   0.1,\n",
       "   0.0,\n",
       "   2.1,\n",
       "   -12.7,\n",
       "   3.2,\n",
       "   7.8,\n",
       "   1.3,\n",
       "   -0.6,\n",
       "   1.1,\n",
       "   0.8,\n",
       "   0.1,\n",
       "   2.7,\n",
       "   1.6,\n",
       "   -0.8],\n",
       "  'UniProt_ID': 'P0A7Y4',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 76.34,\n",
       "  'pH': 5.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1331044',\n",
       "  'KEY_WORDS': 'structural stability; mutagenesis; free energy change;',\n",
       "  'REFERENCE': 'J BIOL CHEM 267, 22014-22017 (1992) PMID: 1331044'},\n",
       " 'P0A7Y4-3.0': {'MUTATION': ['K95G',\n",
       "   'K95A',\n",
       "   'K95N',\n",
       "   'D10N',\n",
       "   'D10A',\n",
       "   'D10E',\n",
       "   'D10S',\n",
       "   'D10H',\n",
       "   'E48Q',\n",
       "   'E48A',\n",
       "   'E48D',\n",
       "   'D70N',\n",
       "   'D70A',\n",
       "   'D70E',\n",
       "   'D134N',\n",
       "   'D134A',\n",
       "   'A125T',\n",
       "   'H62P',\n",
       "   'Q113P',\n",
       "   'S68G',\n",
       "   'S68A',\n",
       "   'S68T',\n",
       "   'S68V',\n",
       "   'S68L',\n",
       "   'K91R',\n",
       "   'D94E',\n",
       "   'G23A',\n",
       "   'V74L',\n",
       "   'D134H',\n",
       "   'D134E',\n",
       "   'D134Q',\n",
       "   'D134S',\n",
       "   'D134T',\n",
       "   'D134V',\n",
       "   'D134I',\n",
       "   'D134L',\n",
       "   'H62A',\n",
       "   'H83A',\n",
       "   'H114A',\n",
       "   'H124A',\n",
       "   'H127A',\n",
       "   'G77A',\n",
       "   'V74I',\n",
       "   'V74A',\n",
       "   'A24V',\n",
       "   'A52V',\n",
       "   'H62R',\n",
       "   'H62D',\n",
       "   'Q76L',\n",
       "   'Q80L',\n",
       "   'K117R',\n",
       "   'E119V',\n",
       "   'R41C',\n",
       "   'E135K'],\n",
       "  '∆Tm': [5.7,\n",
       "   0.3,\n",
       "   2.9,\n",
       "   -2.6,\n",
       "   8.1,\n",
       "   3.8,\n",
       "   2.4,\n",
       "   1.5,\n",
       "   -0.2,\n",
       "   -0.2,\n",
       "   0.8,\n",
       "   -1.2,\n",
       "   -0.3,\n",
       "   1.8,\n",
       "   -0.3,\n",
       "   2.9,\n",
       "   0.4,\n",
       "   3.4,\n",
       "   -0.6,\n",
       "   -8.0,\n",
       "   -1.5,\n",
       "   -0.7,\n",
       "   1.9,\n",
       "   -1.6,\n",
       "   0.5,\n",
       "   -1.2,\n",
       "   2.3,\n",
       "   3.7,\n",
       "   1.7,\n",
       "   2.4,\n",
       "   1.6,\n",
       "   0.9,\n",
       "   0.4,\n",
       "   1.1,\n",
       "   2.3,\n",
       "   3.8,\n",
       "   1.3,\n",
       "   2.1,\n",
       "   -2.3,\n",
       "   1.7,\n",
       "   0.9,\n",
       "   -2.9,\n",
       "   2.4,\n",
       "   -7.6,\n",
       "   2.4,\n",
       "   5.5,\n",
       "   -0.1,\n",
       "   2.2,\n",
       "   0.8,\n",
       "   1.0,\n",
       "   -0.2,\n",
       "   0.3,\n",
       "   0.4,\n",
       "   -3.7],\n",
       "  'UniProt_ID': 'P0A7Y4',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 68.06,\n",
       "  'pH': 3.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1331044',\n",
       "  'KEY_WORDS': 'structural stability; mutagenesis; free energy change;',\n",
       "  'REFERENCE': 'J BIOL CHEM 267, 22014-22017 (1992) PMID: 1331044'},\n",
       " 'P00720-5.42': {'MUTATION': ['I78M',\n",
       "   'L84M',\n",
       "   'L91M',\n",
       "   'L99M',\n",
       "   'I100M',\n",
       "   'V103M',\n",
       "   'L118M',\n",
       "   'L121M',\n",
       "   'L133M',\n",
       "   'F153M'],\n",
       "  '∆Tm': [-3.7, -4.9, -2.0, -1.3, -4.5, -3.1, -1.8, -2.1, -1.0, -1.6],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 5.42,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '8901549',\n",
       "  'KEY_WORDS': 'T4 lysozyme; protein folding; methionine substitutions;',\n",
       "  'REFERENCE': 'PROC NATL ACAD SCI U S A 93, 12155-12158 (1996) PMID: 8901549'},\n",
       " 'P61626-2.7': {'MUTATION': ['L26T',\n",
       "   'A27S',\n",
       "   'A50S',\n",
       "   'A110S',\n",
       "   'V111T',\n",
       "   'A114S',\n",
       "   'V117T',\n",
       "   'V118T',\n",
       "   'R39A',\n",
       "   'R39G',\n",
       "   'Y56A',\n",
       "   'Y56G',\n",
       "   'R68A',\n",
       "   'Q76A',\n",
       "   'H96A',\n",
       "   'N136A',\n",
       "   'N136G',\n",
       "   'G34A',\n",
       "   'G37A',\n",
       "   'G40A',\n",
       "   'G55A',\n",
       "   'G66A',\n",
       "   'G86A',\n",
       "   'G90A',\n",
       "   'G123A',\n",
       "   'G145A',\n",
       "   'G147A',\n",
       "   'V20G',\n",
       "   'V20I',\n",
       "   'V20L',\n",
       "   'V20M',\n",
       "   'V20F',\n",
       "   'V92G',\n",
       "   'V92I',\n",
       "   'V92L',\n",
       "   'V92M',\n",
       "   'V92F',\n",
       "   'V128G',\n",
       "   'V128I',\n",
       "   'V128L',\n",
       "   'V128M',\n",
       "   'V128F',\n",
       "   'V20S',\n",
       "   'V20Y',\n",
       "   'V20D',\n",
       "   'V20N',\n",
       "   'V20R',\n",
       "   'V92S',\n",
       "   'V92Y',\n",
       "   'V92D',\n",
       "   'V92N',\n",
       "   'V92R',\n",
       "   'V128Y',\n",
       "   'V128D',\n",
       "   'V128N',\n",
       "   'V128R',\n",
       "   'I77A',\n",
       "   'I41V',\n",
       "   'I74V',\n",
       "   'I77V',\n",
       "   'I107V',\n",
       "   'I124V',\n",
       "   'V20A',\n",
       "   'V92A',\n",
       "   'V111A',\n",
       "   'V117A',\n",
       "   'V118A',\n",
       "   'V128A',\n",
       "   'V139A',\n",
       "   'V143A',\n",
       "   'V148A',\n",
       "   'Y38F',\n",
       "   'Y56F',\n",
       "   'Y63F',\n",
       "   'Y72F',\n",
       "   'Y81F',\n",
       "   'Y142F',\n",
       "   'I41A',\n",
       "   'I74A',\n",
       "   'I107A',\n",
       "   'I124A',\n",
       "   'I77G',\n",
       "   'S42A',\n",
       "   'S54A',\n",
       "   'S69A',\n",
       "   'S79A',\n",
       "   'S98A',\n",
       "   'S100A',\n",
       "   'N45L',\n",
       "   'A50L',\n",
       "   'E53L',\n",
       "   'G55Q',\n",
       "   'R68G',\n",
       "   'Q76G',\n",
       "   'H96G',\n",
       "   'A114M',\n",
       "   'V118F',\n",
       "   'I74G',\n",
       "   'I74L',\n",
       "   'I74M',\n",
       "   'I74F',\n",
       "   'I74T',\n",
       "   'I77L',\n",
       "   'I77M',\n",
       "   'I77F',\n",
       "   'I77S',\n",
       "   'I77T',\n",
       "   'I77Y',\n",
       "   'K19M',\n",
       "   'K19A'],\n",
       "  '∆Tm': [-12.4,\n",
       "   -0.1,\n",
       "   -1.0,\n",
       "   2.6,\n",
       "   -2.3,\n",
       "   -3.3,\n",
       "   -1.6,\n",
       "   -0.9,\n",
       "   4.5,\n",
       "   3.7,\n",
       "   -8.9,\n",
       "   -8.2,\n",
       "   1.4,\n",
       "   3.1,\n",
       "   -0.5,\n",
       "   0.6,\n",
       "   0.2,\n",
       "   -4.6,\n",
       "   -5.5,\n",
       "   -7.2,\n",
       "   -0.9,\n",
       "   1.5,\n",
       "   -0.4,\n",
       "   -1.1,\n",
       "   -2.1,\n",
       "   -1.7,\n",
       "   0.5,\n",
       "   -7.5,\n",
       "   3.4,\n",
       "   -0.2,\n",
       "   -1.0,\n",
       "   -2.7,\n",
       "   -0.7,\n",
       "   1.7,\n",
       "   0.6,\n",
       "   2.0,\n",
       "   -0.9,\n",
       "   1.6,\n",
       "   2.7,\n",
       "   0.2,\n",
       "   1.6,\n",
       "   -0.2,\n",
       "   -4.4,\n",
       "   -1.2,\n",
       "   -4.5,\n",
       "   -4.1,\n",
       "   -1.3,\n",
       "   0.0,\n",
       "   -0.9,\n",
       "   -1.4,\n",
       "   -1.0,\n",
       "   -0.3,\n",
       "   -0.5,\n",
       "   0.5,\n",
       "   0.2,\n",
       "   2.9,\n",
       "   -5.8,\n",
       "   -1.6,\n",
       "   -6.3,\n",
       "   -4.4,\n",
       "   -2.7,\n",
       "   -4.7,\n",
       "   -7.9,\n",
       "   -1.9,\n",
       "   -4.4,\n",
       "   -4.4,\n",
       "   -2.6,\n",
       "   0.3,\n",
       "   -9.3,\n",
       "   -7.0,\n",
       "   -4.8,\n",
       "   -1.5,\n",
       "   -0.6,\n",
       "   0.2,\n",
       "   -3.0,\n",
       "   -0.7,\n",
       "   -1.1,\n",
       "   -8.1,\n",
       "   -12.5,\n",
       "   -8.7,\n",
       "   -3.0,\n",
       "   -12.7,\n",
       "   -1.6,\n",
       "   -3.5,\n",
       "   -0.7,\n",
       "   -4.3,\n",
       "   1.4,\n",
       "   1.1,\n",
       "   0.4,\n",
       "   -0.3,\n",
       "   -1.8,\n",
       "   -0.8,\n",
       "   0.9,\n",
       "   5.7,\n",
       "   -0.5,\n",
       "   0.1,\n",
       "   -6.5,\n",
       "   -2.7,\n",
       "   -0.3,\n",
       "   -5.8,\n",
       "   -15.0,\n",
       "   -12.5,\n",
       "   0.0,\n",
       "   -4.2,\n",
       "   -2.6,\n",
       "   -11.8,\n",
       "   -6.9,\n",
       "   -13.8,\n",
       "   -0.5,\n",
       "   -2.2],\n",
       "  'UniProt_ID': 'P61626',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 2.7,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '11294653',\n",
       "  'KEY_WORDS': 'buried polar groups; conformational stability; low-energy penalty;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 40 4853-4858 (2001) PMID: 11294653'},\n",
       " 'P00651-7.0': {'MUTATION': ['V42A',\n",
       "   'V42S',\n",
       "   'V42T',\n",
       "   'V42C',\n",
       "   'V104A',\n",
       "   'V104S',\n",
       "   'V104T',\n",
       "   'V115S',\n",
       "   'V115T',\n",
       "   'V115C',\n",
       "   'Q51K',\n",
       "   'E84A',\n",
       "   'Y37F',\n",
       "   'Y68F',\n",
       "   'Y82F',\n",
       "   'Y83F',\n",
       "   'Y94F',\n",
       "   'S38A',\n",
       "   'S43A',\n",
       "   'S90A',\n",
       "   'N35A',\n",
       "   'N62A',\n",
       "   'N70A',\n",
       "   'N107A',\n",
       "   'N70D',\n",
       "   'N70S',\n",
       "   'A30S',\n",
       "   'A31S',\n",
       "   'S149A',\n",
       "   'W85Y',\n",
       "   'Y71W',\n",
       "   'D102N',\n",
       "   'D102S',\n",
       "   'D102A',\n",
       "   'T117A',\n",
       "   'T117V',\n",
       "   'T119A',\n",
       "   'T119V'],\n",
       "  '∆Tm': [-8.2,\n",
       "   -15.6,\n",
       "   -11.3,\n",
       "   -9.6,\n",
       "   -7.9,\n",
       "   -16.6,\n",
       "   -9.8,\n",
       "   -21.6,\n",
       "   -13.2,\n",
       "   -4.9,\n",
       "   3.4,\n",
       "   -2.3,\n",
       "   -6.0,\n",
       "   3.4,\n",
       "   -2.1,\n",
       "   -1.3,\n",
       "   -4.0,\n",
       "   -3.2,\n",
       "   1.7,\n",
       "   -4.6,\n",
       "   -2.1,\n",
       "   0.0,\n",
       "   -5.5,\n",
       "   -8.6,\n",
       "   -5.5,\n",
       "   -5.0,\n",
       "   -1.54,\n",
       "   -1.11,\n",
       "   1.38,\n",
       "   -4.2,\n",
       "   -1.6,\n",
       "   -13.8,\n",
       "   -13.6,\n",
       "   -15.2,\n",
       "   -10.6,\n",
       "   -11.8,\n",
       "   -2.3,\n",
       "   -3.2],\n",
       "  'UniProt_ID': 'P00651',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 1.95,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes(0.98)',\n",
       "  'PubMed_ID': '11513591',\n",
       "  'KEY_WORDS': 'cavity; hydrophobic core; sulfhydryl group; hydroxyl; methyl groups',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 40 10140-10149 (2001) PMID: 11513591'},\n",
       " 'P00720-6.5': {'MUTATION': ['Q69P',\n",
       "   'D72P',\n",
       "   'A74P',\n",
       "   'G77A',\n",
       "   'A82P',\n",
       "   'L133D',\n",
       "   'N55G',\n",
       "   'K124G',\n",
       "   'I3W',\n",
       "   'I3Y',\n",
       "   'I3F',\n",
       "   'I3L',\n",
       "   'I3V',\n",
       "   'I3M',\n",
       "   'I3C',\n",
       "   'I3A',\n",
       "   'I3T',\n",
       "   'I3S',\n",
       "   'I3G',\n",
       "   'I3E',\n",
       "   'I3D',\n",
       "   'K60P',\n",
       "   'A93P',\n",
       "   'G113A',\n",
       "   'C54V',\n",
       "   'C54T',\n",
       "   'R96H',\n",
       "   'A146T',\n",
       "   'G156D',\n",
       "   'T59N',\n",
       "   'T59S',\n",
       "   'T59D',\n",
       "   'T59G',\n",
       "   'T59V',\n",
       "   'T59A',\n",
       "   'T115E',\n",
       "   'Q123E',\n",
       "   'N144E',\n",
       "   'K60H',\n",
       "   'S90H',\n",
       "   'K83H'],\n",
       "  '∆Tm': [-7.6,\n",
       "   -6.9,\n",
       "   -12.4,\n",
       "   0.94,\n",
       "   2.1,\n",
       "   -17.9,\n",
       "   -1.6,\n",
       "   -0.2,\n",
       "   -8.0,\n",
       "   -5.9,\n",
       "   -3.0,\n",
       "   0.9,\n",
       "   -1.2,\n",
       "   -2.3,\n",
       "   -3.7,\n",
       "   3.3,\n",
       "   -6.0,\n",
       "   -4.6,\n",
       "   -5.8,\n",
       "   -5.7,\n",
       "   -8.5,\n",
       "   -0.1,\n",
       "   0.2,\n",
       "   0.8,\n",
       "   -2.0,\n",
       "   1.0,\n",
       "   -7.0,\n",
       "   -6.0,\n",
       "   -6.1,\n",
       "   -2.8,\n",
       "   -0.4,\n",
       "   -3.1,\n",
       "   -4.1,\n",
       "   -4.0,\n",
       "   -4.0,\n",
       "   0.7,\n",
       "   1.2,\n",
       "   1.5,\n",
       "   -0.4,\n",
       "   -2.9,\n",
       "   -1.0],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 54.31,\n",
       "  'pH': 6.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1733941',\n",
       "  'KEY_WORDS': 'T4 lysozyme; proline; alpha-helix; amino acid substitution;',\n",
       "  'REFERENCE': 'J BIOL CHEM 267, 2393-2399 (1992) PMID: 1733941'},\n",
       " 'P0A877-9.0': {'MUTATION': ['C81S',\n",
       "   'C81G',\n",
       "   'C81A',\n",
       "   'C81V',\n",
       "   'C118S',\n",
       "   'C118A',\n",
       "   'C118V',\n",
       "   'C154S',\n",
       "   'C154A',\n",
       "   'C154V',\n",
       "   'P57A',\n",
       "   'P62A',\n",
       "   'P132A',\n",
       "   'P132G',\n",
       "   'P96A',\n",
       "   'P207A',\n",
       "   'K109N'],\n",
       "  '∆Tm': [-4.4,\n",
       "   -4.8,\n",
       "   -1.7,\n",
       "   -4.4,\n",
       "   -8.5,\n",
       "   -4.3,\n",
       "   -4.2,\n",
       "   -8.2,\n",
       "   -2.9,\n",
       "   -3.3,\n",
       "   -0.1,\n",
       "   -1.4,\n",
       "   -2.6,\n",
       "   -2.3,\n",
       "   -6.3,\n",
       "   -6.7,\n",
       "   -1.5],\n",
       "  'UniProt_ID': 'P0A877',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 9.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8795042',\n",
       "  'KEY_WORDS': 'tryptophan synthase alpha subunit; conformational stability ;',\n",
       "  'REFERENCE': 'PROTEIN ENG 9, 425-431 (1996) PMID: 8795042'},\n",
       " 'P00648-4.4': {'MUTATION': ['S138A',\n",
       "   'I135V',\n",
       "   'I143V',\n",
       "   'I98V',\n",
       "   'T63R',\n",
       "   'T73G',\n",
       "   'S139A',\n",
       "   'R130Q',\n",
       "   'R116M',\n",
       "   'D101N',\n",
       "   'D69M',\n",
       "   'D59G',\n",
       "   'D55A'],\n",
       "  '∆Tm': [-4.81,\n",
       "   -3.37,\n",
       "   -2.29,\n",
       "   -4.53,\n",
       "   1.33,\n",
       "   -4.06,\n",
       "   -7.13,\n",
       "   -3.29,\n",
       "   -5.77,\n",
       "   -7.06,\n",
       "   -1.57,\n",
       "   -1.95,\n",
       "   -1.91],\n",
       "  'UniProt_ID': 'P00648',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 1.71,\n",
       "  'pH': 4.4,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>95%)',\n",
       "  'PubMed_ID': '7831279',\n",
       "  'KEY_WORDS': 'calorimetry; protein folding; urea',\n",
       "  'REFERENCE': 'PROTEIN ENG 7, 1089-1095 (1994) PMID: 7831279'},\n",
       " 'P00720-5.7': {'MUTATION': ['M102L',\n",
       "   'V111F',\n",
       "   'V111I',\n",
       "   'L99A',\n",
       "   'L99V',\n",
       "   'L99I',\n",
       "   'L99M',\n",
       "   'L99F',\n",
       "   'F153A',\n",
       "   'F153V',\n",
       "   'F153I',\n",
       "   'F153L',\n",
       "   'F153M',\n",
       "   'N116D',\n",
       "   'R119M'],\n",
       "  '∆Tm': [-2.31,\n",
       "   -4.63,\n",
       "   -2.6,\n",
       "   -11.4,\n",
       "   -5.2,\n",
       "   -3.7,\n",
       "   -1.5,\n",
       "   -0.7,\n",
       "   -9.3,\n",
       "   -4.5,\n",
       "   -0.5,\n",
       "   0.8,\n",
       "   -1.6,\n",
       "   1.6,\n",
       "   0.3],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 1.5,\n",
       "  'pH': 5.7,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '1569571',\n",
       "  'KEY_WORDS': 'hydrophobic core packing; protein design; energy minimization;',\n",
       "  'REFERENCE': 'J MOL BIOL 224, 1143-1159 (1992) PMID: 1569571'},\n",
       " 'P41016-7.0': {'MUTATION': ['Q2L',\n",
       "   'R3E',\n",
       "   'R3L',\n",
       "   'R3K',\n",
       "   'R3A',\n",
       "   'N11S',\n",
       "   'Y15F',\n",
       "   'E21A',\n",
       "   'G23Q',\n",
       "   'S24D',\n",
       "   'T31S',\n",
       "   'E46A',\n",
       "   'Q53E',\n",
       "   'V64T',\n",
       "   'L66E',\n",
       "   'L66A',\n",
       "   'R56E',\n",
       "   'H29E',\n",
       "   'E46K',\n",
       "   'E21K',\n",
       "   'N55K',\n",
       "   'E36K',\n",
       "   'E50K',\n",
       "   'E12K',\n",
       "   'L66L'],\n",
       "  '∆Tm': [4.0,\n",
       "   -17.8,\n",
       "   -6.0,\n",
       "   -0.8,\n",
       "   -12.0,\n",
       "   2.3,\n",
       "   -0.2,\n",
       "   -1.9,\n",
       "   -2.2,\n",
       "   0.9,\n",
       "   0.9,\n",
       "   -1.5,\n",
       "   -0.8,\n",
       "   -1.8,\n",
       "   -8.0,\n",
       "   0.0,\n",
       "   3.1,\n",
       "   -3.4,\n",
       "   -2.9,\n",
       "   -1.5,\n",
       "   0.8,\n",
       "   -0.9,\n",
       "   -3.4,\n",
       "   -2.5,\n",
       "   0.0],\n",
       "  'UniProt_ID': 'P41016',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 41.99,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '11800561',\n",
       "  'KEY_WORDS': 'cold shock protein; protein stability; coulombic interactions; thermostable protein',\n",
       "  'REFERENCE': 'J MOL BIOL 313 343-357 (2001) PMID: 11800561'},\n",
       " 'P32081-7.0': {'MUTATION': ['E3R',\n",
       "   'E3L',\n",
       "   'A46E',\n",
       "   'E66L',\n",
       "   'M1R',\n",
       "   'E3K',\n",
       "   'K65I',\n",
       "   'E66K',\n",
       "   'A46K',\n",
       "   'S48R',\n",
       "   'F27A',\n",
       "   'F17A',\n",
       "   'F15A',\n",
       "   'F38A',\n",
       "   'L2R',\n",
       "   'E3V',\n",
       "   'E43S',\n",
       "   'A46L',\n",
       "   'S48K',\n",
       "   'T64R',\n",
       "   'E66V'],\n",
       "  '∆Tm': [16.0,\n",
       "   9.1,\n",
       "   -5.0,\n",
       "   12.8,\n",
       "   10.4,\n",
       "   16.6,\n",
       "   9.6,\n",
       "   12.9,\n",
       "   8.4,\n",
       "   8.9,\n",
       "   -6.0,\n",
       "   -11.3,\n",
       "   -15.8,\n",
       "   2.2,\n",
       "   -3.1,\n",
       "   10.2,\n",
       "   0.9,\n",
       "   4.1,\n",
       "   7.7,\n",
       "   6.1,\n",
       "   10.6],\n",
       "  'UniProt_ID': 'P32081',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 42.93,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '11800561',\n",
       "  'KEY_WORDS': 'cold shock protein; protein stability; coulombic interactions; thermostable protein',\n",
       "  'REFERENCE': 'J MOL BIOL 313 343-357 (2001) PMID: 11800561'},\n",
       " 'P06654-5.5': {'MUTATION': ['D248G',\n",
       "   'A252P',\n",
       "   'T228P',\n",
       "   'G235P',\n",
       "   'K236P',\n",
       "   'V247P',\n",
       "   'A249P',\n",
       "   'A250P',\n",
       "   'T251P',\n",
       "   'V255P',\n",
       "   'D262P',\n",
       "   'A274P'],\n",
       "  '∆Tm': [-14.0,\n",
       "   -32.0,\n",
       "   -6.6,\n",
       "   -17.0,\n",
       "   -8.4,\n",
       "   6.2,\n",
       "   -1.4,\n",
       "   -4.6,\n",
       "   -20.9,\n",
       "   -22.6,\n",
       "   -21.0,\n",
       "   -6.8],\n",
       "  'UniProt_ID': 'P06654',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 50.66,\n",
       "  'pH': 5.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '7716165',\n",
       "  'KEY_WORDS': 'phage display; protein stability; genetic selection',\n",
       "  'REFERENCE': 'PROTEINS 21 11-21 (1995) PMID: 7716165'},\n",
       " 'P0A7Y4-3.2': {'MUTATION': ['A52I',\n",
       "   'A52V',\n",
       "   'A52L',\n",
       "   'A52C',\n",
       "   'A52M',\n",
       "   'A52F',\n",
       "   'A52T',\n",
       "   'A52Q',\n",
       "   'A52E',\n",
       "   'A52P',\n",
       "   'A52S',\n",
       "   'A52N',\n",
       "   'A52D',\n",
       "   'A52Y',\n",
       "   'A52G',\n",
       "   'A52H',\n",
       "   'A52K'],\n",
       "  '∆Tm': [6.2,\n",
       "   5.5,\n",
       "   4.3,\n",
       "   2.5,\n",
       "   1.6,\n",
       "   -1.5,\n",
       "   -2.7,\n",
       "   -3.9,\n",
       "   -5.0,\n",
       "   -5.4,\n",
       "   -5.8,\n",
       "   -5.9,\n",
       "   -6.1,\n",
       "   -7.6,\n",
       "   -8.9,\n",
       "   -11.8,\n",
       "   -19.5],\n",
       "  'UniProt_ID': 'P0A7Y4',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 3.2,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '9228039',\n",
       "  'KEY_WORDS': 'Escherichia coli ribonuclease HI; thermal denaturation; hydrophobicity;',\n",
       "  'REFERENCE': 'J BIOL CHEM 272, 18686-18693 (1997) PMID: 9228039'},\n",
       " 'P06241-8.0': {'MUTATION': ['F87S',\n",
       "   'F87A',\n",
       "   'F87V',\n",
       "   'F87L',\n",
       "   'A89S',\n",
       "   'A89G',\n",
       "   'L101V',\n",
       "   'L101I',\n",
       "   'L101F',\n",
       "   'F103S',\n",
       "   'F103A',\n",
       "   'F103V',\n",
       "   'F103L',\n",
       "   'F109V',\n",
       "   'F109I',\n",
       "   'F109L',\n",
       "   'I111S',\n",
       "   'I111A',\n",
       "   'I111V',\n",
       "   'I111L',\n",
       "   'I111F',\n",
       "   'A122S',\n",
       "   'A122G',\n",
       "   'A122V',\n",
       "   'A122L',\n",
       "   'A122F',\n",
       "   'I133V',\n",
       "   'I133L',\n",
       "   'I133F',\n",
       "   'V138A',\n",
       "   'V138I',\n",
       "   'V138L',\n",
       "   'V138F',\n",
       "   'A89V',\n",
       "   'A89L',\n",
       "   'A89F',\n",
       "   'L101S',\n",
       "   'L101A',\n",
       "   'W120S',\n",
       "   'W120A',\n",
       "   'W120V',\n",
       "   'W120L',\n",
       "   'W120F',\n",
       "   'I133A',\n",
       "   'V138S',\n",
       "   'N136A',\n",
       "   'N136I',\n",
       "   'N136F',\n",
       "   'N136K',\n",
       "   'N136D',\n",
       "   'N136S',\n",
       "   'N136L',\n",
       "   'N136V',\n",
       "   'N136P',\n",
       "   'N136Y',\n",
       "   'N136H',\n",
       "   'N136E',\n",
       "   'N136T',\n",
       "   'N136R',\n",
       "   'N136Q',\n",
       "   'N136M',\n",
       "   'N136W',\n",
       "   'N136G',\n",
       "   'L86A',\n",
       "   'R123I',\n",
       "   'T130I',\n",
       "   'E107D',\n",
       "   'E107T',\n",
       "   'E107Q',\n",
       "   'E107H',\n",
       "   'E107K',\n",
       "   'E107A',\n",
       "   'E107S',\n",
       "   'E107F',\n",
       "   'E107V',\n",
       "   'E107P',\n",
       "   'E107Y',\n",
       "   'E107I',\n",
       "   'E107L',\n",
       "   'E107R',\n",
       "   'S124K',\n",
       "   'S124R',\n",
       "   'S124N',\n",
       "   'S124L',\n",
       "   'S124H',\n",
       "   'S124A',\n",
       "   'S124I',\n",
       "   'S124V',\n",
       "   'S124T',\n",
       "   'S124F',\n",
       "   'S124Y',\n",
       "   'S124D',\n",
       "   'S124G'],\n",
       "  '∆Tm': [-27.2,\n",
       "   -8.9,\n",
       "   -1.3,\n",
       "   -14.6,\n",
       "   -12.6,\n",
       "   -18.6,\n",
       "   -10.7,\n",
       "   -1.3,\n",
       "   -5.5,\n",
       "   -40.9,\n",
       "   -22.3,\n",
       "   -8.4,\n",
       "   -10.9,\n",
       "   -16.7,\n",
       "   -11.6,\n",
       "   -2.6,\n",
       "   -33.5,\n",
       "   -27.1,\n",
       "   -2.2,\n",
       "   -7.2,\n",
       "   -22.6,\n",
       "   -26.9,\n",
       "   -12.0,\n",
       "   -0.6,\n",
       "   -31.7,\n",
       "   -21.9,\n",
       "   -2.2,\n",
       "   -4.9,\n",
       "   -34.8,\n",
       "   -17.1,\n",
       "   0.9,\n",
       "   -0.2,\n",
       "   -7.1,\n",
       "   -41.5,\n",
       "   -29.3,\n",
       "   -40.7,\n",
       "   -43.0,\n",
       "   -43.8,\n",
       "   -25.6,\n",
       "   -21.6,\n",
       "   -8.3,\n",
       "   -3.2,\n",
       "   -4.4,\n",
       "   -35.9,\n",
       "   1.5,\n",
       "   2.36,\n",
       "   -1.16,\n",
       "   -3.2,\n",
       "   -0.2,\n",
       "   -2.0,\n",
       "   -0.1,\n",
       "   -0.9,\n",
       "   -5.9,\n",
       "   -13.0,\n",
       "   -2.44,\n",
       "   -2.7,\n",
       "   -4.7,\n",
       "   0.63,\n",
       "   1.79,\n",
       "   0.46,\n",
       "   0.27,\n",
       "   4.96,\n",
       "   -0.64,\n",
       "   -10.5,\n",
       "   3.2,\n",
       "   1.9,\n",
       "   2.1,\n",
       "   -5.7,\n",
       "   -6.0,\n",
       "   -6.5,\n",
       "   -6.6,\n",
       "   -7.6,\n",
       "   -12.3,\n",
       "   -13.5,\n",
       "   -14.3,\n",
       "   -15.9,\n",
       "   -18.9,\n",
       "   -24.9,\n",
       "   -27.1,\n",
       "   -34.5,\n",
       "   5.4,\n",
       "   2.5,\n",
       "   -2.6,\n",
       "   -4.5,\n",
       "   -5.3,\n",
       "   -6.2,\n",
       "   -6.8,\n",
       "   -9.8,\n",
       "   -14.5,\n",
       "   -18.7,\n",
       "   -18.7,\n",
       "   -20.7,\n",
       "   -21.5],\n",
       "  'UniProt_ID': 'P06241',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 8.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '11786916',\n",
       "  'KEY_WORDS': 'hydrophobic core packing; core folding nucleus; folding rate;',\n",
       "  'REFERENCE': 'NAT STRUCT BIOL 9 126-130 (2002) PMID: 11786916'},\n",
       " 'P26514-6.0': {'MUTATION': ['W126A',\n",
       "   'W126F',\n",
       "   'W126H',\n",
       "   'Y213A',\n",
       "   'Y213F',\n",
       "   'Y213S',\n",
       "   'W307A',\n",
       "   'W307F',\n",
       "   'W307H',\n",
       "   'W315A',\n",
       "   'W315F',\n",
       "   'W315H'],\n",
       "  '∆Tm': [2.0, -4.0, -1.0, -5.0, -0.9, 1.6, -4.7, -3.3, -7.3, 4.1, 0.8, 0.2],\n",
       "  'UniProt_ID': 'P26514',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 23.91,\n",
       "  'pH': 6.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '10235626',\n",
       "  'KEY_WORDS': 'family 10 hydrolase; glycosyl hydrolase; site-directed mutagenesis;',\n",
       "  'REFERENCE': 'PROTEIN ENG 12 251-257 (1999) PMID: 10235626'},\n",
       " 'P0A7Y4-9.0': {'MUTATION': ['D10N',\n",
       "   'D10A',\n",
       "   'D10E',\n",
       "   'D10S',\n",
       "   'D10H',\n",
       "   'E48Q',\n",
       "   'E48A',\n",
       "   'E48D',\n",
       "   'D70N',\n",
       "   'D70A',\n",
       "   'D70E',\n",
       "   'D134N',\n",
       "   'D134A'],\n",
       "  '∆Tm': [6.8, 13.7, 3.4, 9.2, 8.2, 1.0, -1.0, -0.8, 5.5, 3.8, 0.4, 6.4, 6.8],\n",
       "  'UniProt_ID': 'P0A7Y4',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 10.41,\n",
       "  'pH': 9.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8955106',\n",
       "  'KEY_WORDS': 'Escherichia coli ribonuclease HI; thermal stability; catalytic role;',\n",
       "  'REFERENCE': 'J BIOL CHEM 271, 32729-32736 (1996) PMID: 8955106'},\n",
       " 'P03051-7.0': {'MUTATION': ['D30G',\n",
       "   'D30Q',\n",
       "   'D30E',\n",
       "   'D30S',\n",
       "   'D30K',\n",
       "   'D30H',\n",
       "   'D30C',\n",
       "   'D30R',\n",
       "   'D30N',\n",
       "   'D30M',\n",
       "   'D30A',\n",
       "   'D30Y',\n",
       "   'D30L',\n",
       "   'D30F',\n",
       "   'D30T',\n",
       "   'D30V',\n",
       "   'D30W',\n",
       "   'D30I',\n",
       "   'D30P'],\n",
       "  '∆Tm': [11.6,\n",
       "   10.4,\n",
       "   6.2,\n",
       "   6.0,\n",
       "   5.9,\n",
       "   5.8,\n",
       "   5.3,\n",
       "   5.3,\n",
       "   5.1,\n",
       "   4.0,\n",
       "   2.5,\n",
       "   1.7,\n",
       "   -0.3,\n",
       "   -0.4,\n",
       "   -2.1,\n",
       "   -2.1,\n",
       "   -2.8,\n",
       "   -5.3,\n",
       "   -9.8],\n",
       "  'UniProt_ID': 'P03051',\n",
       "  'SEC_STR': 'Turn',\n",
       "  'ASA': 97.15,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '8548455',\n",
       "  'KEY_WORDS': 'protein stability; four-helix bundle; Rop; turns;',\n",
       "  'REFERENCE': 'NAT STRUCT BIOL 3, 54-58 (1996) PMID: 8548455'},\n",
       " 'P03034-8.0': {'MUTATION': ['G49S',\n",
       "   'G49N',\n",
       "   'Y89C',\n",
       "   'G47A',\n",
       "   'G49A',\n",
       "   'K5Q',\n",
       "   'Q34Y',\n",
       "   'Q45Y',\n",
       "   'A50V',\n",
       "   'Y23H',\n",
       "   'A67T',\n",
       "   'I85S'],\n",
       "  '∆Tm': [4.0, 4.1, 8.8, 3.1, 4.7, 2.0, 5.9, -0.1, -13.0, -22.7, -22.5, -14.3],\n",
       "  'UniProt_ID': 'P03034',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 75.86,\n",
       "  'pH': 8.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'no',\n",
       "  'PubMed_ID': '3449850',\n",
       "  'KEY_WORDS': 'alpha helix; lambda repressor; stabilizing effect;',\n",
       "  'REFERENCE': 'PROTEINS 1, 43-46 (1986) PMID: 3449850'},\n",
       " 'P00698-6.4': {'MUTATION': ['F21Y',\n",
       "   'H33L',\n",
       "   'N37K',\n",
       "   'R39Q',\n",
       "   'F52Y',\n",
       "   'R86K',\n",
       "   'R91K',\n",
       "   'N95H',\n",
       "   'D119G',\n",
       "   'G120V',\n",
       "   'G120R',\n",
       "   'R132H',\n",
       "   'Q139H',\n",
       "   'A49V',\n",
       "   'A49I',\n",
       "   'A49L',\n",
       "   'D119S',\n",
       "   'D119A',\n",
       "   'D119F',\n",
       "   'D119R',\n",
       "   'D119K',\n",
       "   'D119N',\n",
       "   'D119E',\n",
       "   'D119Q',\n",
       "   'S109T',\n",
       "   'S109V',\n",
       "   'S109A',\n",
       "   'T58S',\n",
       "   'I73L',\n",
       "   'I73V',\n",
       "   'T58I',\n",
       "   'I73M',\n",
       "   'S109D',\n",
       "   'I73F',\n",
       "   'S109Y',\n",
       "   'I73A',\n",
       "   'I73T'],\n",
       "  '∆Tm': [-1.2,\n",
       "   2.0,\n",
       "   -2.8,\n",
       "   -0.4,\n",
       "   0.5,\n",
       "   -0.1,\n",
       "   0.6,\n",
       "   -1.0,\n",
       "   1.2,\n",
       "   -0.1,\n",
       "   1.0,\n",
       "   1.8,\n",
       "   -1.2,\n",
       "   3.1,\n",
       "   3.6,\n",
       "   4.7,\n",
       "   2.3,\n",
       "   2.0,\n",
       "   1.9,\n",
       "   0.7,\n",
       "   0.5,\n",
       "   0.1,\n",
       "   0.0,\n",
       "   -0.2,\n",
       "   2.6,\n",
       "   -0.2,\n",
       "   -0.4,\n",
       "   -0.7,\n",
       "   -1.2,\n",
       "   -2.4,\n",
       "   -5.8,\n",
       "   -6.0,\n",
       "   -6.1,\n",
       "   -6.5,\n",
       "   -8.1,\n",
       "   -11.6,\n",
       "   -13.1],\n",
       "  'UniProt_ID': 'P00698',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 7.97,\n",
       "  'pH': 6.4,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8535242',\n",
       "  'KEY_WORDS': 'alpha-helix propensity; avian lysozyme sequences;',\n",
       "  'REFERENCE': 'PROTEIN SCI 4, 2063-2072 (1995) PMID: 8535242'},\n",
       " 'P01006-3.2': {'MUTATION': ['M134A',\n",
       "   'M134V',\n",
       "   'M134I',\n",
       "   'M134L',\n",
       "   'M104K',\n",
       "   'M104D',\n",
       "   'M104E',\n",
       "   'M104G',\n",
       "   'M104A',\n",
       "   'M104V',\n",
       "   'M104L',\n",
       "   'M104I',\n",
       "   'D114N'],\n",
       "  '∆Tm': [-4.1,\n",
       "   -4.3,\n",
       "   -7.3,\n",
       "   2.35,\n",
       "   3.71,\n",
       "   3.46,\n",
       "   0.98,\n",
       "   -0.7,\n",
       "   1.66,\n",
       "   -1.4,\n",
       "   -0.43,\n",
       "   -2.31,\n",
       "   -2.3],\n",
       "  'UniProt_ID': 'P01006',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 3.2,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '7783215',\n",
       "  'KEY_WORDS': 'calorimetry; protein stability; hydrophobicity;',\n",
       "  'REFERENCE': 'J MOL BIOL 249, 625-635 (1995) PMID: 7783215'},\n",
       " 'P01006-7.0': {'MUTATION': ['M134G',\n",
       "   'M134A',\n",
       "   'M134V',\n",
       "   'M134I',\n",
       "   'M134L',\n",
       "   'V44A',\n",
       "   'V44G',\n",
       "   'V44F',\n",
       "   'V44I',\n",
       "   'V44L',\n",
       "   'V44M',\n",
       "   'M104K',\n",
       "   'M104D',\n",
       "   'M104E',\n",
       "   'M104G',\n",
       "   'M104A',\n",
       "   'M104V',\n",
       "   'M104L',\n",
       "   'M104I',\n",
       "   'D114C',\n",
       "   'D114N'],\n",
       "  '∆Tm': [-13.57,\n",
       "   -4.2,\n",
       "   -3.6,\n",
       "   -3.9,\n",
       "   0.3,\n",
       "   -13.2,\n",
       "   -27.7,\n",
       "   -13.5,\n",
       "   -2.4,\n",
       "   -4.5,\n",
       "   -12.6,\n",
       "   0.65,\n",
       "   3.08,\n",
       "   1.99,\n",
       "   -0.19,\n",
       "   1.16,\n",
       "   -1.17,\n",
       "   -0.46,\n",
       "   -1.78,\n",
       "   13.3,\n",
       "   1.07],\n",
       "  'UniProt_ID': 'P01006',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '7783215',\n",
       "  'KEY_WORDS': 'calorimetry; protein stability; hydrophobicity;',\n",
       "  'REFERENCE': 'J MOL BIOL 249, 625-635 (1995) PMID: 7783215'},\n",
       " 'P01006-9.5': {'MUTATION': ['M134G',\n",
       "   'M134A',\n",
       "   'M134V',\n",
       "   'M134I',\n",
       "   'M134L',\n",
       "   'V44A',\n",
       "   'V44G',\n",
       "   'V44F',\n",
       "   'V44I',\n",
       "   'V44L',\n",
       "   'V44M',\n",
       "   'M104K',\n",
       "   'M104D',\n",
       "   'M104E',\n",
       "   'M104G',\n",
       "   'M104A',\n",
       "   'M104V',\n",
       "   'M104L',\n",
       "   'M104I',\n",
       "   'D114C',\n",
       "   'D114N'],\n",
       "  '∆Tm': [-14.4,\n",
       "   -5.18,\n",
       "   -4.33,\n",
       "   -4.8,\n",
       "   -0.48,\n",
       "   -13.7,\n",
       "   -25.4,\n",
       "   -12.4,\n",
       "   -1.83,\n",
       "   -5.3,\n",
       "   -11.7,\n",
       "   0.17,\n",
       "   2.59,\n",
       "   1.06,\n",
       "   -0.87,\n",
       "   0.18,\n",
       "   -1.75,\n",
       "   -1.05,\n",
       "   -2.42,\n",
       "   14.27,\n",
       "   1.88],\n",
       "  'UniProt_ID': 'P01006',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 9.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '7783215',\n",
       "  'KEY_WORDS': 'calorimetry; protein stability; hydrophobicity;',\n",
       "  'REFERENCE': 'J MOL BIOL 249, 625-635 (1995) PMID: 7783215'},\n",
       " 'P00648-6.3': {'MUTATION': ['I143V',\n",
       "   'I135V',\n",
       "   'I143A',\n",
       "   'I135A',\n",
       "   'L61A',\n",
       "   'D59G',\n",
       "   'D59A',\n",
       "   'Y60G',\n",
       "   'Q62G',\n",
       "   'Q62A',\n",
       "   'Y64G',\n",
       "   'I102G',\n",
       "   'R119G',\n",
       "   'E120G',\n",
       "   'I135G',\n",
       "   'L136G',\n",
       "   'L142G',\n",
       "   'I143G',\n",
       "   'Y144G',\n",
       "   'T147G',\n",
       "   'G100A',\n",
       "   'G99A',\n",
       "   'G100V',\n",
       "   'G99V'],\n",
       "  '∆Tm': [-2.4,\n",
       "   -2.9,\n",
       "   -9.6,\n",
       "   -11.2,\n",
       "   -11.9,\n",
       "   -2.3,\n",
       "   -1.0,\n",
       "   -15.6,\n",
       "   -2.0,\n",
       "   -0.9,\n",
       "   -10.3,\n",
       "   -5.3,\n",
       "   -5.7,\n",
       "   -12.7,\n",
       "   -20.8,\n",
       "   -8.7,\n",
       "   -9.1,\n",
       "   -15.7,\n",
       "   -17.9,\n",
       "   -4.3,\n",
       "   -8.6,\n",
       "   -13.3,\n",
       "   -19.6,\n",
       "   -21.1],\n",
       "  'UniProt_ID': 'P00648',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 6.3,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>85%)',\n",
       "  'PubMed_ID': '2669964',\n",
       "  'KEY_WORDS': 'side-chain packing; hydrophobic core; energetics; barnase;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 28, 4914-4922 (1989) PMID: 2669964'},\n",
       " 'P05798-7.0': {'MUTATION': ['N39D',\n",
       "   'N39S',\n",
       "   'N39A',\n",
       "   'T18V',\n",
       "   'T56V',\n",
       "   'T67V',\n",
       "   'T82V',\n",
       "   'T5V',\n",
       "   'T16V',\n",
       "   'T59V',\n",
       "   'T72V',\n",
       "   'V2T',\n",
       "   'V36T',\n",
       "   'V43T',\n",
       "   'V57T',\n",
       "   'Q38A',\n",
       "   'E41K',\n",
       "   'E54Q',\n",
       "   'R65A',\n",
       "   'E74K',\n",
       "   'H85Q',\n",
       "   'D79F',\n",
       "   'D79Y',\n",
       "   'D79A',\n",
       "   'D79I',\n",
       "   'D79R',\n",
       "   'D79L',\n",
       "   'D79K',\n",
       "   'D79W',\n",
       "   'D79H',\n",
       "   'D79N',\n",
       "   'D79E',\n",
       "   'Q94K',\n",
       "   'D33A',\n",
       "   'N20A',\n",
       "   'S3A',\n",
       "   'S9A',\n",
       "   'S24A',\n",
       "   'S31A',\n",
       "   'S42A',\n",
       "   'S48A',\n",
       "   'S90A',\n",
       "   'T95A',\n",
       "   'L8A',\n",
       "   'L11A',\n",
       "   'L19A',\n",
       "   'L21A',\n",
       "   'I22V',\n",
       "   'I58V',\n",
       "   'I70V',\n",
       "   'I71V',\n",
       "   'L91A',\n",
       "   'I92V'],\n",
       "  '∆Tm': [-5.2,\n",
       "   -8.0,\n",
       "   -7.6,\n",
       "   -4.7,\n",
       "   -6.3,\n",
       "   -0.1,\n",
       "   -5.7,\n",
       "   0.0,\n",
       "   1.0,\n",
       "   -5.6,\n",
       "   -0.8,\n",
       "   -3.2,\n",
       "   -4.6,\n",
       "   -1.6,\n",
       "   -15.0,\n",
       "   3.5,\n",
       "   -2.5,\n",
       "   -5.9,\n",
       "   -3.4,\n",
       "   3.1,\n",
       "   0.1,\n",
       "   10.0,\n",
       "   9.6,\n",
       "   9.2,\n",
       "   9.6,\n",
       "   9.0,\n",
       "   8.7,\n",
       "   7.6,\n",
       "   7.6,\n",
       "   5.6,\n",
       "   5.5,\n",
       "   -0.8,\n",
       "   0.8,\n",
       "   -16.0,\n",
       "   -0.1,\n",
       "   -1.1,\n",
       "   -4.5,\n",
       "   -0.7,\n",
       "   1.5,\n",
       "   0.4,\n",
       "   1.7,\n",
       "   0.7,\n",
       "   -2.1,\n",
       "   -5.0,\n",
       "   -11.9,\n",
       "   -13.8,\n",
       "   -1.6,\n",
       "   -5.7,\n",
       "   0.11,\n",
       "   -3.4,\n",
       "   -0.3,\n",
       "   -3.2,\n",
       "   -3.9],\n",
       "  'UniProt_ID': 'P05798',\n",
       "  'SEC_STR': 'Turn',\n",
       "  'ASA': 9.56,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'Yes',\n",
       "  'PubMed_ID': '9819211',\n",
       "  'KEY_WORDS': 'RNase Sa, and T1, Conformational stability, inter and intramolecular hydrogen bonds, conserved asparagine.',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 37, 16192-16200 (1998) PMID: 9819211'},\n",
       " 'P00257-8.5': {'MUTATION': ['F69W',\n",
       "   'F101W',\n",
       "   'F117W',\n",
       "   'F122W',\n",
       "   'Y140W',\n",
       "   'H114Q',\n",
       "   'H114T',\n",
       "   'H114R',\n",
       "   'T112S',\n",
       "   'T112A',\n",
       "   'Y140S',\n",
       "   'Y140L',\n",
       "   'Y140F',\n",
       "   'C153S',\n",
       "   'D134E'],\n",
       "  '∆Tm': [-5.3,\n",
       "   -6.5,\n",
       "   -14.6,\n",
       "   -3.2,\n",
       "   -0.7,\n",
       "   -4.94,\n",
       "   -2.64,\n",
       "   -2.11,\n",
       "   0.21,\n",
       "   -4.29,\n",
       "   -0.79,\n",
       "   -0.69,\n",
       "   0.11,\n",
       "   4.36,\n",
       "   2.01],\n",
       "  'UniProt_ID': 'P00257',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 8.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'YES',\n",
       "  'PubMed_ID': '12206673',\n",
       "  'KEY_WORDS': 'protein structure; aromatic amino acids; biological function; unfolding mechanism',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 41, 11008-11016 (2002) PMID: 12206673'},\n",
       " 'P00171-7.0': {'MUTATION': ['I41L',\n",
       "   'L87S',\n",
       "   'S23C',\n",
       "   'F40Y',\n",
       "   'F40L',\n",
       "   'F40H',\n",
       "   'V66E',\n",
       "   'V66Y',\n",
       "   'V66H',\n",
       "   'V66K'],\n",
       "  '∆Tm': [-0.8, -10.2, 0.3, 3.4, -7.1, -11.3, -4.0, -4.7, -5.9, -8.7],\n",
       "  'UniProt_ID': 'P00171',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 2.16,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'YES',\n",
       "  'PubMed_ID': '12269800',\n",
       "  'KEY_WORDS': 'hemin-binding; microsomal; hydrophobic networks; protonation',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 41, 11566-11581 (2002) PMID: 12269800'},\n",
       " 'P39940-3.0': {'MUTATION': ['E34L',\n",
       "   'E34T',\n",
       "   'E34I',\n",
       "   'E34W',\n",
       "   'E34C',\n",
       "   'E34F',\n",
       "   'E34V',\n",
       "   'E34Y',\n",
       "   'E34A',\n",
       "   'E34S',\n",
       "   'E34D',\n",
       "   'E34N',\n",
       "   'E34Q',\n",
       "   'E34K',\n",
       "   'E34G',\n",
       "   'E34H',\n",
       "   'E34P',\n",
       "   'I334L',\n",
       "   'I340L',\n",
       "   'I340V',\n",
       "   'I334V',\n",
       "   'I334F',\n",
       "   'I340F'],\n",
       "  '∆Tm': [8.8,\n",
       "   10.0,\n",
       "   10.5,\n",
       "   8.5,\n",
       "   6.0,\n",
       "   4.7,\n",
       "   5.7,\n",
       "   2.7,\n",
       "   0.3,\n",
       "   0.7,\n",
       "   -1.7,\n",
       "   -7.1,\n",
       "   -13.0,\n",
       "   -18.6,\n",
       "   -14.2,\n",
       "   -43.6,\n",
       "   -30.3,\n",
       "   -12.5,\n",
       "   -8.7,\n",
       "   -3.2,\n",
       "   -9.9,\n",
       "   -26.0,\n",
       "   -3.8],\n",
       "  'UniProt_ID': 'P39940',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 51.52,\n",
       "  'pH': 3.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes(>90%)',\n",
       "  'PubMed_ID': '12215419',\n",
       "  'KEY_WORDS': 'helix termination; C-capping motifs; statistical analysis; hydrophobic interactions; differential scanning calorimetry',\n",
       "  'REFERENCE': 'J MOL BIOL 322, 123-135 (2002) PMID: 12215419'},\n",
       " 'P42771-8.5': {'MUTATION': ['T18R',\n",
       "   'L37S',\n",
       "   'A73E',\n",
       "   'L121R',\n",
       "   'L65K',\n",
       "   'L78G',\n",
       "   'V95K',\n",
       "   'W15D',\n",
       "   'R124D',\n",
       "   'R128E'],\n",
       "  '∆Tm': [-1.0, 4.3, 0.2, 4.4, 1.2, 0.9, 1.0, 2.2, -0.4, 1.6],\n",
       "  'UniProt_ID': 'P42771',\n",
       "  'SEC_STR': 'Turn',\n",
       "  'ASA': 36.05,\n",
       "  'pH': 8.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '12614625',\n",
       "  'KEY_WORDS': 'p16INK4a; cyclin-dependent kinase inhibitor; cancer-related mutations; protein design; stability',\n",
       "  'REFERENCE': 'J MOL BIOL 327, 285-297 (2003) PMID: 12614625'},\n",
       " 'O00095-8.0': {'MUTATION': ['A51V',\n",
       "   'W23Y',\n",
       "   'A51S',\n",
       "   'S55N',\n",
       "   'G57A',\n",
       "   'S79V',\n",
       "   'A82N',\n",
       "   'S93G',\n",
       "   'N107D',\n",
       "   'S159T',\n",
       "   'T179S',\n",
       "   'N183S',\n",
       "   'A204G',\n",
       "   'G186C',\n",
       "   'P217C',\n",
       "   'V226C'],\n",
       "  '∆Tm': [7.7,\n",
       "   -1.0,\n",
       "   -4.0,\n",
       "   0.5,\n",
       "   2.5,\n",
       "   -0.8,\n",
       "   0.1,\n",
       "   0.1,\n",
       "   0.5,\n",
       "   0.5,\n",
       "   0.3,\n",
       "   0.2,\n",
       "   0.5,\n",
       "   2.1,\n",
       "   3.9,\n",
       "   0.1],\n",
       "  'UniProt_ID': 'O00095',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 4.54,\n",
       "  'pH': 8.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'No',\n",
       "  'PubMed_ID': '12649442',\n",
       "  'KEY_WORDS': 'Thermal stability; cellulase; endoglucanase; homolog; protein crystal structure',\n",
       "  'REFERENCE': 'PROTEIN SCI 12, 848-860 (2003) PMID: 12649442'},\n",
       " 'P00720-5.35': {'MUTATION': ['I78V',\n",
       "   'L118I',\n",
       "   'V87I',\n",
       "   'I100V',\n",
       "   'M102L',\n",
       "   'V103I',\n",
       "   'M106I',\n",
       "   'V111A',\n",
       "   'M120Y',\n",
       "   'L133F',\n",
       "   'V149I',\n",
       "   'R96K',\n",
       "   'R96Q',\n",
       "   'R96A',\n",
       "   'R96V',\n",
       "   'R96S',\n",
       "   'R96E',\n",
       "   'R96G',\n",
       "   'R96M',\n",
       "   'R96T',\n",
       "   'R96C',\n",
       "   'R96I',\n",
       "   'R96N',\n",
       "   'R96H',\n",
       "   'R96L',\n",
       "   'R96D',\n",
       "   'R96F',\n",
       "   'R96W',\n",
       "   'R96Y',\n",
       "   'R96P',\n",
       "   'K85A',\n",
       "   'D89A'],\n",
       "  '∆Tm': [-2.1,\n",
       "   -3.1,\n",
       "   -0.8,\n",
       "   -1.1,\n",
       "   -2.3,\n",
       "   -1.5,\n",
       "   0.6,\n",
       "   -2.9,\n",
       "   -0.1,\n",
       "   -0.7,\n",
       "   -0.3,\n",
       "   -0.2,\n",
       "   -1.4,\n",
       "   -5.1,\n",
       "   -6.4,\n",
       "   -7.0,\n",
       "   -7.0,\n",
       "   -7.1,\n",
       "   -7.1,\n",
       "   -7.6,\n",
       "   -7.7,\n",
       "   -7.9,\n",
       "   -8.0,\n",
       "   -8.3,\n",
       "   -8.6,\n",
       "   -9.5,\n",
       "   -11.5,\n",
       "   -12.8,\n",
       "   -13.2,\n",
       "   -15.5,\n",
       "   -1.0,\n",
       "   -1.3],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 5.35,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '12963380',\n",
       "  'KEY_WORDS': 'ORBIT; single-site revertants; T4 lysozyme; repacking',\n",
       "  'REFERENCE': 'J MOL BIOL 332, 741-756 (2003) PMID: 12963380'},\n",
       " 'P0A3E0-7.0': {'MUTATION': ['E21K',\n",
       "   'E41K',\n",
       "   'E62K',\n",
       "   'D66K',\n",
       "   'E73K',\n",
       "   'D76K',\n",
       "   'D127K',\n",
       "   'D151K',\n",
       "   'L7A',\n",
       "   'I23V',\n",
       "   'V32A',\n",
       "   'D44A',\n",
       "   'I52V',\n",
       "   'I53V',\n",
       "   'S72A',\n",
       "   'A85G',\n",
       "   'N98A',\n",
       "   'Q100A',\n",
       "   'I105V',\n",
       "   'S111A',\n",
       "   'V118A',\n",
       "   'T123S',\n",
       "   'V140A',\n",
       "   'L144A',\n",
       "   'I157V',\n",
       "   'V161A',\n",
       "   'V19I',\n",
       "   'I23A',\n",
       "   'I23G',\n",
       "   'L51A',\n",
       "   'G69A',\n",
       "   'V84I',\n",
       "   'A102V',\n",
       "   'E108A',\n",
       "   'Q112G'],\n",
       "  '∆Tm': [-1.5,\n",
       "   0.6,\n",
       "   3.6,\n",
       "   -2.1,\n",
       "   1.2,\n",
       "   0.1,\n",
       "   4.6,\n",
       "   -0.1,\n",
       "   -11.8,\n",
       "   -6.3,\n",
       "   -4.7,\n",
       "   0.4,\n",
       "   -4.1,\n",
       "   -1.4,\n",
       "   -1.0,\n",
       "   -4.3,\n",
       "   -2.4,\n",
       "   5.2,\n",
       "   -4.0,\n",
       "   -1.7,\n",
       "   -8.0,\n",
       "   5.9,\n",
       "   -6.4,\n",
       "   -1.5,\n",
       "   -8.4,\n",
       "   -8.2,\n",
       "   4.9,\n",
       "   -0.5,\n",
       "   3.8,\n",
       "   0.6,\n",
       "   6.7,\n",
       "   1.0,\n",
       "   5.4,\n",
       "   2.7,\n",
       "   3.7],\n",
       "  'UniProt_ID': 'P0A3E0',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 46.94,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '3',\n",
       "  'REVERSIBILITY': 'Yes',\n",
       "  'PubMed_ID': '15504413',\n",
       "  'KEY_WORDS': 'charge/charge interaction; protein stability; protein stabilisation; protein folding; equilibrium intermediate',\n",
       "  'REFERENCE': 'J MOL BIOL 344, 223-237 (2004); doi:10.1016/j.jmb.2004.09.047 PMID: 15504413'},\n",
       " 'P05230-7.3': {'MUTATION': ['H36Y',\n",
       "   'L59F',\n",
       "   'H117Y',\n",
       "   'F123Y',\n",
       "   'V124I',\n",
       "   'K133E',\n",
       "   'K127N',\n",
       "   'K127A',\n",
       "   'K127E',\n",
       "   'K133A',\n",
       "   'K133N'],\n",
       "  '∆Tm': [3.6, 1.5, 1.9, 1.6, -0.3, 0.9, -3.5, 0.3, 0.2, 0.7, 11.8],\n",
       "  'UniProt_ID': 'P05230',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 4.4,\n",
       "  'pH': 7.3,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Yes',\n",
       "  'PubMed_ID': '15469994',\n",
       "  'KEY_WORDS': 'DNA synthesis; fibroblast growth factor; MAP kinase activation; protein engineering; stability',\n",
       "  'REFERENCE': 'PROTEIN ENG DES SEL 17, 603-611 (2004) PMID: 15469994'},\n",
       " 'P29160-7.4': {'MUTATION': ['E7A',\n",
       "   'K10A',\n",
       "   'K10M',\n",
       "   'E91A',\n",
       "   'R93A',\n",
       "   'R93M',\n",
       "   'D3A',\n",
       "   'R9A',\n",
       "   'D13A',\n",
       "   'K16A',\n",
       "   'R22A',\n",
       "   'K23A',\n",
       "   'K29A',\n",
       "   'K34A',\n",
       "   'R40A',\n",
       "   'R43A',\n",
       "   'D45A',\n",
       "   'K47A',\n",
       "   'E48A',\n",
       "   'D49A',\n",
       "   'E51A',\n",
       "   'R55A',\n",
       "   'E63A',\n",
       "   'E65A',\n",
       "   'E70A',\n",
       "   'R77A',\n",
       "   'H79A',\n",
       "   'D88A'],\n",
       "  '∆Tm': [-8.6,\n",
       "   -8.4,\n",
       "   -7.1,\n",
       "   -0.8,\n",
       "   -3.7,\n",
       "   -3.1,\n",
       "   -2.5,\n",
       "   -7.0,\n",
       "   0.4,\n",
       "   -6.1,\n",
       "   -1.5,\n",
       "   1.0,\n",
       "   -4.4,\n",
       "   -6.4,\n",
       "   -4.8,\n",
       "   0.2,\n",
       "   0.1,\n",
       "   -7.4,\n",
       "   2.0,\n",
       "   3.2,\n",
       "   2.3,\n",
       "   -5.4,\n",
       "   -5.5,\n",
       "   0.4,\n",
       "   -2.0,\n",
       "   -1.5,\n",
       "   -2.2,\n",
       "   -9.2],\n",
       "  'UniProt_ID': 'P29160',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 2.86,\n",
       "  'pH': 7.4,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'No',\n",
       "  'PubMed_ID': '15811378',\n",
       "  'KEY_WORDS': 'ion pairs; thermostability; site-directed mutagenesis; protein stability; hyperthermophiles',\n",
       "  'REFERENCE': 'J MOL BIOL 348, 419-431 (2005) PMID: 15811378'},\n",
       " 'P11961-5.5': {'MUTATION': ['V129A',\n",
       "   'V129G',\n",
       "   'I130V',\n",
       "   'I130A',\n",
       "   'I130G',\n",
       "   'A131G',\n",
       "   'V136A',\n",
       "   'Y138F',\n",
       "   'A139G',\n",
       "   'E141Q',\n",
       "   'E141A',\n",
       "   'V145A',\n",
       "   'D145N',\n",
       "   'D145A',\n",
       "   'I147V',\n",
       "   'I147A',\n",
       "   'V149A',\n",
       "   'V149G',\n",
       "   'V158A',\n",
       "   'L159V',\n",
       "   'L159A',\n",
       "   'L159G',\n",
       "   'D162N',\n",
       "   'I163V',\n",
       "   'D164N',\n",
       "   'D164A',\n",
       "   'D164G',\n",
       "   'A166G',\n",
       "   'L167V',\n",
       "   'L167A',\n",
       "   'A168G'],\n",
       "  '∆Tm': [-5.6,\n",
       "   -8.6,\n",
       "   -1.4,\n",
       "   -5.5,\n",
       "   -12.0,\n",
       "   -15.3,\n",
       "   -13.3,\n",
       "   -16.4,\n",
       "   -21.6,\n",
       "   -2.9,\n",
       "   -5.5,\n",
       "   -17.3,\n",
       "   -7.7,\n",
       "   -18.0,\n",
       "   -10.3,\n",
       "   -27.4,\n",
       "   -23.8,\n",
       "   -39.2,\n",
       "   -28.1,\n",
       "   -4.1,\n",
       "   -18.4,\n",
       "   -16.5,\n",
       "   -15.9,\n",
       "   -4.5,\n",
       "   -14.9,\n",
       "   -19.4,\n",
       "   -29.7,\n",
       "   -7.5,\n",
       "   -1.1,\n",
       "   -9.5,\n",
       "   -2.6],\n",
       "  'UniProt_ID': 'P11961',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 25.63,\n",
       "  'pH': 5.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Yes',\n",
       "  'PubMed_ID': '15769475',\n",
       "  'KEY_WORDS': 'fast folding; molecular dynamics; transition state; folding pathway; temperature-jump',\n",
       "  'REFERENCE': 'J MOL BIOL 347, 855-870 (2005) PMID: 15769475'},\n",
       " 'P02640-7.0': {'MUTATION': ['H791Y',\n",
       "   'S793A',\n",
       "   'T804V',\n",
       "   'L42A',\n",
       "   'F797A',\n",
       "   'V800A',\n",
       "   'F801A',\n",
       "   'M53A',\n",
       "   'F808A',\n",
       "   'L61A',\n",
       "   'L69A',\n",
       "   'L75A'],\n",
       "  '∆Tm': [10.0,\n",
       "   -9.4,\n",
       "   -12.8,\n",
       "   -14.0,\n",
       "   -32.7,\n",
       "   -7.4,\n",
       "   -25.6,\n",
       "   -39.7,\n",
       "   -46.1,\n",
       "   -29.1,\n",
       "   -19.1,\n",
       "   -7.2],\n",
       "  'UniProt_ID': 'P02640',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 0.55,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '16337228',\n",
       "  'KEY_WORDS': 'villin headpiece; protein folding; protein stability; hydrogen exchange; multistate folding',\n",
       "  'REFERENCE': 'J MOL BIOL 355, 1066-1077 (2006) PMID: 16337228'},\n",
       " 'P61495-7.6': {'MUTATION': ['T16V',\n",
       "   'F53L',\n",
       "   'P56E',\n",
       "   'R58L',\n",
       "   'V61I',\n",
       "   'L134N',\n",
       "   'H179K',\n",
       "   'V181T',\n",
       "   'D184H',\n",
       "   'S261N',\n",
       "   'P324T',\n",
       "   'A335E'],\n",
       "  '∆Tm': [0.7, -1.8, -0.3, -1.8, -0.3, 3.2, -1.8, 1.4, 0.1, -1.4, 0.7, 0.4],\n",
       "  'UniProt_ID': 'P61495',\n",
       "  'SEC_STR': 'Turn',\n",
       "  'ASA': 2.88,\n",
       "  'pH': 7.6,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'No',\n",
       "  'PubMed_ID': '16309701',\n",
       "  'KEY_WORDS': 'ancestral residue; protein stability; 3-isopropylmalate dehydrogenase; Thermus thermophilus; Commonote',\n",
       "  'REFERENCE': 'J MOL BIOL 355, 664-674 (2006) PMID: 16309701'},\n",
       " 'P0CG48-2.25': {'MUTATION': ['K641E',\n",
       "   'K641F',\n",
       "   'K641G',\n",
       "   'K641I',\n",
       "   'K641L',\n",
       "   'K641M',\n",
       "   'K641N',\n",
       "   'K641Q',\n",
       "   'K641S',\n",
       "   'K641T',\n",
       "   'K641V'],\n",
       "  '∆Tm': [1.6, 2.1, -13.5, 10.8, 6.9, 3.8, -4.9, 0.7, -2.4, -1.3, 6.6],\n",
       "  'UniProt_ID': 'P0CG48',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 2.25,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>90%)',\n",
       "  'PubMed_ID': '15515183',\n",
       "  'KEY_WORDS': 'protein stability; helical propensity; hydrophobicity; protein engineering; differential scanning calorimetry',\n",
       "  'REFERENCE': 'PROTEINS 58, 1-6 (2005) PMID: 15515183'},\n",
       " 'P0CG48-2.5': {'MUTATION': ['K641E',\n",
       "   'K641F',\n",
       "   'K641G',\n",
       "   'K641I',\n",
       "   'K641L',\n",
       "   'K641M',\n",
       "   'K641N',\n",
       "   'K641Q',\n",
       "   'K641S',\n",
       "   'K641T',\n",
       "   'K641V'],\n",
       "  '∆Tm': [1.4, 1.8, -12.6, 10.1, 6.6, 3.3, -5.2, 0.3, -2.8, -1.4, 6.3],\n",
       "  'UniProt_ID': 'P0CG48',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 2.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>90%)',\n",
       "  'PubMed_ID': '15515183',\n",
       "  'KEY_WORDS': 'protein stability; helical propensity; hydrophobicity; protein engineering; differential scanning calorimetry',\n",
       "  'REFERENCE': 'PROTEINS 58, 1-6 (2005) PMID: 15515183'},\n",
       " 'P0CG48-2.75': {'MUTATION': ['K641E',\n",
       "   'K641F',\n",
       "   'K641G',\n",
       "   'K641I',\n",
       "   'K641L',\n",
       "   'K641M',\n",
       "   'K641N',\n",
       "   'K641Q',\n",
       "   'K641S',\n",
       "   'K641T',\n",
       "   'K641V'],\n",
       "  '∆Tm': [1.2, 0.9, -12.2, 9.2, 5.8, 2.6, -5.4, 0.1, -3.4, -1.4, 5.9],\n",
       "  'UniProt_ID': 'P0CG48',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 2.75,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>90%)',\n",
       "  'PubMed_ID': '15515183',\n",
       "  'KEY_WORDS': 'protein stability; helical propensity; hydrophobicity; protein engineering; differential scanning calorimetry',\n",
       "  'REFERENCE': 'PROTEINS 58, 1-6 (2005) PMID: 15515183'},\n",
       " 'P0CG48-3.0': {'MUTATION': ['K641E',\n",
       "   'K641F',\n",
       "   'K641G',\n",
       "   'K641I',\n",
       "   'K641L',\n",
       "   'K641M',\n",
       "   'K641N',\n",
       "   'K641Q',\n",
       "   'K641S',\n",
       "   'K641T',\n",
       "   'K641V'],\n",
       "  '∆Tm': [0.7, 1.3, -11.1, 8.6, 5.6, 2.6, -4.7, -0.2, -2.9, -1.1, 5.3],\n",
       "  'UniProt_ID': 'P0CG48',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 3.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>90%)',\n",
       "  'PubMed_ID': '15515183',\n",
       "  'KEY_WORDS': 'protein stability; helical propensity; hydrophobicity; protein engineering; differential scanning calorimetry',\n",
       "  'REFERENCE': 'PROTEINS 58, 1-6 (2005) PMID: 15515183'},\n",
       " 'P0CG48-3.25': {'MUTATION': ['K641E',\n",
       "   'K641F',\n",
       "   'K641G',\n",
       "   'K641I',\n",
       "   'K641L',\n",
       "   'K641M',\n",
       "   'K641N',\n",
       "   'K641Q',\n",
       "   'K641S',\n",
       "   'K641T',\n",
       "   'K641V'],\n",
       "  '∆Tm': [0.1, 1.1, -9.4, 8.6, 5.6, 3.1, -3.6, 0.2, -2.2, -0.7, 5.3],\n",
       "  'UniProt_ID': 'P0CG48',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 3.25,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (>90%)',\n",
       "  'PubMed_ID': '15515183',\n",
       "  'KEY_WORDS': 'protein stability; helical propensity; hydrophobicity; protein engineering; differential scanning calorimetry',\n",
       "  'REFERENCE': 'PROTEINS 58, 1-6 (2005) PMID: 15515183'},\n",
       " 'O74035-9.0': {'MUTATION': ['D7N',\n",
       "   'E8A',\n",
       "   'E8Q',\n",
       "   'D105A',\n",
       "   'D135A',\n",
       "   'I6A',\n",
       "   'I15A',\n",
       "   'I20A',\n",
       "   'L33A',\n",
       "   'I56A',\n",
       "   'L92A',\n",
       "   'I102A',\n",
       "   'L118A',\n",
       "   'L147A'],\n",
       "  '∆Tm': [3.1,\n",
       "   8.0,\n",
       "   0.6,\n",
       "   4.2,\n",
       "   5.2,\n",
       "   -5.2,\n",
       "   -2.2,\n",
       "   -8.4,\n",
       "   -11.9,\n",
       "   -8.7,\n",
       "   -5.6,\n",
       "   -10.7,\n",
       "   -13.7,\n",
       "   -5.2],\n",
       "  'UniProt_ID': 'O74035',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 3.47,\n",
       "  'pH': 9.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Yes',\n",
       "  'PubMed_ID': '17042484',\n",
       "  'KEY_WORDS': 'conformational stability, hyperthermophiles, ribonuclease HII',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 45, 12673-12679 (2006) PMID: 17042484'},\n",
       " 'P03034-7.0': {'MUTATION': ['V37I',\n",
       "   'M41A',\n",
       "   'P79A',\n",
       "   'T9A',\n",
       "   'T9V',\n",
       "   'E11A',\n",
       "   'E11V',\n",
       "   'L13A',\n",
       "   'L13V',\n",
       "   'E24A',\n",
       "   'E24V',\n",
       "   'L58P',\n",
       "   'L58C',\n",
       "   'L58A'],\n",
       "  '∆Tm': [3.4,\n",
       "   -8.6,\n",
       "   -7.0,\n",
       "   -5.0,\n",
       "   -5.0,\n",
       "   -2.0,\n",
       "   -2.0,\n",
       "   -4.0,\n",
       "   -2.0,\n",
       "   -5.0,\n",
       "   -4.0,\n",
       "   -15.0,\n",
       "   -19.0,\n",
       "   -34.0],\n",
       "  'UniProt_ID': 'P03034',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 30.25,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes (70-100%)',\n",
       "  'PubMed_ID': '1567879',\n",
       "  'KEY_WORDS': 'lambda repressor; hydrophobic core; CD spectroscopy;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 31, 4324-4333 (1992) PMID: 1567879'},\n",
       " 'P02925-7.0': {'MUTATION': ['S34A',\n",
       "   'N38A',\n",
       "   'F40A',\n",
       "   'F41A',\n",
       "   'N89A',\n",
       "   'D114A',\n",
       "   'S128A',\n",
       "   'I157A',\n",
       "   'F189A',\n",
       "   'N215A',\n",
       "   'F239A',\n",
       "   'D240A',\n",
       "   'Q260A'],\n",
       "  '∆Tm': [-1.5,\n",
       "   0.7,\n",
       "   -5.0,\n",
       "   1.4,\n",
       "   -4.6,\n",
       "   0.7,\n",
       "   0.3,\n",
       "   -0.2,\n",
       "   -5.8,\n",
       "   -8.1,\n",
       "   -9.5,\n",
       "   -6.3,\n",
       "   0.7],\n",
       "  'UniProt_ID': 'P02925',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '17242374',\n",
       "  'KEY_WORDS': 'biosensor; periplasmic binding proteins; ribose binding protein; fluorescence',\n",
       "  'REFERENCE': 'PROTEIN SCI 16, 362-368 (2007) PMID: 17242374'},\n",
       " 'R9S082-11.0': {'MUTATION': ['I29L',\n",
       "   'I29M',\n",
       "   'I29V',\n",
       "   'I29A',\n",
       "   'I112L',\n",
       "   'I112M',\n",
       "   'I112A',\n",
       "   'I143L',\n",
       "   'I143M',\n",
       "   'I143V',\n",
       "   'I143A',\n",
       "   'L30I',\n",
       "   'L30M',\n",
       "   'L30V',\n",
       "   'L30A',\n",
       "   'L70I',\n",
       "   'L70M',\n",
       "   'L70V',\n",
       "   'L70A',\n",
       "   'L136I',\n",
       "   'L136M',\n",
       "   'L136V',\n",
       "   'L50I'],\n",
       "  '∆Tm': [-1.7,\n",
       "   -1.7,\n",
       "   0.1,\n",
       "   -4.4,\n",
       "   -2.0,\n",
       "   -3.7,\n",
       "   -6.4,\n",
       "   1.8,\n",
       "   2.6,\n",
       "   -0.3,\n",
       "   -3.5,\n",
       "   -3.6,\n",
       "   -0.3,\n",
       "   -6.0,\n",
       "   -8.9,\n",
       "   -0.1,\n",
       "   0.0,\n",
       "   -0.3,\n",
       "   -3.8,\n",
       "   -5.2,\n",
       "   -2.5,\n",
       "   -8.3,\n",
       "   -2.5],\n",
       "  'UniProt_ID': 'R9S082',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 2.16,\n",
       "  'pH': 11.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8251481',\n",
       "  'KEY_WORDS': 'hydrophobic effects; alpha-helix stability; myoglobin;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 32, 12638-12643 (1993) PMID: 8251481'},\n",
       " 'P00720-3.05': {'MUTATION': ['R96K',\n",
       "   'R96Q',\n",
       "   'R96A',\n",
       "   'R96V',\n",
       "   'R96S',\n",
       "   'R96E',\n",
       "   'R96G',\n",
       "   'R96M',\n",
       "   'R96T',\n",
       "   'R96C',\n",
       "   'R96I',\n",
       "   'R96N',\n",
       "   'R96H',\n",
       "   'R96L',\n",
       "   'R96D',\n",
       "   'R96F',\n",
       "   'R96W',\n",
       "   'R96Y',\n",
       "   'R96P',\n",
       "   'K85A',\n",
       "   'D89A'],\n",
       "  '∆Tm': [-0.7,\n",
       "   -1.2,\n",
       "   -4.3,\n",
       "   -4.8,\n",
       "   -6.6,\n",
       "   -4.1,\n",
       "   -7.5,\n",
       "   -5.2,\n",
       "   -7.2,\n",
       "   -6.6,\n",
       "   -5.6,\n",
       "   -8.0,\n",
       "   -9.4,\n",
       "   -7.2,\n",
       "   -5.2,\n",
       "   -11.2,\n",
       "   -11.0,\n",
       "   -12.3,\n",
       "   -16.6,\n",
       "   0.9,\n",
       "   -1.3],\n",
       "  'UniProt_ID': 'P00720',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 34.06,\n",
       "  'pH': 3.05,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '19384988',\n",
       "  'KEY_WORDS': 'Bacteriophage T4 lysozyme; electrostatics; strain; temperature-sensitive mutant',\n",
       "  'REFERENCE': 'PROTEIN SCI 18, 871-880 (2009) PMID: 19384988'},\n",
       " 'Q13526-7.0': {'MUTATION': ['K6A',\n",
       "   'L7A',\n",
       "   'L7I',\n",
       "   'L7V',\n",
       "   'L7N',\n",
       "   'P8A',\n",
       "   'P8G',\n",
       "   'P9A',\n",
       "   'P9G',\n",
       "   'G10A',\n",
       "   'W11F',\n",
       "   'E12A',\n",
       "   'K13A',\n",
       "   'R14A',\n",
       "   'M15A',\n",
       "   'S16A',\n",
       "   'S16G',\n",
       "   'R17A',\n",
       "   'R17G',\n",
       "   'S18A',\n",
       "   'S18G',\n",
       "   'S19A',\n",
       "   'S19G',\n",
       "   'G20A',\n",
       "   'R21A',\n",
       "   'R21G',\n",
       "   'V22A',\n",
       "   'Y23A',\n",
       "   'Y23L',\n",
       "   'Y23F',\n",
       "   'Y24F',\n",
       "   'Y24W',\n",
       "   'F25A',\n",
       "   'F25L',\n",
       "   'F25Y',\n",
       "   'N26D',\n",
       "   'H27A',\n",
       "   'H27G',\n",
       "   'I28A',\n",
       "   'I28G',\n",
       "   'T29A',\n",
       "   'T29G',\n",
       "   'T29S',\n",
       "   'T29D',\n",
       "   'N30A',\n",
       "   'N30G',\n",
       "   'A31G',\n",
       "   'S32A',\n",
       "   'S32G',\n",
       "   'Q33A',\n",
       "   'W34A',\n",
       "   'W34F',\n",
       "   'E35A',\n",
       "   'R36A',\n",
       "   'S38A',\n",
       "   'S38G'],\n",
       "  '∆Tm': [0.8,\n",
       "   -20.8,\n",
       "   -9.3,\n",
       "   -14.6,\n",
       "   -9.8,\n",
       "   -11.2,\n",
       "   -10.9,\n",
       "   -2.6,\n",
       "   -5.5,\n",
       "   -9.6,\n",
       "   -23.6,\n",
       "   -6.0,\n",
       "   1.0,\n",
       "   -19.4,\n",
       "   -6.8,\n",
       "   -4.6,\n",
       "   -11.0,\n",
       "   0.2,\n",
       "   -1.3,\n",
       "   -0.2,\n",
       "   -2.1,\n",
       "   -1.5,\n",
       "   -2.6,\n",
       "   -9.7,\n",
       "   -7.7,\n",
       "   -7.1,\n",
       "   -4.4,\n",
       "   -24.7,\n",
       "   -13.3,\n",
       "   -5.8,\n",
       "   -7.2,\n",
       "   -5.7,\n",
       "   -26.1,\n",
       "   -16.1,\n",
       "   3.4,\n",
       "   -22.6,\n",
       "   -0.9,\n",
       "   -8.1,\n",
       "   -4.4,\n",
       "   -11.4,\n",
       "   -14.3,\n",
       "   -24.2,\n",
       "   -7.8,\n",
       "   -15.7,\n",
       "   -5.3,\n",
       "   6.4,\n",
       "   -17.7,\n",
       "   -1.7,\n",
       "   -8.5,\n",
       "   -5.5,\n",
       "   -5.7,\n",
       "   -0.6,\n",
       "   -8.3,\n",
       "   -1.9,\n",
       "   0.2,\n",
       "   -0.4],\n",
       "  'UniProt_ID': 'Q13526',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 79.73,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '19565466',\n",
       "  'KEY_WORDS': 'WW domain; Ala scanning; Gly scanning; side chain mutagenesis; protein stability; protein folding; beta-sheet',\n",
       "  'REFERENCE': 'PROTEIN SCI 18, 1806-1813 (2009) PMID: 19565466'},\n",
       " 'P03050-7.5': {'MUTATION': ['E48A',\n",
       "   'N29A',\n",
       "   'S44A',\n",
       "   'P8A',\n",
       "   'S35A',\n",
       "   'N11A',\n",
       "   'K6A',\n",
       "   'R16A',\n",
       "   'V25A',\n",
       "   'M4A',\n",
       "   'E27A',\n",
       "   'K2A',\n",
       "   'Q9A',\n",
       "   'G3A',\n",
       "   'M1A',\n",
       "   'S5A',\n",
       "   'R13A',\n",
       "   'E17A',\n",
       "   'V18A',\n",
       "   'E43A',\n",
       "   'M7A',\n",
       "   'D20A',\n",
       "   'L19A',\n",
       "   'F10A',\n",
       "   'N34A',\n",
       "   'Q39A',\n",
       "   'G52A',\n",
       "   'K46A',\n",
       "   'R23A',\n",
       "   'K24A',\n",
       "   'E28A',\n",
       "   'I51A',\n",
       "   'G49A',\n",
       "   'G30A',\n",
       "   'R50A',\n",
       "   'K47A',\n",
       "   'P15A',\n",
       "   'V33A',\n",
       "   'L12A',\n",
       "   'L21A',\n",
       "   'R31A',\n",
       "   'M42A',\n",
       "   'S32A',\n",
       "   'Y38A',\n",
       "   'W14A',\n",
       "   'R40A',\n",
       "   'K24T',\n",
       "   'L19Q',\n",
       "   'P8L'],\n",
       "  '∆Tm': [1.6,\n",
       "   2.5,\n",
       "   7.5,\n",
       "   15.1,\n",
       "   4.4,\n",
       "   3.1,\n",
       "   0.6,\n",
       "   0.5,\n",
       "   0.3,\n",
       "   0.2,\n",
       "   -0.2,\n",
       "   -0.3,\n",
       "   -0.6,\n",
       "   -0.9,\n",
       "   -1.0,\n",
       "   -1.5,\n",
       "   -1.7,\n",
       "   -2.0,\n",
       "   -2.1,\n",
       "   -2.9,\n",
       "   -3.5,\n",
       "   -3.7,\n",
       "   -11.7,\n",
       "   -19.6,\n",
       "   5.1,\n",
       "   3.5,\n",
       "   3.0,\n",
       "   -0.8,\n",
       "   -1.2,\n",
       "   -1.6,\n",
       "   -2.2,\n",
       "   -7.0,\n",
       "   -9.2,\n",
       "   -10.0,\n",
       "   -10.0,\n",
       "   -10.7,\n",
       "   -11.3,\n",
       "   -13.8,\n",
       "   -15.6,\n",
       "   -18.3,\n",
       "   -20.8,\n",
       "   -22.3,\n",
       "   -24.4,\n",
       "   -24.9,\n",
       "   -26.4,\n",
       "   -26.7,\n",
       "   -10.0,\n",
       "   -19.0,\n",
       "   13.9],\n",
       "  'UniProt_ID': 'P03050',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 84.14,\n",
       "  'pH': 7.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '8672461',\n",
       "  'KEY_WORDS': 'Arc repressor; hydrophobic interactions; salt-bridge; electrostatic;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 35, 7621-7625 (1996) PMID: 8672461'},\n",
       " 'P00974-7.0': {'MUTATION': ['R36A',\n",
       "   'P37A',\n",
       "   'D38A',\n",
       "   'F39A',\n",
       "   'L41A',\n",
       "   'E42A',\n",
       "   'P43A',\n",
       "   'P44A',\n",
       "   'Y45A',\n",
       "   'T46A',\n",
       "   'G47A',\n",
       "   'P48A',\n",
       "   'K50A',\n",
       "   'R52A',\n",
       "   'I53A',\n",
       "   'I54A',\n",
       "   'R55A',\n",
       "   'F57A',\n",
       "   'N59A',\n",
       "   'K61A',\n",
       "   'G63A',\n",
       "   'L64A',\n",
       "   'Q66A',\n",
       "   'T67A',\n",
       "   'V69A',\n",
       "   'Y70A',\n",
       "   'G71A',\n",
       "   'G72A',\n",
       "   'R74A',\n",
       "   'K76A',\n",
       "   'R77A',\n",
       "   'N79A',\n",
       "   'K81A',\n",
       "   'S82A',\n",
       "   'E84A',\n",
       "   'D85A',\n",
       "   'M87A',\n",
       "   'R88A',\n",
       "   'T89A',\n",
       "   'G91A',\n",
       "   'G92A'],\n",
       "  '∆Tm': [-3.9,\n",
       "   -9.8,\n",
       "   1.4,\n",
       "   -21.0,\n",
       "   -4.6,\n",
       "   -11.2,\n",
       "   -2.5,\n",
       "   -5.5,\n",
       "   -9.0,\n",
       "   -0.2,\n",
       "   -13.1,\n",
       "   -9.0,\n",
       "   -2.6,\n",
       "   -1.9,\n",
       "   -10.9,\n",
       "   -15.1,\n",
       "   -13.2,\n",
       "   -14.7,\n",
       "   -15.8,\n",
       "   -0.1,\n",
       "   -7.3,\n",
       "   0.3,\n",
       "   -7.5,\n",
       "   -0.4,\n",
       "   -9.0,\n",
       "   -8.3,\n",
       "   -15.4,\n",
       "   -17.2,\n",
       "   0.0,\n",
       "   -3.0,\n",
       "   -3.6,\n",
       "   -29.0,\n",
       "   0.6,\n",
       "   -11.9,\n",
       "   -1.3,\n",
       "   -2.6,\n",
       "   -12.1,\n",
       "   -0.9,\n",
       "   -0.7,\n",
       "   -1.4,\n",
       "   -1.5],\n",
       "  'UniProt_ID': 'P00974',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 67.69,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '7540212',\n",
       "  'KEY_WORDS': 'BPTI; protein folding; protein stability',\n",
       "  'REFERENCE': 'J MOL BIOL 249, 388-397 (1995) PMID: 7540212'},\n",
       " 'P06654-5.2': {'MUTATION': ['T279Y',\n",
       "   'T279I',\n",
       "   'T279F',\n",
       "   'T279W',\n",
       "   'T279V',\n",
       "   'T279S',\n",
       "   'T279M',\n",
       "   'T279C',\n",
       "   'T279L',\n",
       "   'T279R',\n",
       "   'T279N',\n",
       "   'T279H',\n",
       "   'T279Q',\n",
       "   'T279K',\n",
       "   'T279E',\n",
       "   'T279D',\n",
       "   'T279G'],\n",
       "  '∆Tm': [12.17,\n",
       "   10.73,\n",
       "   10.63,\n",
       "   8.68,\n",
       "   8.42,\n",
       "   7.75,\n",
       "   7.21,\n",
       "   6.94,\n",
       "   5.42,\n",
       "   5.36,\n",
       "   4.83,\n",
       "   3.91,\n",
       "   3.85,\n",
       "   3.6,\n",
       "   1.76,\n",
       "   -6.14,\n",
       "   -11.1],\n",
       "  'UniProt_ID': 'P06654',\n",
       "  'SEC_STR': 'Turn',\n",
       "  'ASA': 26.68,\n",
       "  'pH': 5.2,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8180173',\n",
       "  'KEY_WORDS': 'thermodynamic scale; beta-sheet; amino acids; protein G;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 33, 5510-5517 (1994) PMID: 8180173'},\n",
       " 'P00044-4.6': {'MUTATION': ['P82L',\n",
       "   'P82G',\n",
       "   'P82V',\n",
       "   'P82R',\n",
       "   'P82S',\n",
       "   'P82Y',\n",
       "   'P82W',\n",
       "   'L100I',\n",
       "   'Y103F',\n",
       "   'F16Y',\n",
       "   'L100V',\n",
       "   'F16I',\n",
       "   'F16W',\n",
       "   'L100T',\n",
       "   'F16M',\n",
       "   'L100A',\n",
       "   'Y103A',\n",
       "   'N58I',\n",
       "   'N58Q',\n",
       "   'N58S',\n",
       "   'N58H',\n",
       "   'N58T',\n",
       "   'N58A',\n",
       "   'N58V',\n",
       "   'N58M',\n",
       "   'N58L'],\n",
       "  '∆Tm': [-4.9,\n",
       "   -5.0,\n",
       "   -5.2,\n",
       "   -4.9,\n",
       "   -5.7,\n",
       "   -6.8,\n",
       "   -6.5,\n",
       "   1.3,\n",
       "   1.0,\n",
       "   -1.9,\n",
       "   -4.0,\n",
       "   -4.3,\n",
       "   -5.2,\n",
       "   -13.9,\n",
       "   -15.2,\n",
       "   -16.2,\n",
       "   -21.7,\n",
       "   13.0,\n",
       "   2.0,\n",
       "   1.8,\n",
       "   -1.7,\n",
       "   5.3,\n",
       "   4.5,\n",
       "   8.8,\n",
       "   13.4,\n",
       "   12.9],\n",
       "  'UniProt_ID': 'P00044',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 67.65,\n",
       "  'pH': 4.6,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '9485396',\n",
       "  'KEY_WORDS': 'iso-1-cytochrome c; proline; glycine; melting temperature;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 37, 2477-2487 (1998); doi:10.1021/bi972279a PMID: 9485396'},\n",
       " 'P00750-8.0': {'MUTATION': ['W288S',\n",
       "   'W288L',\n",
       "   'W288F',\n",
       "   'W288Y',\n",
       "   'Y290A',\n",
       "   'Y290E',\n",
       "   'Y290F',\n",
       "   'Y290K',\n",
       "   'Y290L',\n",
       "   'Y290Q',\n",
       "   'Y290W'],\n",
       "  '∆Tm': [-3.5,\n",
       "   -3.7,\n",
       "   -1.7,\n",
       "   -2.4,\n",
       "   -11.1,\n",
       "   -16.1,\n",
       "   -8.9,\n",
       "   -13.9,\n",
       "   -10.3,\n",
       "   -11.3,\n",
       "   -10.8],\n",
       "  'UniProt_ID': 'P00750',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 25.78,\n",
       "  'pH': 8.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': '-',\n",
       "  'PubMed_ID': '1554717',\n",
       "  'KEY_WORDS': 'Tissue plasminogen activator; kringle;Lysine',\n",
       "  'REFERENCE': 'Biochemistry 31(13), 3326–3335 (1992) PMID: 1554717'},\n",
       " 'P01053-6.3': {'MUTATION': ['S32A',\n",
       "   'E34N',\n",
       "   'K37A',\n",
       "   'K38A',\n",
       "   'K38G',\n",
       "   'K44A',\n",
       "   'L28A',\n",
       "   'V39A',\n",
       "   'I40V',\n",
       "   'I49V',\n",
       "   'I50V',\n",
       "   'V71A',\n",
       "   'I77V',\n",
       "   'T23A',\n",
       "   'V54A',\n",
       "   'V54T',\n",
       "   'L52A',\n",
       "   'L52V',\n",
       "   'F70L'],\n",
       "  '∆Tm': [-8.4,\n",
       "   -3.8,\n",
       "   -12.6,\n",
       "   -5.2,\n",
       "   -10.8,\n",
       "   -5.8,\n",
       "   -22.5,\n",
       "   -4.7,\n",
       "   -14.8,\n",
       "   -7.2,\n",
       "   1.4,\n",
       "   -16.8,\n",
       "   1.1,\n",
       "   -2.9,\n",
       "   -2.8,\n",
       "   -8.3,\n",
       "   -9.1,\n",
       "   -0.5,\n",
       "   -14.1],\n",
       "  'UniProt_ID': 'P01053',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 15.36,\n",
       "  'pH': 6.3,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '7849029',\n",
       "  'KEY_WORDS': 'protein engineering; hydrophobic clusters; secondary structure;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 34, 1695-1701 (1995) PMID: 7849029'},\n",
       " 'P00974-3.0': {'MUTATION': ['I53A',\n",
       "   'F68A',\n",
       "   'G47A',\n",
       "   'Y70A',\n",
       "   'G72A',\n",
       "   'G71A',\n",
       "   'K50A',\n",
       "   'R52A',\n",
       "   'I54A',\n",
       "   'T46A',\n",
       "   'P48A',\n",
       "   'R55A',\n",
       "   'V69A',\n",
       "   'R74A',\n",
       "   'K81A'],\n",
       "  '∆Tm': [0.2,\n",
       "   -16.5,\n",
       "   -16.1,\n",
       "   -16.2,\n",
       "   -4.2,\n",
       "   -11.7,\n",
       "   -4.2,\n",
       "   -4.7,\n",
       "   -4.8,\n",
       "   -0.3,\n",
       "   0.0,\n",
       "   0.0,\n",
       "   0.0,\n",
       "   0.0,\n",
       "   0.0],\n",
       "  'UniProt_ID': 'P00974',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 32.43,\n",
       "  'pH': 3.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8784199',\n",
       "  'KEY_WORDS': 'alpha-chymotrypsin; alanine; binding affinity;',\n",
       "  'REFERENCE': 'BIOCHEMISTRY 35, 11435-11446 (1996) PMID: 8784199'},\n",
       " 'R9S082-9.6': {'MUTATION': ['G130A',\n",
       "   'V67A',\n",
       "   'P89A',\n",
       "   'H117A',\n",
       "   'V14A',\n",
       "   'T68A',\n",
       "   'S118A',\n",
       "   'L12A',\n",
       "   'L10A',\n",
       "   'G24A',\n",
       "   'L138A',\n",
       "   'V115A',\n",
       "   'T52A',\n",
       "   'L150A',\n",
       "   'I143A'],\n",
       "  '∆Tm': [2.0,\n",
       "   1.4,\n",
       "   1.1,\n",
       "   0.3,\n",
       "   -1.3,\n",
       "   -0.5,\n",
       "   -0.5,\n",
       "   -0.8,\n",
       "   -0.8,\n",
       "   -2.2,\n",
       "   -3.6,\n",
       "   -2.9,\n",
       "   -2.8,\n",
       "   -3.2,\n",
       "   -3.9],\n",
       "  'UniProt_ID': 'R9S082',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 35.58,\n",
       "  'pH': 9.6,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '8358293',\n",
       "  'KEY_WORDS': 'alpha-helix; helix capping; protein folding; secondary',\n",
       "  'REFERENCE': 'PROTEIN SCI 2, 1099-1105 (1993) PMID: 8358293'},\n",
       " 'P00711-7.4': {'MUTATION': ['H51Y',\n",
       "   'H51A',\n",
       "   'L129H',\n",
       "   'L129R',\n",
       "   'L129E',\n",
       "   'Q136A',\n",
       "   'W137Y',\n",
       "   'W137H',\n",
       "   'A125S',\n",
       "   'H126Y',\n",
       "   'H126A',\n",
       "   'H126W',\n",
       "   'K133N',\n",
       "   'K133Q',\n",
       "   'K133E',\n",
       "   'V61N',\n",
       "   'V61A',\n",
       "   'V61G',\n",
       "   'Q73A',\n",
       "   'I78W',\n",
       "   'Y122P',\n",
       "   'Y122A',\n",
       "   'W123Y'],\n",
       "  '∆Tm': [0.3,\n",
       "   -11.6,\n",
       "   6.3,\n",
       "   1.9,\n",
       "   -1.1,\n",
       "   -4.1,\n",
       "   -5.4,\n",
       "   -3.3,\n",
       "   -6.1,\n",
       "   -1.0,\n",
       "   -4.1,\n",
       "   -8.7,\n",
       "   10.7,\n",
       "   -2.7,\n",
       "   -3.2,\n",
       "   -1.1,\n",
       "   -4.5,\n",
       "   -5.2,\n",
       "   -1.9,\n",
       "   -4.5,\n",
       "   -1.1,\n",
       "   -10.7,\n",
       "   -12.5],\n",
       "  'UniProt_ID': 'P00711',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 42.33,\n",
       "  'pH': 7.4,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes(>95%)',\n",
       "  'PubMed_ID': '10436084',\n",
       "  'KEY_WORDS': 'flexibility; alpha-lactalbumin; lactose synthase;',\n",
       "  'REFERENCE': 'PROTEIN ENG 12, 581-587 (1999) PMID: 10436084'},\n",
       " 'P69488-9.0': {'MUTATION': ['S11V',\n",
       "   'S11A',\n",
       "   'S11I',\n",
       "   'G45Q',\n",
       "   'E59L',\n",
       "   'E59K',\n",
       "   'E59Q',\n",
       "   'Y60T',\n",
       "   'E61V',\n",
       "   'E61T',\n",
       "   'E61H',\n",
       "   'Q73V',\n",
       "   'Y86D',\n",
       "   'T17K',\n",
       "   'S48D',\n",
       "   'C39D',\n",
       "   'S48K',\n",
       "   'H72K',\n",
       "   'S82K',\n",
       "   'S82R',\n",
       "   'T88R',\n",
       "   'Q87K',\n",
       "   'S110R'],\n",
       "  '∆Tm': [15.1,\n",
       "   16.5,\n",
       "   12.8,\n",
       "   -7.8,\n",
       "   10.3,\n",
       "   14.3,\n",
       "   8.4,\n",
       "   2.0,\n",
       "   13.5,\n",
       "   8.7,\n",
       "   4.4,\n",
       "   4.1,\n",
       "   -13.1,\n",
       "   21.6,\n",
       "   19.8,\n",
       "   19.8,\n",
       "   26.6,\n",
       "   32.8,\n",
       "   31.3,\n",
       "   31.5,\n",
       "   32.0,\n",
       "   31.2,\n",
       "   31.7],\n",
       "  'UniProt_ID': 'P69488',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 6.83,\n",
       "  'pH': 9.0,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'no',\n",
       "  'PubMed_ID': '20639520',\n",
       "  'KEY_WORDS': 'cutA1; thermal; guanidine; DSC',\n",
       "  'REFERENCE': 'J Biochem 148(4), 449-58 (2010) PMID: 20639520'},\n",
       " 'P01857-7.4': {'MUTATION': ['L125C',\n",
       "   'V146A',\n",
       "   'Q230E',\n",
       "   'S237P',\n",
       "   'K243E',\n",
       "   'K243Q',\n",
       "   'K275R',\n",
       "   'S283F',\n",
       "   'Q301L',\n",
       "   'N304D',\n",
       "   'S307T',\n",
       "   'T320I',\n",
       "   'Q321K',\n",
       "   'Q321R'],\n",
       "  '∆Tm': [20.0,\n",
       "   -2.6,\n",
       "   -0.8,\n",
       "   -0.6,\n",
       "   -0.8,\n",
       "   -0.3,\n",
       "   -1.0,\n",
       "   -0.4,\n",
       "   -0.7,\n",
       "   -0.3,\n",
       "   -0.5,\n",
       "   -1.3,\n",
       "   -1.7,\n",
       "   -1.6],\n",
       "  'UniProt_ID': 'P01857',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 7.65,\n",
       "  'pH': 7.4,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': '-',\n",
       "  'PubMed_ID': '21669873',\n",
       "  'KEY_WORDS': 'Ch2 domain; m01s; engineered;',\n",
       "  'REFERENCE': 'J Biol Chem 286(31), 27288-93 (2011) PMID:21669873'},\n",
       " 'P04925-7.0': {'MUTATION': ['L108F',\n",
       "   'T189V',\n",
       "   'T191A',\n",
       "   'T191V',\n",
       "   'T191P',\n",
       "   'H186R',\n",
       "   'E195K',\n",
       "   'H186Y',\n",
       "   'Y154H',\n",
       "   'F197H'],\n",
       "  '∆Tm': [-9.2, -9.2, -8.5, -9.4, -4.5, -4.3, -1.5, 3.8, 2.8, -10.7],\n",
       "  'UniProt_ID': 'P04925',\n",
       "  'SEC_STR': '-',\n",
       "  'ASA': nan,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '27982059',\n",
       "  'KEY_WORDS': 'mouse; prp; prion; recombinant; amyloid',\n",
       "  'REFERENCE': 'Sci Rep 6, 38877 () (2016) PMID: 27982059'},\n",
       " 'P08168-7.0': {'MUTATION': ['R175A',\n",
       "   'G297A',\n",
       "   'V247A',\n",
       "   'F380A',\n",
       "   'D303A',\n",
       "   'A307G',\n",
       "   'F13A',\n",
       "   'R66A',\n",
       "   'D296A',\n",
       "   'G144A',\n",
       "   'R171A',\n",
       "   'V170A',\n",
       "   'T304A',\n",
       "   'L111A',\n",
       "   'Y25A',\n",
       "   'V11A',\n",
       "   'R382A',\n",
       "   'V376A',\n",
       "   'L173A',\n",
       "   'E341A',\n",
       "   'R291A',\n",
       "   'F375A',\n",
       "   'V44A',\n",
       "   'K298A',\n",
       "   'F377A'],\n",
       "  '∆Tm': [-11.7,\n",
       "   -11.6,\n",
       "   -9.9,\n",
       "   -9.0,\n",
       "   -9.0,\n",
       "   -6.9,\n",
       "   -6.8,\n",
       "   -5.7,\n",
       "   -4.8,\n",
       "   -4.4,\n",
       "   -4.0,\n",
       "   -4.0,\n",
       "   -3.9,\n",
       "   -3.2,\n",
       "   -2.9,\n",
       "   -2.6,\n",
       "   -2.4,\n",
       "   -1.8,\n",
       "   -1.8,\n",
       "   -1.6,\n",
       "   -1.1,\n",
       "   -0.9,\n",
       "   -0.1,\n",
       "   0.1,\n",
       "   1.8],\n",
       "  'UniProt_ID': 'P08168',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 8.3,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '30679635',\n",
       "  'KEY_WORDS': 'Arrestin-1; fusion protein;recombinant; mcherry fusion',\n",
       "  'REFERENCE': 'Sci Rep 9(1), 439 (2019) PMID: 30679635'},\n",
       " 'P0ABQ4-7.8': {'MUTATION': ['D27F',\n",
       "   'T113V',\n",
       "   'Q108D',\n",
       "   'S138Y',\n",
       "   'D116F',\n",
       "   'T68N',\n",
       "   'E120P',\n",
       "   'V119F',\n",
       "   'S135I',\n",
       "   'C152I',\n",
       "   'H114R',\n",
       "   'S49E',\n",
       "   'H141E',\n",
       "   'E157F',\n",
       "   'G15W',\n",
       "   'L156Y',\n",
       "   'E139V',\n",
       "   'D87P',\n",
       "   'G43P',\n",
       "   'W74F',\n",
       "   'G67H',\n",
       "   'A6I',\n",
       "   'H141F',\n",
       "   'I115A',\n",
       "   'I115V',\n",
       "   'I155A',\n",
       "   'I155L',\n",
       "   'I155T',\n",
       "   'I61V',\n",
       "   'I91L',\n",
       "   'I91V',\n",
       "   'L112V',\n",
       "   'V40A',\n",
       "   'V75H',\n",
       "   'V75I',\n",
       "   'V88I',\n",
       "   'W133F'],\n",
       "  '∆Tm': [7.6,\n",
       "   3.9,\n",
       "   1.6,\n",
       "   1.5,\n",
       "   1.4,\n",
       "   1.4,\n",
       "   1.2,\n",
       "   0.8,\n",
       "   0.7,\n",
       "   0.1,\n",
       "   0.0,\n",
       "   -0.6,\n",
       "   -1.7,\n",
       "   -1.8,\n",
       "   -2.8,\n",
       "   -2.8,\n",
       "   -3.1,\n",
       "   -3.1,\n",
       "   -3.1,\n",
       "   -3.6,\n",
       "   -6.0,\n",
       "   -6.9,\n",
       "   -1.1,\n",
       "   -5.6,\n",
       "   -0.3,\n",
       "   -13.2,\n",
       "   -5.9,\n",
       "   -8.3,\n",
       "   1.7,\n",
       "   -10.3,\n",
       "   -2.6,\n",
       "   -4.3,\n",
       "   -8.5,\n",
       "   -11.1,\n",
       "   -12.5,\n",
       "   -7.4,\n",
       "   -5.4],\n",
       "  'UniProt_ID': 'P0ABQ4',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 15.96,\n",
       "  'pH': 7.8,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'no',\n",
       "  'PubMed_ID': '25905910',\n",
       "  'KEY_WORDS': 'DHFR; Ecoli; non-equlibrium',\n",
       "  'REFERENCE': 'PLoS Comput. Biol. 11(4), e1004207 (2015) PMID: 25905910'},\n",
       " 'D4Z2G1-7.5': {'MUTATION': ['L3S',\n",
       "   'A5E',\n",
       "   'A5L',\n",
       "   'K6E',\n",
       "   'E15T',\n",
       "   'R20L',\n",
       "   'E26H',\n",
       "   'E26I',\n",
       "   'E26Q',\n",
       "   'E26R',\n",
       "   'E26V',\n",
       "   'D30A',\n",
       "   'D30E',\n",
       "   'D30H',\n",
       "   'D30V',\n",
       "   'Q35I',\n",
       "   'Q35L',\n",
       "   'Q35V',\n",
       "   'S41Q',\n",
       "   'S42A',\n",
       "   'A53K',\n",
       "   'A53L',\n",
       "   'C61P',\n",
       "   'D68H',\n",
       "   'D68L',\n",
       "   'D68N',\n",
       "   'E78H',\n",
       "   'E78T',\n",
       "   'A81E',\n",
       "   'A81K',\n",
       "   'A81N',\n",
       "   'A81P',\n",
       "   'A83L',\n",
       "   'A83N',\n",
       "   'R86L',\n",
       "   'D87K',\n",
       "   'D87L',\n",
       "   'A95H',\n",
       "   'D97K',\n",
       "   'D97N',\n",
       "   'D97R',\n",
       "   'D100T',\n",
       "   'R101H',\n",
       "   'V103I',\n",
       "   'V105I',\n",
       "   'V106L',\n",
       "   'G114A',\n",
       "   'E123S',\n",
       "   'A129V',\n",
       "   'A133V',\n",
       "   'E139H',\n",
       "   'E139K',\n",
       "   'E139Q',\n",
       "   'E139T',\n",
       "   'D147H',\n",
       "   'D147L',\n",
       "   'D147M',\n",
       "   'D147Y',\n",
       "   'D149K',\n",
       "   'D149Q',\n",
       "   'Q152L',\n",
       "   'A153K',\n",
       "   'A153L',\n",
       "   'D166K',\n",
       "   'D166N',\n",
       "   'G176A',\n",
       "   'G176N',\n",
       "   'S183D',\n",
       "   'A185P',\n",
       "   'A189F',\n",
       "   'A189Y',\n",
       "   'E192K',\n",
       "   'E192L',\n",
       "   'E192R',\n",
       "   'A197P',\n",
       "   'A200P',\n",
       "   'A214G',\n",
       "   'A218E',\n",
       "   'A218P',\n",
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       "   'R225L',\n",
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       "   'G229A',\n",
       "   'G229D',\n",
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       "   'G229R',\n",
       "   'G229S',\n",
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       "   'E244V',\n",
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       "   'T264Q',\n",
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       "   'F169V',\n",
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       "   'Q210N',\n",
       "   'V220M',\n",
       "   'A247F',\n",
       "   'L248F',\n",
       "   'L248W',\n",
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       "   'T249M',\n",
       "   'T249V',\n",
       "   'A271I',\n",
       "   'A271V'],\n",
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       "   15.0,\n",
       "   14.0,\n",
       "   16.5,\n",
       "   11.0,\n",
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       "   10.0,\n",
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       "   2.5,\n",
       "   2.0,\n",
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       "   -0.5,\n",
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       "   0.5,\n",
       "   0.5,\n",
       "   0.5,\n",
       "   0.5,\n",
       "   -1.0,\n",
       "   -0.5,\n",
       "   -0.5,\n",
       "   -1.5,\n",
       "   0.5,\n",
       "   -0.5,\n",
       "   -0.5,\n",
       "   -2.5,\n",
       "   -1.0,\n",
       "   -0.5,\n",
       "   -1.0,\n",
       "   -1.0,\n",
       "   -3.0,\n",
       "   -3.0,\n",
       "   -1.0,\n",
       "   -0.5,\n",
       "   13.0,\n",
       "   10.5,\n",
       "   7.5,\n",
       "   10.0,\n",
       "   16.5,\n",
       "   10.5,\n",
       "   11.0,\n",
       "   11.5,\n",
       "   9.5,\n",
       "   9.0,\n",
       "   9.5,\n",
       "   11.0],\n",
       "  'UniProt_ID': 'D4Z2G1',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 35.5,\n",
       "  'pH': 7.5,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'no',\n",
       "  'PubMed_ID': '24976371',\n",
       "  'KEY_WORDS': 'computational design · co-solvents ·  dehalogenases; directed evolution; thermostability; virtual  screening',\n",
       "  'REFERENCE': 'ChemBioChem 15(11), 1660–1672 (2014) PMID: 24976371'},\n",
       " 'Q9ZEU2-7.0': {'MUTATION': ['D152A',\n",
       "   'D152E',\n",
       "   'D152I',\n",
       "   'Y155A',\n",
       "   'Y155F',\n",
       "   'Y155N',\n",
       "   'H195A',\n",
       "   'H195C',\n",
       "   'H195D',\n",
       "   'H195F',\n",
       "   'H195G',\n",
       "   'H195I',\n",
       "   'H195K',\n",
       "   'H195L',\n",
       "   'H195M',\n",
       "   'H195N',\n",
       "   'H195Q',\n",
       "   'H195R',\n",
       "   'H195S',\n",
       "   'H195T',\n",
       "   'H195V',\n",
       "   'H195W',\n",
       "   'H195Y',\n",
       "   'F258A',\n",
       "   'F258N',\n",
       "   'F258Y',\n",
       "   'R292D',\n",
       "   'R292H',\n",
       "   'R292K',\n",
       "   'R292V',\n",
       "   'H400A',\n",
       "   'H400C',\n",
       "   'H400E',\n",
       "   'H400F',\n",
       "   'H400G',\n",
       "   'H400I',\n",
       "   'H400K',\n",
       "   'H400L',\n",
       "   'H400M',\n",
       "   'H400N',\n",
       "   'H400P',\n",
       "   'H400Q',\n",
       "   'H400S',\n",
       "   'H400T',\n",
       "   'H400V',\n",
       "   'H400W',\n",
       "   'H400Y',\n",
       "   'R454A',\n",
       "   'R454E',\n",
       "   'R454F',\n",
       "   'D515A',\n",
       "   'D515I',\n",
       "   'R517A'],\n",
       "  '∆Tm': [0.2,\n",
       "   -1.4,\n",
       "   -1.6,\n",
       "   -1.0,\n",
       "   -0.2,\n",
       "   -1.0,\n",
       "   -1.6,\n",
       "   -0.2,\n",
       "   -3.4,\n",
       "   -3.6,\n",
       "   -2.8,\n",
       "   -6.6,\n",
       "   -0.4,\n",
       "   -6.4,\n",
       "   -0.2,\n",
       "   -1.4,\n",
       "   -3.0,\n",
       "   -5.0,\n",
       "   -0.8,\n",
       "   -4.0,\n",
       "   -4.0,\n",
       "   -5.8,\n",
       "   -3.2,\n",
       "   -3.4,\n",
       "   -3.2,\n",
       "   -0.8,\n",
       "   1.6,\n",
       "   0.4,\n",
       "   1.6,\n",
       "   1.8,\n",
       "   1.0,\n",
       "   0.8,\n",
       "   0.4,\n",
       "   -2.4,\n",
       "   0.8,\n",
       "   -0.2,\n",
       "   -1.6,\n",
       "   -0.4,\n",
       "   0.4,\n",
       "   -0.2,\n",
       "   2.2,\n",
       "   -0.4,\n",
       "   0.2,\n",
       "   0.4,\n",
       "   0.2,\n",
       "   0.4,\n",
       "   2.0,\n",
       "   -0.2,\n",
       "   -2.2,\n",
       "   0.2,\n",
       "   0.8,\n",
       "   0.6,\n",
       "   0.0],\n",
       "  'UniProt_ID': 'Q9ZEU2',\n",
       "  'SEC_STR': 'Turn',\n",
       "  'ASA': 11.1,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '24115119',\n",
       "  'KEY_WORDS': 'amylosucrase; GH 13 family; enzyme engineering',\n",
       "  'REFERENCE': 'Protein Sci. 22(12), 1754–1765 (2013) PMID: 24115119'},\n",
       " 'O25949-7.2': {'MUTATION': ['E153A',\n",
       "   'S154A',\n",
       "   'E155A',\n",
       "   'K157A',\n",
       "   'W159F',\n",
       "   'Q160A',\n",
       "   'K161A',\n",
       "   'L162A',\n",
       "   'C163A',\n",
       "   'S164A',\n",
       "   'E156A',\n",
       "   'W159A',\n",
       "   'D126A'],\n",
       "  '∆Tm': [-0.2,\n",
       "   -1.0,\n",
       "   -8.3,\n",
       "   -2.3,\n",
       "   -5.2,\n",
       "   -5.6,\n",
       "   -2.5,\n",
       "   -2.7,\n",
       "   -0.5,\n",
       "   0.7,\n",
       "   -9.0,\n",
       "   -17.0,\n",
       "   -7.0],\n",
       "  'UniProt_ID': 'O25949',\n",
       "  'SEC_STR': 'Sheet',\n",
       "  'ASA': 100.17,\n",
       "  'pH': 7.2,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '23270419',\n",
       "  'KEY_WORDS': 'Arginase; Helicobacter pyroli; non-conserved motif',\n",
       "  'REFERENCE': 'Biochemistry 52(3),508-19 (2013) PMID: 23270419'},\n",
       " 'P23540-7.5': {'MUTATION': ['Y102A',\n",
       "   'F103A',\n",
       "   'A106L',\n",
       "   'D108A',\n",
       "   'P126A',\n",
       "   'G128A',\n",
       "   'G144A',\n",
       "   'V167A',\n",
       "   'G174A',\n",
       "   'F191A'],\n",
       "  '∆Tm': [-17.2, -13.5, -7.51, -0.8, -2.14, -2.67, -3.88, -5.97, -0.2, -8.28],\n",
       "  'UniProt_ID': 'P23540',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 7.5,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '15322360',\n",
       "  'KEY_WORDS': 'Ribonuclease; T2; C-terminal; MC1; Rnase',\n",
       "  'REFERENCE': 'Biosci Biotechnol Biochem 68(8), 1748-57 (2004) PMID: 15322360'},\n",
       " 'O61594-6.0': {'MUTATION': ['L100A',\n",
       "   'L350A',\n",
       "   'L389A',\n",
       "   'Y390A',\n",
       "   'N391A',\n",
       "   'S247A',\n",
       "   'N249A',\n",
       "   'F251A',\n",
       "   'F334A',\n",
       "   'S358A',\n",
       "   'K366A',\n",
       "   'Y420A',\n",
       "   'D260A',\n",
       "   'E261A',\n",
       "   'M262F',\n",
       "   'A263F',\n",
       "   'E265A',\n",
       "   'L335A',\n",
       "   'V336F',\n",
       "   'S337F'],\n",
       "  '∆Tm': [0.4,\n",
       "   -2.5,\n",
       "   0.4,\n",
       "   -3.0,\n",
       "   -1.6,\n",
       "   -0.3,\n",
       "   -7.5,\n",
       "   -3.8,\n",
       "   -3.1,\n",
       "   -3.3,\n",
       "   -4.5,\n",
       "   -9.0,\n",
       "   -6.4,\n",
       "   -0.8,\n",
       "   -0.3,\n",
       "   -6.4,\n",
       "   -1.1,\n",
       "   -7.5,\n",
       "   -16.0,\n",
       "   -6.9],\n",
       "  'UniProt_ID': 'O61594',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 0.0,\n",
       "  'pH': 6.0,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': '-',\n",
       "  'PubMed_ID': '26785700',\n",
       "  'KEY_WORDS': 'residue interactio networks; beta glucosidase; denaturation; unfolding, transition temperature',\n",
       "  'REFERENCE': 'FEBS J. (2016) Mar;283(6):1124-38 PMID: 26785700'},\n",
       " 'P02549-7.4': {'MUTATION': ['I24S',\n",
       "   'I24T',\n",
       "   'R28C',\n",
       "   'R28H',\n",
       "   'R28L',\n",
       "   'R28S',\n",
       "   'V31A',\n",
       "   'R34W',\n",
       "   'R41W',\n",
       "   'R45S',\n",
       "   'R45T',\n",
       "   'G46V',\n",
       "   'K48R',\n",
       "   'L49F'],\n",
       "  '∆Tm': [-1.4,\n",
       "   -0.2,\n",
       "   1.0,\n",
       "   -1.0,\n",
       "   -1.5,\n",
       "   -1.3,\n",
       "   -0.1,\n",
       "   -5.5,\n",
       "   -1.2,\n",
       "   0.5,\n",
       "   0.0,\n",
       "   -1.8,\n",
       "   0.1,\n",
       "   0.8],\n",
       "  'UniProt_ID': 'P02549',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 40.54,\n",
       "  'pH': 7.4,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '18218854',\n",
       "  'KEY_WORDS': 'hereditary elliptocytosis (HE); hereditary pyropoikilocytosis; isothermal titration calorimetry; binding affinity; missense mutations',\n",
       "  'REFERENCE': 'Blood. (2008) Jun 15;111(12):5712-20 PMID: 18218854'},\n",
       " 'Q99972-6.0': {'MUTATION': ['G246R',\n",
       "   'G252R',\n",
       "   'R272G',\n",
       "   'D273A',\n",
       "   'E323K',\n",
       "   'G364V',\n",
       "   'G367R',\n",
       "   'T377M',\n",
       "   'D380A',\n",
       "   'D384A',\n",
       "   'E385A',\n",
       "   'K423E',\n",
       "   'V426F',\n",
       "   'A427T',\n",
       "   'C433R',\n",
       "   'Y437H',\n",
       "   'I477N',\n",
       "   'I477S',\n",
       "   'N480K',\n",
       "   'P481L',\n",
       "   'I499F',\n",
       "   'S502P'],\n",
       "  '∆Tm': [-10.5,\n",
       "   -10.0,\n",
       "   -12.0,\n",
       "   -31.3,\n",
       "   -9.0,\n",
       "   -8.0,\n",
       "   -10.3,\n",
       "   -8.7,\n",
       "   -6.4,\n",
       "   -10.5,\n",
       "   -13.3,\n",
       "   -18.8,\n",
       "   -11.5,\n",
       "   -4.7,\n",
       "   -12.6,\n",
       "   -12.7,\n",
       "   -15.3,\n",
       "   -13.3,\n",
       "   -10.6,\n",
       "   -7.5,\n",
       "   -10.2,\n",
       "   -12.0],\n",
       "  'UniProt_ID': 'Q99972',\n",
       "  'SEC_STR': 'Helix',\n",
       "  'ASA': 27.95,\n",
       "  'pH': 6.0,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'no',\n",
       "  'PubMed_ID': '23129764',\n",
       "  'KEY_WORDS': 'Calcium Binding Proteins; Extracellular Matrix Proteins; Eye; Metalloproteins; Protein Misfolding; Biomechanics; Glaucoma; Olfactomedin',\n",
       "  'REFERENCE': 'J Biol Chem. (2012) Dec 21; 287(52): 43370–43377 PMID: 23129764'},\n",
       " 'P03661-7.0': {'MUTATION': ['N33G',\n",
       "   'R47W',\n",
       "   'N57K',\n",
       "   'G73A',\n",
       "   'T74I',\n",
       "   'I78V',\n",
       "   'P83H',\n",
       "   'N156G',\n",
       "   'F217L',\n",
       "   'S225L',\n",
       "   'T31I',\n",
       "   'Q147H'],\n",
       "  '∆Tm': [-5.9,\n",
       "   2.2,\n",
       "   -5.2,\n",
       "   -7.5,\n",
       "   -10.4,\n",
       "   -9.6,\n",
       "   -11.2,\n",
       "   -5.9,\n",
       "   -8.3,\n",
       "   -9.0,\n",
       "   5.5,\n",
       "   4.2],\n",
       "  'UniProt_ID': 'P03661',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 42.35,\n",
       "  'pH': 7.0,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'yes',\n",
       "  'PubMed_ID': '18621056',\n",
       "  'KEY_WORDS': 'protein stabilization; disulfide bonds; evolutionary protein design; phage gene-3-protein; phage display',\n",
       "  'REFERENCE': 'J Mol Biol. (2008) Sep 12;381(4):1040-54 PMID: 18621056'},\n",
       " 'Q9ZAG3-7.1': {'MUTATION': ['S12R',\n",
       "   'K13P',\n",
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       "   'L106R',\n",
       "   'K110R',\n",
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       "   'E124R',\n",
       "   'I127F',\n",
       "   'A142R'],\n",
       "  '∆Tm': [0.2,\n",
       "   -4.0,\n",
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       "   -2.8],\n",
       "  'UniProt_ID': 'Q9ZAG3',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 32.42,\n",
       "  'pH': 7.1,\n",
       "  'STATE': '2',\n",
       "  'REVERSIBILITY': 'no',\n",
       "  'PubMed_ID': '24402331',\n",
       "  'KEY_WORDS': 'enzyme stability; in silico design; in silico screening; protein stability engineering; thermostability',\n",
       "  'REFERENCE': 'Protein Eng Des Sel. (2014) Feb; 27(2): 49–58 PMID: 24402331'},\n",
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       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '27999093',\n",
       "  'KEY_WORDS': 'biocatalysis; cosolvent stability; haloalcohol dehalogenase; protein engineering; thermostability',\n",
       "  'REFERENCE': 'Protein Eng Des Sel. (2017) Mar 1;30(3):173-187 PMID: 27999093'},\n",
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       "   'E245A',\n",
       "   'S246A',\n",
       "   'M247A',\n",
       "   'K248A',\n",
       "   'L249A',\n",
       "   'F250A',\n",
       "   'D251A',\n",
       "   'S252A',\n",
       "   'I253A',\n",
       "   'C254A',\n",
       "   'N255A',\n",
       "   'N256A',\n",
       "   'K257A',\n",
       "   'W258A',\n",
       "   'F259A',\n",
       "   'T260A',\n",
       "   'D261A',\n",
       "   'T262A',\n",
       "   'S263A',\n",
       "   'I264A',\n",
       "   'I265A',\n",
       "   'L266A',\n",
       "   'F267A',\n",
       "   'L268A',\n",
       "   'N269A',\n",
       "   'K271A',\n",
       "   'L273A',\n",
       "   'F274A',\n",
       "   'E275A',\n",
       "   'E276A',\n",
       "   'K277A',\n",
       "   'I278A',\n",
       "   'K279A',\n",
       "   'K280A',\n",
       "   'S281A',\n",
       "   'P282A',\n",
       "   'L283A',\n",
       "   'T284A',\n",
       "   'I285A',\n",
       "   'C286A',\n",
       "   'Y287A',\n",
       "   'P288A',\n",
       "   'E289A',\n",
       "   'Y290A',\n",
       "   'A291G',\n",
       "   'G292A',\n",
       "   'S293A',\n",
       "   'N294A',\n",
       "   'T295A',\n",
       "   'Y296A',\n",
       "   'E297A',\n",
       "   'E298A',\n",
       "   'A299G',\n",
       "   'A300G',\n",
       "   'A301G',\n",
       "   'Y302A',\n",
       "   'I303A',\n",
       "   'Q304A',\n",
       "   'C305A',\n",
       "   'Q306A',\n",
       "   'F307A',\n",
       "   'E308A',\n",
       "   'D309A',\n",
       "   'L310A',\n",
       "   'N311A',\n",
       "   'K312A',\n",
       "   'R313A',\n",
       "   'K314A',\n",
       "   'D315A',\n",
       "   'T316A',\n",
       "   'K317A',\n",
       "   'E318A',\n",
       "   'I319A',\n",
       "   'Y320A',\n",
       "   'T321A',\n",
       "   'H322A',\n",
       "   'F323A',\n",
       "   'T324A',\n",
       "   'C325A',\n",
       "   'A326G',\n",
       "   'T327A',\n",
       "   'D328A',\n",
       "   'T329A',\n",
       "   'K330A',\n",
       "   'N331A',\n",
       "   'V332A',\n",
       "   'Q333A',\n",
       "   'F334A',\n",
       "   'V335A',\n",
       "   'D337A',\n",
       "   'A338G',\n",
       "   'V339A',\n",
       "   'T340A',\n",
       "   'D341A',\n",
       "   'V342A',\n",
       "   'I343A',\n",
       "   'I344A',\n",
       "   'K345A',\n",
       "   'N346A',\n",
       "   'N347A',\n",
       "   'L348A',\n",
       "   'K349A',\n",
       "   'D350A',\n",
       "   'C351A',\n",
       "   'G352A',\n",
       "   'L353A',\n",
       "   'F354A'],\n",
       "  '∆Tm': [-0.2,\n",
       "   -0.3,\n",
       "   -0.2,\n",
       "   -0.2,\n",
       "   -1.0,\n",
       "   -0.2,\n",
       "   -0.2,\n",
       "   -0.3,\n",
       "   -0.4,\n",
       "   -0.3,\n",
       "   -0.2,\n",
       "   0.4,\n",
       "   -0.3,\n",
       "   -0.3,\n",
       "   -0.4,\n",
       "   -0.5,\n",
       "   -0.4,\n",
       "   -0.3,\n",
       "   -0.4,\n",
       "   -0.6,\n",
       "   -0.6,\n",
       "   -0.4,\n",
       "   -0.2,\n",
       "   -0.4,\n",
       "   0.0,\n",
       "   -0.1,\n",
       "   -0.5,\n",
       "   -0.1,\n",
       "   -0.8,\n",
       "   -0.4,\n",
       "   -0.4,\n",
       "   -0.9,\n",
       "   -1.5,\n",
       "   -2.6,\n",
       "   -1.3,\n",
       "   -4.1,\n",
       "   -1.2,\n",
       "   -10.7,\n",
       "   -0.2,\n",
       "   -16.8,\n",
       "   -1.9,\n",
       "   2.3,\n",
       "   -1.0,\n",
       "   0.0,\n",
       "   -0.6,\n",
       "   -8.7,\n",
       "   -1.1,\n",
       "   -9.8,\n",
       "   -18.2,\n",
       "   -1.2,\n",
       "   -8.3,\n",
       "   -3.4,\n",
       "   -8.2,\n",
       "   -7.4,\n",
       "   -8.0,\n",
       "   -9.7,\n",
       "   -0.5,\n",
       "   0.7,\n",
       "   3.6,\n",
       "   -2.3,\n",
       "   -7.6,\n",
       "   -1.9,\n",
       "   -1.0,\n",
       "   -0.8,\n",
       "   -3.2,\n",
       "   -3.2,\n",
       "   -0.9,\n",
       "   0.0,\n",
       "   -5.0,\n",
       "   -1.4,\n",
       "   -0.3,\n",
       "   0.3,\n",
       "   -6.4,\n",
       "   -3.6,\n",
       "   -0.1,\n",
       "   -2.6,\n",
       "   -1.5,\n",
       "   -2.6,\n",
       "   -0.9,\n",
       "   -0.7,\n",
       "   -2.6,\n",
       "   -1.1,\n",
       "   -2.0,\n",
       "   -6.4,\n",
       "   -4.2,\n",
       "   -1.3,\n",
       "   -2.2,\n",
       "   -5.6,\n",
       "   -0.4,\n",
       "   -1.9,\n",
       "   -4.2,\n",
       "   -1.1,\n",
       "   -5.1,\n",
       "   -0.4,\n",
       "   -8.9,\n",
       "   -0.6,\n",
       "   -1.3,\n",
       "   0.4,\n",
       "   -0.4,\n",
       "   -0.9,\n",
       "   -0.4,\n",
       "   0.2,\n",
       "   -6.0,\n",
       "   -2.4,\n",
       "   0.0,\n",
       "   0.0,\n",
       "   -4.3,\n",
       "   -1.2,\n",
       "   0.4,\n",
       "   -0.9,\n",
       "   -0.8,\n",
       "   0.0,\n",
       "   0.1,\n",
       "   0.2,\n",
       "   -0.4,\n",
       "   0.1,\n",
       "   -0.4,\n",
       "   -0.2,\n",
       "   -4.3,\n",
       "   -1.2,\n",
       "   -0.8,\n",
       "   -1.1,\n",
       "   -3.0,\n",
       "   -1.9,\n",
       "   -0.2,\n",
       "   -0.9,\n",
       "   -3.6,\n",
       "   -1.2,\n",
       "   -2.4,\n",
       "   -9.0,\n",
       "   -6.8,\n",
       "   -0.2,\n",
       "   -8.2,\n",
       "   -0.4,\n",
       "   -3.5,\n",
       "   -4.5,\n",
       "   -3.4,\n",
       "   -2.4,\n",
       "   -0.8,\n",
       "   -2.9,\n",
       "   -0.2,\n",
       "   -0.4,\n",
       "   -2.4,\n",
       "   -1.3,\n",
       "   0.4,\n",
       "   -0.4,\n",
       "   -0.9,\n",
       "   -0.4,\n",
       "   0.2,\n",
       "   -6.0,\n",
       "   -2.4,\n",
       "   0.0,\n",
       "   0.0,\n",
       "   -4.3,\n",
       "   -1.2,\n",
       "   0.4,\n",
       "   -0.9,\n",
       "   -0.8,\n",
       "   0.0,\n",
       "   0.1,\n",
       "   0.2,\n",
       "   -0.4,\n",
       "   0.1,\n",
       "   -0.4,\n",
       "   -0.2,\n",
       "   -4.3,\n",
       "   -1.2,\n",
       "   -6.3,\n",
       "   -4.8,\n",
       "   -2.1,\n",
       "   -0.7,\n",
       "   -13.4,\n",
       "   -5.7,\n",
       "   -13.9,\n",
       "   -5.6,\n",
       "   1.8,\n",
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       "   -1.4,\n",
       "   -1.1,\n",
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       "   -2.6,\n",
       "   -1.9,\n",
       "   -1.8,\n",
       "   -0.7,\n",
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       "   -11.4,\n",
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       "   -13.6,\n",
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       "   -0.3,\n",
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       "   -0.8,\n",
       "   -0.4,\n",
       "   -0.7,\n",
       "   -0.2,\n",
       "   -0.8,\n",
       "   -0.1,\n",
       "   -1.3,\n",
       "   -7.2,\n",
       "   -4.2,\n",
       "   -3.6,\n",
       "   0.6,\n",
       "   1.6,\n",
       "   -4.9,\n",
       "   -2.4,\n",
       "   -0.2,\n",
       "   -7.5,\n",
       "   -2.5,\n",
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       "   -0.3,\n",
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       "   -2.7,\n",
       "   -3.9,\n",
       "   -8.4,\n",
       "   -2.9,\n",
       "   -0.8,\n",
       "   -0.6,\n",
       "   -1.7,\n",
       "   -0.3,\n",
       "   -1.8,\n",
       "   -13.5,\n",
       "   -1.2,\n",
       "   -1.3,\n",
       "   -0.7,\n",
       "   -1.3,\n",
       "   -2.7,\n",
       "   -0.2,\n",
       "   -0.4,\n",
       "   0.3,\n",
       "   -1.7,\n",
       "   -0.5,\n",
       "   -1.4,\n",
       "   -0.3,\n",
       "   -1.5,\n",
       "   -3.7,\n",
       "   -0.4,\n",
       "   -3.8,\n",
       "   -6.2,\n",
       "   -6.6,\n",
       "   -21.2,\n",
       "   -4.5,\n",
       "   -5.7,\n",
       "   -8.5,\n",
       "   -0.2,\n",
       "   -0.5,\n",
       "   -4.9,\n",
       "   -8.1,\n",
       "   -0.9,\n",
       "   -1.9,\n",
       "   -2.1,\n",
       "   -2.3,\n",
       "   -13.3,\n",
       "   -1.9,\n",
       "   -2.7,\n",
       "   -1.8,\n",
       "   -11.6,\n",
       "   -2.7,\n",
       "   -0.5,\n",
       "   -9.0,\n",
       "   -0.1,\n",
       "   -2.9,\n",
       "   0.2,\n",
       "   -1.4,\n",
       "   -1.3,\n",
       "   -2.8,\n",
       "   1.6,\n",
       "   -1.3,\n",
       "   -0.5,\n",
       "   1.2,\n",
       "   -0.2,\n",
       "   -8.1,\n",
       "   -5.8,\n",
       "   -0.9,\n",
       "   0.2,\n",
       "   -0.9,\n",
       "   -8.8,\n",
       "   -1.5,\n",
       "   0.3,\n",
       "   -2.3,\n",
       "   -1.0,\n",
       "   -0.1,\n",
       "   0.6,\n",
       "   -0.6,\n",
       "   0.3,\n",
       "   0.1,\n",
       "   -1.7,\n",
       "   0.1,\n",
       "   -4.2,\n",
       "   -1.7,\n",
       "   -0.5,\n",
       "   -1.0,\n",
       "   -3.6,\n",
       "   -4.0,\n",
       "   -3.9,\n",
       "   -4.3,\n",
       "   -6.4,\n",
       "   -7.4,\n",
       "   -5.5,\n",
       "   0.2,\n",
       "   -2.8,\n",
       "   -8.2,\n",
       "   -0.9,\n",
       "   1.8,\n",
       "   0.4,\n",
       "   -0.8,\n",
       "   -2.8,\n",
       "   -3.0,\n",
       "   0.1,\n",
       "   -2.1,\n",
       "   -2.5,\n",
       "   -5.4,\n",
       "   -0.4,\n",
       "   -0.7,\n",
       "   -1.5,\n",
       "   -1.6,\n",
       "   -0.9,\n",
       "   -1.0,\n",
       "   -0.5,\n",
       "   -0.2,\n",
       "   -0.2,\n",
       "   0.0,\n",
       "   0.0],\n",
       "  'UniProt_ID': 'P63096',\n",
       "  'SEC_STR': 'Coil',\n",
       "  'ASA': 84.96,\n",
       "  'pH': 7.4,\n",
       "  'STATE': '-',\n",
       "  'REVERSIBILITY': 'Unknown',\n",
       "  'PubMed_ID': '26258638',\n",
       "  'KEY_WORDS': 'G-protein; GPCR; alanine mutations; rhodopsin-Gα i1 complex; inactive; active form; GDP-bound state',\n",
       "  'REFERENCE': 'Nat Struct Mol Biol 22(9):686-694 (2015) PMID: 26258638'}}"
      ]
     },
     "execution_count": 5,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "data = {}\n",
    "for index, row in df.iterrows():\n",
    "    name = f\"{row['UniProt_ID']}-{row['pH']}\"\n",
    "    if name in [\"--7.4\", \"--7.0\"]:\n",
    "        continue\n",
    "    mutant = row[\"MUTATION\"].split(\"(\")[0].strip()\n",
    "    try:\n",
    "        score = float(row[\"∆Tm_(C)\"].split(\"(\")[0].strip())\n",
    "    except:\n",
    "        continue\n",
    "    if not data.get(name):\n",
    "        data[name] = {\n",
    "            \"MUTATION\": [mutant], \"∆Tm\": [score],\n",
    "            \"UniProt_ID\": row[\"UniProt_ID\"],\n",
    "            \"SEC_STR\": row[\"SEC_STR\"], \"ASA\": row[\"ASA\"],\n",
    "            \"pH\": row[\"pH\"], \"STATE\": row[\"STATE\"],\n",
    "            \"REVERSIBILITY\": row[\"REVERSIBILITY\"], \"PubMed_ID\": row[\"PubMed_ID\"],\n",
    "            \"KEY_WORDS\": row[\"KEY_WORDS\"], \"REFERENCE\": row[\"REFERENCE\"],\n",
    "        }\n",
    "    else:\n",
    "        if mutant not in data[name][\"MUTATION\"]:\n",
    "            data[name][\"MUTATION\"].append(mutant)\n",
    "            data[name][\"∆Tm\"].append(score)\n",
    "data_ = {}\n",
    "for k, v in data.items():\n",
    "    if len(v[\"MUTATION\"]) >= 10:\n",
    "        data_[k] = v\n",
    "data_"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "id": "c6aa5ddd-6334-465d-9a18-5881f5a6e364",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "85"
      ]
     },
     "execution_count": 6,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "uids = []\n",
    "for k ,v in data_.items():\n",
    "    uids.append(k)\n",
    "len(uids)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 30,
   "id": "2d262bac-1147-4604-954c-d6f11d7a0aca",
   "metadata": {
    "scrolled": true
   },
   "outputs": [],
   "source": [
    "for k,v in data_.items():\n",
    "    os.makedirs(f\"data/{k}\", exist_ok=True)\n",
    "    os.makedirs(f\"data/{k}/experiments\", exist_ok=True)\n",
    "    shutil.copyfile(f\"/home/user4/workspace/ProteinCollector/data/tm/AF-{k.split('-')[0]}-F1-model_v4.pdb\", f\"data/{k}/{k}.pdb\")\n",
    "    coords, seq = load_coords(f\"data/{k}/{k}.pdb\", \"A\")\n",
    "    with open(f\"data/{k}/{k}.fasta\" ,\"w\") as f:\n",
    "        f.write(f\"> {name}\\n\")\n",
    "        f.write(seq)\n",
    "    with open(f\"data/{k}/{k}_detail.fasta\", \"w\") as f:\n",
    "        json.dump(v, f)\n",
    "    df = {\"mutant\": [], \"score\": []}\n",
    "    try:\n",
    "        for m, s in zip(v[\"MUTATION\"], v['∆Tm']):\n",
    "            src, pos, trg = m[0], int(m[1:-1])-1, m[-1]\n",
    "            assert seq[pos] == src, f\"{seq[pos]} != {src} at {pos}\"\n",
    "            df[\"mutant\"].append(m)\n",
    "            df[\"score\"].append(s)\n",
    "    except:\n",
    "        shutil.rmtree(f\"data/{k}\")\n",
    "        continue\n",
    "    df = pd.DataFrame(df)\n",
    "    df.to_csv(f\"data/{k}/experiments/{k}.tsv\", sep=\"\\t\", index=False)\n",
    "    \n",
    "    "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "id": "aa452b7e-09d6-4bf6-a9dd-f018e34dd0ff",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "(29, 14, 23)"
      ]
     },
     "execution_count": 2,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ddg = [f.strip() for f in open(\"result/tm_plg.txt\", \"r\").readlines()]\n",
    "ac, ne, al = 0, 0, 0\n",
    "for p in ddg:\n",
    "    if float(p.split('-')[1]) == 7:\n",
    "        ne +=1\n",
    "    elif float(p.split('-')[1]) < 7:\n",
    "        ac += 1\n",
    "    elif float(p.split('-')[1]) > 7:\n",
    "        al += 1\n",
    "ac, ne, al"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "id": "a929760a-00a0-4d0f-b1a7-971c7541420a",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "(29.06896551724138, 37.42857142857143, 50.130434782608695)"
      ]
     },
     "execution_count": 3,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ac_m, ne_m, al_m = [], [], []\n",
    "ac_l, ne_l, al_l, t_l = [], [], [], []\n",
    "for p in ddg:\n",
    "    df = pd.read_table(f\"data/{p}/experiments/{p}.tsv\")\n",
    "    lenth = len(open(f\"data/{p}/{p}.fasta\", \"r\").readlines()[1].strip())\n",
    "    if float(p.split('-')[1]) == 7:\n",
    "        ne_m.append(len(df))\n",
    "        ne_l.append(lenth)\n",
    "    elif float(p.split('-')[1]) < 7:\n",
    "        ac_m.append(len(df))\n",
    "        ac_l.append(lenth)\n",
    "    elif float(p.split('-')[1]) > 7:\n",
    "        al_m.append(len(df))\n",
    "        al_l.append(lenth)\n",
    "    t_l.append(lenth)\n",
    "np.mean(ac_m), np.mean(ne_m), np.mean(al_m)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "id": "0b75741d-b367-49fd-a3c0-07993141f39a",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "(272.82758620689657, 221.21428571428572, 233.30434782608697)"
      ]
     },
     "execution_count": 4,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "np.mean(ac_l), np.mean(ne_l), np.mean(al_l)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "id": "db19ad1b-3fef-4d7c-8814-be6e3ff6cad7",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "(685, 248.1060606060606, 53)"
      ]
     },
     "execution_count": 5,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "np.max(t_l), np.mean(t_l), np.min(t_l)"
   ]
  }
 ],
 "metadata": {
  "kernelspec": {
   "display_name": "protein",
   "language": "python",
   "name": "protein"
  },
  "language_info": {
   "codemirror_mode": {
    "name": "ipython",
    "version": 3
   },
   "file_extension": ".py",
   "mimetype": "text/x-python",
   "name": "python",
   "nbconvert_exporter": "python",
   "pygments_lexer": "ipython3",
   "version": "3.10.11"
  }
 },
 "nbformat": 4,
 "nbformat_minor": 5
}
